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Pericyte protection by edaravone after tissue plasminogen activator treatment in rat cerebral ischemia.

Deguchi K, Liu N, Liu W, Omote Y, Kono S, Yunoki T, Deguchi S, Yamashita T, Ikeda Y, Abe K - J. Neurosci. Res. (2014)

Bottom Line: Thus, tPA treatment damaged pericytes, resulting in the detachment from astrocytes and a decrease in glial cell line-derived neurotrophic factor secretion.However, treatment with edaravone greatly improved tPA-induced damage to pericytes.The present study demonstrates that exogenous tPA strongly damages pericytes and destroys the integrity of the NVU, but edaravone treatment can greatly ameliorate such damage after acute cerebral ischemia in rats.

View Article: PubMed Central - PubMed

Affiliation: Department of Neurology, Graduate School of Medicine, Dentistry and Pharmaceutical Sciences, Okayama University, Okayama, Japan.

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Related in: MedlinePlus

Double immunohistochemistry of PDGFRβ-positive pericytes (a–d, red), GDNF-positive cells (e–h, green), and merged images (i–l) at 4 days after tMCAO. Note that the fluorescent signals for GDNF were partially observed surrounding the PDGFRβ-positive pericytes in the V + V group (i), with weaker signals observed in the V + tPA group (j). Also note the preserved GDNF secretion and overlapping pericytes in E + tPA and E + V groups (k,l, arrowheads). Scale bar = 100 μm
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fig06: Double immunohistochemistry of PDGFRβ-positive pericytes (a–d, red), GDNF-positive cells (e–h, green), and merged images (i–l) at 4 days after tMCAO. Note that the fluorescent signals for GDNF were partially observed surrounding the PDGFRβ-positive pericytes in the V + V group (i), with weaker signals observed in the V + tPA group (j). Also note the preserved GDNF secretion and overlapping pericytes in E + tPA and E + V groups (k,l, arrowheads). Scale bar = 100 μm

Mentions: Figure 6 shows that fluorescent signals for GDNF (green) were partially observed surrounding the PDGFRβ-positive pericyte (red) in the peri-ischemic region of the V + V group (merged). In addition, GDNF signals obviously decreased in the V + tPA group, whereas strong overlapping yellow signals were observed in the peri-ischemic region of the E + tPA group and especially the E + V group (arrowheads).


Pericyte protection by edaravone after tissue plasminogen activator treatment in rat cerebral ischemia.

Deguchi K, Liu N, Liu W, Omote Y, Kono S, Yunoki T, Deguchi S, Yamashita T, Ikeda Y, Abe K - J. Neurosci. Res. (2014)

Double immunohistochemistry of PDGFRβ-positive pericytes (a–d, red), GDNF-positive cells (e–h, green), and merged images (i–l) at 4 days after tMCAO. Note that the fluorescent signals for GDNF were partially observed surrounding the PDGFRβ-positive pericytes in the V + V group (i), with weaker signals observed in the V + tPA group (j). Also note the preserved GDNF secretion and overlapping pericytes in E + tPA and E + V groups (k,l, arrowheads). Scale bar = 100 μm
© Copyright Policy - open-access
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC4263311&req=5

fig06: Double immunohistochemistry of PDGFRβ-positive pericytes (a–d, red), GDNF-positive cells (e–h, green), and merged images (i–l) at 4 days after tMCAO. Note that the fluorescent signals for GDNF were partially observed surrounding the PDGFRβ-positive pericytes in the V + V group (i), with weaker signals observed in the V + tPA group (j). Also note the preserved GDNF secretion and overlapping pericytes in E + tPA and E + V groups (k,l, arrowheads). Scale bar = 100 μm
Mentions: Figure 6 shows that fluorescent signals for GDNF (green) were partially observed surrounding the PDGFRβ-positive pericyte (red) in the peri-ischemic region of the V + V group (merged). In addition, GDNF signals obviously decreased in the V + tPA group, whereas strong overlapping yellow signals were observed in the peri-ischemic region of the E + tPA group and especially the E + V group (arrowheads).

Bottom Line: Thus, tPA treatment damaged pericytes, resulting in the detachment from astrocytes and a decrease in glial cell line-derived neurotrophic factor secretion.However, treatment with edaravone greatly improved tPA-induced damage to pericytes.The present study demonstrates that exogenous tPA strongly damages pericytes and destroys the integrity of the NVU, but edaravone treatment can greatly ameliorate such damage after acute cerebral ischemia in rats.

View Article: PubMed Central - PubMed

Affiliation: Department of Neurology, Graduate School of Medicine, Dentistry and Pharmaceutical Sciences, Okayama University, Okayama, Japan.

Show MeSH
Related in: MedlinePlus