Endothelial cells use dynamic actin to facilitate lymphocyte transendothelial migration and maintain the monolayer barrier.
Bottom Line: The actin cytoskeleton of the endothelial cell (EC) is known to facilitate transmigration, but the cellular and molecular mechanisms are not well understood.We found that docking structure formation involves the localization and activation of Arp2/3 complex by WAVE2.Finally, we found that ECs in resting endothelial monolayers use lamellipodial protrusions dependent on WAVE2 to form and maintain contacts and junctions between cells.
Affiliation: Department of Cell Biology and Physiology, Washington University, St. Louis, MO 63110.Show MeSH
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Mentions: To begin TEM, lymphocytes adhere to the endothelial monolayer and walk along its apical surface (Supplemental Movie S1 and Figure 1A). Docking structures form on the EC and bind the lymphocyte, after which the lymphocyte passes through the endothelium via the paracellular or transcellular route. Supplemental Movie S1 and Figure 1A show an example of the latter.
Affiliation: Department of Cell Biology and Physiology, Washington University, St. Louis, MO 63110.