In vitro passages impact on virulence of Saprolegnia parasitica to Atlantic salmon, Salmo salar L. parr.
Bottom Line: The effect of serial in vitro subculturing on three pathogenic strains of Saprolegnia parasitica was investigated.Two of the strains declined in virulence after 15 successive in vitro subcultures, whereas one did not.It reveals that some strains degenerate more rapidly than others when subjected to successive in vitro subculturing on glucose-yeast extract.
Affiliation: Norwegian Veterinary Institute, Oslo, Norway.Show MeSH
Related in: MedlinePlus
Mentions: For strain 2736, all three passages, 2736AP, 273615P and 2736AP15P, were significantly different from the control at 14 days post-infection. 273615P also gave significantly lower CPI compared with the other two passages. There was no difference between 2736AP and 2736AP15P (Fig. 5).