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Genome-wide Analysis of Mycoplasma hominis for the Identification of Putative Therapeutic Targets.

Parvege MM, Rahman M, Hossain MS - Drug Target Insights (2014)

Bottom Line: Our study also identified 179 essential proteins, including 59 proteins having no similarity with human proteins.Further filtering by molecular weight, subcellular localization, functional analysis, and protein network interaction, we identified 57 putative candidates for which new drugs can be developed.Druggability analysis for each of the identified targets has prioritized 16 proteins as suitable for potential drug development.

View Article: PubMed Central - PubMed

Affiliation: Department of Genetic Engineering & Biotechnology, University of Dhaka, Dhaka, Bangladesh.

ABSTRACT
Ever increasing propensity of antibiotic resistance among pathogenic bacteria raises the demand for the development of novel therapeutic agents to control this grave problem. Advances in the field of bioinformatics, genomics, and proteomics have greatly facilitated the discovery of alternative drugs by swift identification of new drug targets. In the present study, we employed comparative genomics and metabolic pathway analysis with an aim of identifying therapeutic targets in Mycoplasma hominis. Our study has revealed 40 annotated metabolic pathways, including five unique pathways of M. hominis. Our study also identified 179 essential proteins, including 59 proteins having no similarity with human proteins. Further filtering by molecular weight, subcellular localization, functional analysis, and protein network interaction, we identified 57 putative candidates for which new drugs can be developed. Druggability analysis for each of the identified targets has prioritized 16 proteins as suitable for potential drug development.

No MeSH data available.


Related in: MedlinePlus

Distribution of putative therapeutic targets in their associated pathways. The percentage distribution of other pathways ranges from 0 to 4.5.
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f3-dti-8-2014-051: Distribution of putative therapeutic targets in their associated pathways. The percentage distribution of other pathways ranges from 0 to 4.5.

Mentions: The increasing trend of bacterial resistance to antibiotics is posing an imminent public health concern. Researchers around the world are giving attention to find new drug targets so that bacterial infections can be prevented. A vast array of omics data and the availability of various computational tools have speeded up the therapeutic target identification process. The potential of a protein to be a therapeutic target depends on two factors, essentiality and absence in the host. Essential proteins are required for bacterial survival and blocking them inhibits bacterial growth. Non-homologous proteins are preferred because they reduce the probability of side effects. In this study, we extracted metabolic data from KEGG database and used different bioinformatics and computational databases and tools for the identification of appropriate therapeutic targets of M. hominis. Systematic computational analyses revealed 57 possible drug targets in M. hominis; among them 16 are druggable targets, which have homologs in DrugBank. We further utilized different drug prioritization parameters to make a short list of potential drug and vaccine targets. Predicted drug targets belong to a diverse range of cellular activity and fall mostly into ribosome synthesis, pyrimidine metabolism, homologous recombination, DNA replication, and ABC transporter pathways (Fig. 3).


Genome-wide Analysis of Mycoplasma hominis for the Identification of Putative Therapeutic Targets.

Parvege MM, Rahman M, Hossain MS - Drug Target Insights (2014)

Distribution of putative therapeutic targets in their associated pathways. The percentage distribution of other pathways ranges from 0 to 4.5.
© Copyright Policy - open-access
Related In: Results  -  Collection

Show All Figures
getmorefigures.php?uid=PMC4263438&req=5

f3-dti-8-2014-051: Distribution of putative therapeutic targets in their associated pathways. The percentage distribution of other pathways ranges from 0 to 4.5.
Mentions: The increasing trend of bacterial resistance to antibiotics is posing an imminent public health concern. Researchers around the world are giving attention to find new drug targets so that bacterial infections can be prevented. A vast array of omics data and the availability of various computational tools have speeded up the therapeutic target identification process. The potential of a protein to be a therapeutic target depends on two factors, essentiality and absence in the host. Essential proteins are required for bacterial survival and blocking them inhibits bacterial growth. Non-homologous proteins are preferred because they reduce the probability of side effects. In this study, we extracted metabolic data from KEGG database and used different bioinformatics and computational databases and tools for the identification of appropriate therapeutic targets of M. hominis. Systematic computational analyses revealed 57 possible drug targets in M. hominis; among them 16 are druggable targets, which have homologs in DrugBank. We further utilized different drug prioritization parameters to make a short list of potential drug and vaccine targets. Predicted drug targets belong to a diverse range of cellular activity and fall mostly into ribosome synthesis, pyrimidine metabolism, homologous recombination, DNA replication, and ABC transporter pathways (Fig. 3).

Bottom Line: Our study also identified 179 essential proteins, including 59 proteins having no similarity with human proteins.Further filtering by molecular weight, subcellular localization, functional analysis, and protein network interaction, we identified 57 putative candidates for which new drugs can be developed.Druggability analysis for each of the identified targets has prioritized 16 proteins as suitable for potential drug development.

View Article: PubMed Central - PubMed

Affiliation: Department of Genetic Engineering & Biotechnology, University of Dhaka, Dhaka, Bangladesh.

ABSTRACT
Ever increasing propensity of antibiotic resistance among pathogenic bacteria raises the demand for the development of novel therapeutic agents to control this grave problem. Advances in the field of bioinformatics, genomics, and proteomics have greatly facilitated the discovery of alternative drugs by swift identification of new drug targets. In the present study, we employed comparative genomics and metabolic pathway analysis with an aim of identifying therapeutic targets in Mycoplasma hominis. Our study has revealed 40 annotated metabolic pathways, including five unique pathways of M. hominis. Our study also identified 179 essential proteins, including 59 proteins having no similarity with human proteins. Further filtering by molecular weight, subcellular localization, functional analysis, and protein network interaction, we identified 57 putative candidates for which new drugs can be developed. Druggability analysis for each of the identified targets has prioritized 16 proteins as suitable for potential drug development.

No MeSH data available.


Related in: MedlinePlus