Olfactomedin 2, a novel regulator for transforming growth factor-β-induced smooth muscle differentiation of human embryonic stem cell-derived mesenchymal cells.
Bottom Line: Olfm2 also inhibited HERP1 expression.Moreover, blockade of Olfm2 expression inhibited TGF-β-induced SRF binding to SM gene promoters in a chromatin setting, whereas overexpression of Olfm2 dose dependently enhanced SRF binding.These results demonstrate that Olfm2 mediates TGF-β-induced SM gene transcription by empowering SRF binding to CArG box in SM gene promoters.
Affiliation: Department of Physiology and Pharmacology, University of Georgia, Athens, GA 30602.Show MeSH
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Mentions: Because Olfm2 function in SM differentiation relies on SRF/CArG, we sought to determine whether Olfm2 interacts with SRF. A coimmunoprecipitation (CoIP) assay using endogenous proteins extracted from hES-MCs treated with vehicle or TGF-β showed that Olfm2 was coimmunoprecipitated with SRF (Figure 5A) and SRF was coimmunoprecipitated with Olfm2 (Figure 5C), demonstrating that Olfm2 physically interacted with SRF in hES-MCs. Their interaction was significantly enhanced by TGF-β, which was likely attributable to the increased expression of Olfm2 by TGF-β (Figure 5, A–D).
Affiliation: Department of Physiology and Pharmacology, University of Georgia, Athens, GA 30602.