Fish oil supplementation induces expression of genes related to cell cycle, endoplasmic reticulum stress and apoptosis in peripheral blood mononuclear cells: a transcriptomic approach.
Bottom Line: Cell cycle, DNA packaging and chromosome organization are biological processes found to be upregulated after intake of fish oil compared to high oleic sunflower oil using a moderated t-test.The genes contributing to the significantly different gene sets in the subjects given fish oil compared with the control group are involved in cell cycle, endoplasmic reticulum (ER) stress and apoptosis.The increased expression of genes related to cell cycle, ER stress and apoptosis suggests that intake of fish oil may modulate basic cellular processes involved in normal cellular function.
Affiliation: Faculty of Health Sciences, Department of Health, Nutrition and Management, Oslo and Akershus University College of Applied Sciences, Oslo, Norway.Show MeSH
Mentions: This study was part of a randomized, controlled, double-blind, three-arm, parallel-group study, designed to investigate the health effects of FO intake 22, in which the transcriptome was analysed as a pre-defined endpoint. Subjects were randomly assigned and stratified by gender into three groups using equal randomization (1:1:1). Data from only two of the intervention groups were analysed in the present study (see Fig.1). Subjects were required to take 16 capsules per day containing a total of 8 g day−1 of either FO or high oleic sunflower oil (HOSO) for 7 weeks and were instructed to take the capsules with food (during a minimum of two meals). Subjects in the FO group received capsules containing a total of 0.7 g day−1 EPA + 0.9 g day−1 DHA from cod liver oil (Gadidae sp., EPA/DHA Oil 1200, TINE SA, Oslo, Norway) and subjects in the HOSO group received HOSO purchased from AarhusKarlshamn AB (Malmö, Sweden). The fatty acid composition of the oils has been described elsewhere 22. The capsule containers were identical, and the capsules were the same size and to a large extent similar in colour.
Affiliation: Faculty of Health Sciences, Department of Health, Nutrition and Management, Oslo and Akershus University College of Applied Sciences, Oslo, Norway.