Pericyte protection by edaravone after tissue plasminogen activator treatment in rat cerebral ischemia.
Bottom Line: Thus, tPA treatment damaged pericytes, resulting in the detachment from astrocytes and a decrease in glial cell line-derived neurotrophic factor secretion.However, treatment with edaravone greatly improved tPA-induced damage to pericytes.The present study demonstrates that exogenous tPA strongly damages pericytes and destroys the integrity of the NVU, but edaravone treatment can greatly ameliorate such damage after acute cerebral ischemia in rats.
Affiliation: Department of Neurology, Graduate School of Medicine, Dentistry and Pharmaceutical Sciences, Okayama University, Okayama, Japan.Show MeSH
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Mentions: The double-fluorescence study showed that PDGFRβ-positive cells (Fig. 4, red) were double positive (arrowheads), with a smaller number of Ki67-positive cells (Fig. 4, green) in the V + tPA group (68.1 ± 10.4/mm2, P < 0.01) than in the V + V group (128.5 ± 15.8/mm2). On the other hand, the double-positive cells increased greatly in the E + V group (204.2 ± 8.5/mm2, P < 0.01 vs. V + V and V + tPA groups) compared with the V + V and the V + tPA groups. In addition, the double-positive cells in the E + tPA group (139.9 ± 10.4/mm2) increased significantly (P < 0.01) more than the V + tPA group and decreased (P < 0.01) relative to the E + V group (Fig. 4B).
Affiliation: Department of Neurology, Graduate School of Medicine, Dentistry and Pharmaceutical Sciences, Okayama University, Okayama, Japan.