In vitro passages impact on virulence of Saprolegnia parasitica to Atlantic salmon, Salmo salar L. parr.
Bottom Line: The effect of serial in vitro subculturing on three pathogenic strains of Saprolegnia parasitica was investigated.Two of the strains declined in virulence after 15 successive in vitro subcultures, whereas one did not.It reveals that some strains degenerate more rapidly than others when subjected to successive in vitro subculturing on glucose-yeast extract.
Affiliation: Norwegian Veterinary Institute, Oslo, Norway.Show MeSH
Related in: MedlinePlus
Mentions: Strain 5337AP produced visible, typical lesions in a few fish within 48 h post-challenge, and the first moribund fish were collected and killed at this time point (48 h post-challenge) (Fig. 1). By day 3, some infected fish were observed in 53375P and 533715P tanks (Fig. 1). The CPI for 5337AP was significantly higher than those in the 53375P and 533715P groups by day 11 (Fig. 1).