In vitro passages impact on virulence of Saprolegnia parasitica to Atlantic salmon, Salmo salar L. parr.
Bottom Line: The effect of serial in vitro subculturing on three pathogenic strains of Saprolegnia parasitica was investigated.Two of the strains declined in virulence after 15 successive in vitro subcultures, whereas one did not.It reveals that some strains degenerate more rapidly than others when subjected to successive in vitro subculturing on glucose-yeast extract.
Affiliation: Norwegian Veterinary Institute, Oslo, Norway.Show MeSH
Related in: MedlinePlus
Mentions: Strain 5708 was also included despite the fact that we did not observe a loss of virulence after passage. While we found that all passages resulted in significantly higher mortality than the controls 14 days post-infection, again there were no significant differences (statistically) between 5708AP, 570815P and 5708AP15P (Fig. 6).