Limits...
Pericyte protection by edaravone after tissue plasminogen activator treatment in rat cerebral ischemia.

Deguchi K, Liu N, Liu W, Omote Y, Kono S, Yunoki T, Deguchi S, Yamashita T, Ikeda Y, Abe K - J. Neurosci. Res. (2014)

Bottom Line: The number of pericytes and the overlap with NAGO decreased with tPA but recovered with edaravone 4 days after tMCAO with proliferation.Thus, tPA treatment damaged pericytes, resulting in the detachment from astrocytes and a decrease in glial cell line-derived neurotrophic factor secretion.However, treatment with edaravone greatly improved tPA-induced damage to pericytes.

View Article: PubMed Central - PubMed

Affiliation: Department of Neurology, Graduate School of Medicine, Dentistry and Pharmaceutical Sciences, Okayama University, Okayama, Japan.

Show MeSH

Related in: MedlinePlus

A: Double immunohistochemistry of PDGFRβ-positive pericytes (a–d, red), Ki67-positive cells (e–h, green), and merged images (i–l, arrowheads in double-positive cells), with a decrease in the V + tPA group and an increase in the E + V and E + tPA groups (B). **P < 0.01 vs. V + V group, ##P < 0.01 vs. V + tPA group, and ††P < 0.01 vs. E + V group. Scale bar = 100 μm.
© Copyright Policy - open-access
Related In: Results  -  Collection

License
getmorefigures.php?uid=PMC4263311&req=5

fig04: A: Double immunohistochemistry of PDGFRβ-positive pericytes (a–d, red), Ki67-positive cells (e–h, green), and merged images (i–l, arrowheads in double-positive cells), with a decrease in the V + tPA group and an increase in the E + V and E + tPA groups (B). **P < 0.01 vs. V + V group, ##P < 0.01 vs. V + tPA group, and ††P < 0.01 vs. E + V group. Scale bar = 100 μm.

Mentions: The double-fluorescence study showed that PDGFRβ-positive cells (Fig. 4, red) were double positive (arrowheads), with a smaller number of Ki67-positive cells (Fig. 4, green) in the V + tPA group (68.1 ± 10.4/mm2, P < 0.01) than in the V + V group (128.5 ± 15.8/mm2). On the other hand, the double-positive cells increased greatly in the E + V group (204.2 ± 8.5/mm2, P < 0.01 vs. V + V and V + tPA groups) compared with the V + V and the V + tPA groups. In addition, the double-positive cells in the E + tPA group (139.9 ± 10.4/mm2) increased significantly (P < 0.01) more than the V + tPA group and decreased (P < 0.01) relative to the E + V group (Fig. 4B).


Pericyte protection by edaravone after tissue plasminogen activator treatment in rat cerebral ischemia.

Deguchi K, Liu N, Liu W, Omote Y, Kono S, Yunoki T, Deguchi S, Yamashita T, Ikeda Y, Abe K - J. Neurosci. Res. (2014)

A: Double immunohistochemistry of PDGFRβ-positive pericytes (a–d, red), Ki67-positive cells (e–h, green), and merged images (i–l, arrowheads in double-positive cells), with a decrease in the V + tPA group and an increase in the E + V and E + tPA groups (B). **P < 0.01 vs. V + V group, ##P < 0.01 vs. V + tPA group, and ††P < 0.01 vs. E + V group. Scale bar = 100 μm.
© Copyright Policy - open-access
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC4263311&req=5

fig04: A: Double immunohistochemistry of PDGFRβ-positive pericytes (a–d, red), Ki67-positive cells (e–h, green), and merged images (i–l, arrowheads in double-positive cells), with a decrease in the V + tPA group and an increase in the E + V and E + tPA groups (B). **P < 0.01 vs. V + V group, ##P < 0.01 vs. V + tPA group, and ††P < 0.01 vs. E + V group. Scale bar = 100 μm.
Mentions: The double-fluorescence study showed that PDGFRβ-positive cells (Fig. 4, red) were double positive (arrowheads), with a smaller number of Ki67-positive cells (Fig. 4, green) in the V + tPA group (68.1 ± 10.4/mm2, P < 0.01) than in the V + V group (128.5 ± 15.8/mm2). On the other hand, the double-positive cells increased greatly in the E + V group (204.2 ± 8.5/mm2, P < 0.01 vs. V + V and V + tPA groups) compared with the V + V and the V + tPA groups. In addition, the double-positive cells in the E + tPA group (139.9 ± 10.4/mm2) increased significantly (P < 0.01) more than the V + tPA group and decreased (P < 0.01) relative to the E + V group (Fig. 4B).

Bottom Line: The number of pericytes and the overlap with NAGO decreased with tPA but recovered with edaravone 4 days after tMCAO with proliferation.Thus, tPA treatment damaged pericytes, resulting in the detachment from astrocytes and a decrease in glial cell line-derived neurotrophic factor secretion.However, treatment with edaravone greatly improved tPA-induced damage to pericytes.

View Article: PubMed Central - PubMed

Affiliation: Department of Neurology, Graduate School of Medicine, Dentistry and Pharmaceutical Sciences, Okayama University, Okayama, Japan.

Show MeSH
Related in: MedlinePlus