The genome landscape of the african green monkey kidney-derived vero cell line.
Bottom Line: In addition, an ∼59-Mb loss of heterozygosity around this deleted region suggested that the homozygosity of the deletion was established by a large-scale conversion.Moreover, a genomic analysis of Vero cells revealed a female Chlorocebus sabaeus origin and proviral variations of the endogenous simian type D retrovirus.These results revealed the genomic basis for the non-tumourigenic permanent Vero cell lineage susceptible to various pathogens and will be useful for generating new sub-lines and developing new tools in the quality control of Vero cells.
Affiliation: Division of Evolutionary Genetics, Department of Population Genetics, National Institute of Genetics, Mishima, Shizuoka 411-8540, Japan.Show MeSH
Mentions: The large deletions predicted by the massively parallel sequencing system were validated by genomic PCR. Regarding the 8.85-Mb deletion of chromosome 12, a set of PCR primers striding across the deletion junction produced a ∼230-bp amplicon from Vero cells, but not from normal AGM cells, while a PCR primer set designated for the AGM genome produced a predicted amplicon from normal AGM cells, but not from Vero cells (Fig. 3A). We tested two Vero cell lines, JCRB0111 and ATCC CCL81, and obtained identical results (Fig. 3A). In similar validation tests for another four large (>90 kb) predicted deletions (Supplementary Table S4), DNA fragments with breakpoint junctions were amplified from the Vero cell lines, but not from AGM PBMC for all these deletions, which confirmed the existence of these deletions in Vero cells (Fig. 3A; Supplementary Fig. S2). Four small (1–2 kb) predicted deletions were also confirmed to exist (Fig. 3B; Supplementary Fig. S2).Figure 3.
Affiliation: Division of Evolutionary Genetics, Department of Population Genetics, National Institute of Genetics, Mishima, Shizuoka 411-8540, Japan.