Fish oil supplementation induces expression of genes related to cell cycle, endoplasmic reticulum stress and apoptosis in peripheral blood mononuclear cells: a transcriptomic approach.
Bottom Line: Cell cycle, DNA packaging and chromosome organization are biological processes found to be upregulated after intake of fish oil compared to high oleic sunflower oil using a moderated t-test.The genes contributing to the significantly different gene sets in the subjects given fish oil compared with the control group are involved in cell cycle, endoplasmic reticulum (ER) stress and apoptosis.The increased expression of genes related to cell cycle, ER stress and apoptosis suggests that intake of fish oil may modulate basic cellular processes involved in normal cellular function.
Affiliation: Faculty of Health Sciences, Department of Health, Nutrition and Management, Oslo and Akershus University College of Applied Sciences, Oslo, Norway.Show MeSH
Mentions: Gene Set Enrichment Analysis was used to test whether groups of genes involved in the same biological process or pathway, or regulated by the same transcription factor, were changed after intake of FO compared with the control group among the 21 236 defined expressed genes. The relative changes from baseline after 7 weeks of intervention were compared in the two groups. Overall, 162 gene sets were significantly enriched (FDR q-value of <0.25; 72 with FDR q-value of <0.15) in the FO group compared with the HOSO group in the C2 cgp collection (MSigDB). These gene sets included several overlapping genes. Leading edge genes (1460 non-overlapping genes shown in Table S3) contributing to the significance of the regulated gene sets were further analysed for functional and biological pathways using MetaCore for better interpretation of the GSEA result. The results were ranked by their P-value and annotated onto the developed pathway maps. It is interesting that several pathways were significantly regulated among the leading edge genes. The top 10 pathway maps (FDR q-value of <0.05) were related to cell cycle, apoptosis, immune response, protein folding and maturation, and DNA damage (Table4). Metacore pathway maps of the Anaphase-promoting complex (APC) regulation of cell cycle and endoplasmic reticulum (ER) stress response are shown in Figs2 and 3, respectively.
Affiliation: Faculty of Health Sciences, Department of Health, Nutrition and Management, Oslo and Akershus University College of Applied Sciences, Oslo, Norway.