Tension force-induced ATP promotes osteogenesis through P2X7 receptor in osteoblasts.
Bottom Line: TF significantly increased extracellular ATP release compared to control.Six percent TF had maximum effect on ATP release compared to 18% TF and control.A438079 blocked the stimulatory effect of 6% TF on the expression of Runx2, Osterix and ECMPs, ALP activity, and calcium content in ECM.
Affiliation: Nihon University Graduate School of Dentistry, Tokyo, Japan.Show MeSH
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Mentions: To investigate the role of 6% TF-induced ATP in mineralized nodule formation, we examined ALP activity and calcium content of ECM in the presence of A438079 and/or 6% TF stimulation on day 14 of culture. ALP activity was enhanced by 6% TF stimulation compared to control (Fig. 7). In contrast, A438079 reduced the stimulatory effect of 6% TF stimulation on ALP activity. 6% TF stimulation did not affect cell viability on 1 and 14 days of culture (Figs. 8a and b). Calcium content of ECM was increased by 6.7-fold in 6% TF stimulation compared to control on days 7 and 14 of culture (Fig. 8b). A438079 reduced the stimulatory effect of 6% TF stimulation on the calcium content.
Affiliation: Nihon University Graduate School of Dentistry, Tokyo, Japan.