Tension force-induced ATP promotes osteogenesis through P2X7 receptor in osteoblasts.
Bottom Line: TF significantly increased extracellular ATP release compared to control.Six percent TF had maximum effect on ATP release compared to 18% TF and control.A438079 blocked the stimulatory effect of 6% TF on the expression of Runx2, Osterix and ECMPs, ALP activity, and calcium content in ECM.
Affiliation: Nihon University Graduate School of Dentistry, Tokyo, Japan.Show MeSH
Related in: MedlinePlus
Mentions: In 6% TF stimulation, the mRNA expression of type I collagen, bone sialoprotein (BSP), osteopontin (OPN), and osteocalcin (OCN) was increased by 2.8-, 2.2-, 2.7-, and 2.2-fold, respectively, compared to control on days 3, 7, and/or 14 of culture (Fig. 5). Protein expressions were also increased by 6% TF stimulation as well as mRNA expressions (Fig. 6). A438079 blocked the stimulatory effect of 6% TF stimulation on each ECMP expression at mRNA and protein levels (Figs. 5 and 6).
Affiliation: Nihon University Graduate School of Dentistry, Tokyo, Japan.