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Tension force-induced ATP promotes osteogenesis through P2X7 receptor in osteoblasts.

Kariya T, Tanabe N, Shionome C, Manaka S, Kawato T, Zhao N, Maeno M, Suzuki N, Shimizu N - J. Cell. Biochem. (2015)

Bottom Line: TF significantly increased extracellular ATP release compared to control.Six percent TF had maximum effect on ATP release compared to 18% TF and control.A438079 blocked the stimulatory effect of 6% TF on the expression of Runx2, Osterix and ECMPs, ALP activity, and calcium content in ECM.

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Affiliation: Nihon University Graduate School of Dentistry, Tokyo, Japan.

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Effects of A438079 and/or 6% TF stimulation on ALP activity. Cells were stimulated with or without 6% TF stimulation for 3 h/day, and cultured in the presence or absence of A438079 (10 μM) for 14 days. Cells were stained by ALP activity staining. Cells were observed under a phase-contrast microscope (original magnification, ×100) (a). Histograms showed the percentage of ALPase activity under each condition (b). Data are shown as the mean ± SEM, n = 3 independent experiments. **P < 0.01, TF stimulation vs. control, +P < 0.05, ++P < 0.01, 6% TF stimulation vs. 6% TF stimulation + A438079.
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fig07: Effects of A438079 and/or 6% TF stimulation on ALP activity. Cells were stimulated with or without 6% TF stimulation for 3 h/day, and cultured in the presence or absence of A438079 (10 μM) for 14 days. Cells were stained by ALP activity staining. Cells were observed under a phase-contrast microscope (original magnification, ×100) (a). Histograms showed the percentage of ALPase activity under each condition (b). Data are shown as the mean ± SEM, n = 3 independent experiments. **P < 0.01, TF stimulation vs. control, +P < 0.05, ++P < 0.01, 6% TF stimulation vs. 6% TF stimulation + A438079.

Mentions: To investigate the role of 6% TF-induced ATP in mineralized nodule formation, we examined ALP activity and calcium content of ECM in the presence of A438079 and/or 6% TF stimulation on day 14 of culture. ALP activity was enhanced by 6% TF stimulation compared to control (Fig. 7). In contrast, A438079 reduced the stimulatory effect of 6% TF stimulation on ALP activity. 6% TF stimulation did not affect cell viability on 1 and 14 days of culture (Figs. 8a and b). Calcium content of ECM was increased by 6.7-fold in 6% TF stimulation compared to control on days 7 and 14 of culture (Fig. 8b). A438079 reduced the stimulatory effect of 6% TF stimulation on the calcium content.


Tension force-induced ATP promotes osteogenesis through P2X7 receptor in osteoblasts.

Kariya T, Tanabe N, Shionome C, Manaka S, Kawato T, Zhao N, Maeno M, Suzuki N, Shimizu N - J. Cell. Biochem. (2015)

Effects of A438079 and/or 6% TF stimulation on ALP activity. Cells were stimulated with or without 6% TF stimulation for 3 h/day, and cultured in the presence or absence of A438079 (10 μM) for 14 days. Cells were stained by ALP activity staining. Cells were observed under a phase-contrast microscope (original magnification, ×100) (a). Histograms showed the percentage of ALPase activity under each condition (b). Data are shown as the mean ± SEM, n = 3 independent experiments. **P < 0.01, TF stimulation vs. control, +P < 0.05, ++P < 0.01, 6% TF stimulation vs. 6% TF stimulation + A438079.
© Copyright Policy - open-access
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC4263259&req=5

fig07: Effects of A438079 and/or 6% TF stimulation on ALP activity. Cells were stimulated with or without 6% TF stimulation for 3 h/day, and cultured in the presence or absence of A438079 (10 μM) for 14 days. Cells were stained by ALP activity staining. Cells were observed under a phase-contrast microscope (original magnification, ×100) (a). Histograms showed the percentage of ALPase activity under each condition (b). Data are shown as the mean ± SEM, n = 3 independent experiments. **P < 0.01, TF stimulation vs. control, +P < 0.05, ++P < 0.01, 6% TF stimulation vs. 6% TF stimulation + A438079.
Mentions: To investigate the role of 6% TF-induced ATP in mineralized nodule formation, we examined ALP activity and calcium content of ECM in the presence of A438079 and/or 6% TF stimulation on day 14 of culture. ALP activity was enhanced by 6% TF stimulation compared to control (Fig. 7). In contrast, A438079 reduced the stimulatory effect of 6% TF stimulation on ALP activity. 6% TF stimulation did not affect cell viability on 1 and 14 days of culture (Figs. 8a and b). Calcium content of ECM was increased by 6.7-fold in 6% TF stimulation compared to control on days 7 and 14 of culture (Fig. 8b). A438079 reduced the stimulatory effect of 6% TF stimulation on the calcium content.

Bottom Line: TF significantly increased extracellular ATP release compared to control.Six percent TF had maximum effect on ATP release compared to 18% TF and control.A438079 blocked the stimulatory effect of 6% TF on the expression of Runx2, Osterix and ECMPs, ALP activity, and calcium content in ECM.

View Article: PubMed Central - PubMed

Affiliation: Nihon University Graduate School of Dentistry, Tokyo, Japan.

Show MeSH
Related in: MedlinePlus