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rs833061 and rs699947 on promoter gene of vascular endothelial growth factor (VEGF) and associated lung cancer susceptibility and survival: a meta-analysis.

Tu J, Wang S, Zhao J, Zhu J, Sheng L, Sheng Y, Chen H, Tian J - Med. Sci. Monit. (2014)

Bottom Line: Similar results were observed in -2578C>A in Asians (AA vs.CC: OR=3.00, 95% CI 1.51-5.95, p=0.002; AA vs.AC: OR=3.15, 95% CI 1.00-9.91, p=0.05; AA vs. (AC+CC): OR=2.92, 95% CI 1.51-5.65, p=0.001).

View Article: PubMed Central - PubMed

Affiliation: Department of Pneumology, Zhejiang University Jinhua Hospital, Jinhua Municipal Central Hospital, Jinhua, Zhejiang, China (mainland).

ABSTRACT

Background: As 2 important SNPs located in the promoter region of VEGF gene, the roles of rs833061 (-460C>T) and rs699947 (-2578C>A) in lung cancer susceptibility and survival remain inconclusive and controversial.

Material/methods: For better understanding of these 2 SNPs in lung cancer risk and survival, a meta-analysis was performed to pool findings of previous studies and to generate large-scale evidence.

Results: Based on the 10 eligible studies included, this study observed that the -460C>T polymorphism generally had no significant effect on lung cancer risk. However, subgroup analysis found that -460TT homozygote variant might confer significantly increased cancer risk for Asians (TT vs. CC: OR=1.69, 95% CI 1.08-2.63, p=0.02), but not in Caucasians. Similar results were observed in -2578C>A in Asians (AA vs. CC: OR=3.00, 95% CI 1.51-5.95, p=0.002; AA vs. AC: OR=3.15, 95% CI 1.00-9.91, p=0.05; AA vs. (AC+CC): OR=2.92, 95% CI 1.51-5.65, p=0.001). In lung cancer survival, 4 trials included had conflicting results. One found -460C>T polymorphism had no effect on survival, 1 observed risk increasing, while the remaining 2 observed risk decreasing. This inconsistency was closely related to the different therapeutic practices applied in different studies, the effects of which were significantly affected by VEGF expression.

Conclusions: -460TT and -2578AA homozygote might lead to significantly increased cancer risk for Asians, but the effects on survival remain to be explored. These 2 SNPs might be potential indicators of lung cancer risk for Asians and should be considered when planning chemotherapy and radiotherapy for lung cancer patients.

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Related in: MedlinePlus

Flow diagram of the search process.
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f1-medscimonit-20-2520: Flow diagram of the search process.

Mentions: After searching and screening with preset criteria, a total of 10 studies were included for this meta-analysis. Four trials explored the association between rs833061 (−460C>T) and lung cancer risk [11,12,14,15]. Four trials explored the association between rs699947 (−2578C>A) and lung cancer risk [14,16–18]. Four studies studied the association between rs833061 (−460C>T) and lung cancer survival [13,14,19,20]. The key characteristics and quality scoring of the studies were given in Table 1. The search and screening process was summarized in Figure 1. A total of 6,614 patients and controls were involved in this study. Seven studies were conducted in Asians (5 in China, 1 in Korea, and 1 in Japan) and 3 were in Caucasians (2 in the US and 1 in Portugal). Six studies used polymerase chain reaction-restriction fragment length polymorphism (PCRRFLP) for genotyping, 3 studies used TaqMan method, and 1 used MassARRAY. The quality score of all studies was between 20 and 35, suggesting moderate-to-high quality. The genotype distribution of cases and controls is summarized in Table 2. All studies had p>0.05 in HWE test of control group, suggesting no population stratification.


rs833061 and rs699947 on promoter gene of vascular endothelial growth factor (VEGF) and associated lung cancer susceptibility and survival: a meta-analysis.

Tu J, Wang S, Zhao J, Zhu J, Sheng L, Sheng Y, Chen H, Tian J - Med. Sci. Monit. (2014)

Flow diagram of the search process.
© Copyright Policy
Related In: Results  -  Collection

Show All Figures
getmorefigures.php?uid=PMC4263404&req=5

f1-medscimonit-20-2520: Flow diagram of the search process.
Mentions: After searching and screening with preset criteria, a total of 10 studies were included for this meta-analysis. Four trials explored the association between rs833061 (−460C>T) and lung cancer risk [11,12,14,15]. Four trials explored the association between rs699947 (−2578C>A) and lung cancer risk [14,16–18]. Four studies studied the association between rs833061 (−460C>T) and lung cancer survival [13,14,19,20]. The key characteristics and quality scoring of the studies were given in Table 1. The search and screening process was summarized in Figure 1. A total of 6,614 patients and controls were involved in this study. Seven studies were conducted in Asians (5 in China, 1 in Korea, and 1 in Japan) and 3 were in Caucasians (2 in the US and 1 in Portugal). Six studies used polymerase chain reaction-restriction fragment length polymorphism (PCRRFLP) for genotyping, 3 studies used TaqMan method, and 1 used MassARRAY. The quality score of all studies was between 20 and 35, suggesting moderate-to-high quality. The genotype distribution of cases and controls is summarized in Table 2. All studies had p>0.05 in HWE test of control group, suggesting no population stratification.

Bottom Line: Similar results were observed in -2578C>A in Asians (AA vs.CC: OR=3.00, 95% CI 1.51-5.95, p=0.002; AA vs.AC: OR=3.15, 95% CI 1.00-9.91, p=0.05; AA vs. (AC+CC): OR=2.92, 95% CI 1.51-5.65, p=0.001).

View Article: PubMed Central - PubMed

Affiliation: Department of Pneumology, Zhejiang University Jinhua Hospital, Jinhua Municipal Central Hospital, Jinhua, Zhejiang, China (mainland).

ABSTRACT

Background: As 2 important SNPs located in the promoter region of VEGF gene, the roles of rs833061 (-460C>T) and rs699947 (-2578C>A) in lung cancer susceptibility and survival remain inconclusive and controversial.

Material/methods: For better understanding of these 2 SNPs in lung cancer risk and survival, a meta-analysis was performed to pool findings of previous studies and to generate large-scale evidence.

Results: Based on the 10 eligible studies included, this study observed that the -460C>T polymorphism generally had no significant effect on lung cancer risk. However, subgroup analysis found that -460TT homozygote variant might confer significantly increased cancer risk for Asians (TT vs. CC: OR=1.69, 95% CI 1.08-2.63, p=0.02), but not in Caucasians. Similar results were observed in -2578C>A in Asians (AA vs. CC: OR=3.00, 95% CI 1.51-5.95, p=0.002; AA vs. AC: OR=3.15, 95% CI 1.00-9.91, p=0.05; AA vs. (AC+CC): OR=2.92, 95% CI 1.51-5.65, p=0.001). In lung cancer survival, 4 trials included had conflicting results. One found -460C>T polymorphism had no effect on survival, 1 observed risk increasing, while the remaining 2 observed risk decreasing. This inconsistency was closely related to the different therapeutic practices applied in different studies, the effects of which were significantly affected by VEGF expression.

Conclusions: -460TT and -2578AA homozygote might lead to significantly increased cancer risk for Asians, but the effects on survival remain to be explored. These 2 SNPs might be potential indicators of lung cancer risk for Asians and should be considered when planning chemotherapy and radiotherapy for lung cancer patients.

Show MeSH
Related in: MedlinePlus