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Tension force-induced ATP promotes osteogenesis through P2X7 receptor in osteoblasts.

Kariya T, Tanabe N, Shionome C, Manaka S, Kawato T, Zhao N, Maeno M, Suzuki N, Shimizu N - J. Cell. Biochem. (2015)

Bottom Line: TF significantly increased extracellular ATP release compared to control.Six percent TF had maximum effect on ATP release compared to 18% TF and control.A438079 blocked the stimulatory effect of 6% TF on the expression of Runx2, Osterix and ECMPs, ALP activity, and calcium content in ECM.

View Article: PubMed Central - PubMed

Affiliation: Nihon University Graduate School of Dentistry, Tokyo, Japan.

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Effects of A438079 and/or 6% TF stimulation on protein expression of ECMPs. Cells were stimulated with or without 6% TF stimulation for 3 h/day, and cultured in the presence or absence of A438079 (10 μM) for up to 14 days. The protein expression of type I collagen (a), BSP (b), OPN (c), and OCN (d) was determined by ELISA on days 3, 7, and 14 of culture. Data are shown as the mean ± SEM, n = 3 independent experiments. **P < 0.01, TF stimulation vs. control, +P < 0.05, ++P < 0.01, 6% TF stimulation vs. 6% TF stimulation + A438079.
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fig06: Effects of A438079 and/or 6% TF stimulation on protein expression of ECMPs. Cells were stimulated with or without 6% TF stimulation for 3 h/day, and cultured in the presence or absence of A438079 (10 μM) for up to 14 days. The protein expression of type I collagen (a), BSP (b), OPN (c), and OCN (d) was determined by ELISA on days 3, 7, and 14 of culture. Data are shown as the mean ± SEM, n = 3 independent experiments. **P < 0.01, TF stimulation vs. control, +P < 0.05, ++P < 0.01, 6% TF stimulation vs. 6% TF stimulation + A438079.

Mentions: In 6% TF stimulation, the mRNA expression of type I collagen, bone sialoprotein (BSP), osteopontin (OPN), and osteocalcin (OCN) was increased by 2.8-, 2.2-, 2.7-, and 2.2-fold, respectively, compared to control on days 3, 7, and/or 14 of culture (Fig. 5). Protein expressions were also increased by 6% TF stimulation as well as mRNA expressions (Fig. 6). A438079 blocked the stimulatory effect of 6% TF stimulation on each ECMP expression at mRNA and protein levels (Figs. 5 and 6).


Tension force-induced ATP promotes osteogenesis through P2X7 receptor in osteoblasts.

Kariya T, Tanabe N, Shionome C, Manaka S, Kawato T, Zhao N, Maeno M, Suzuki N, Shimizu N - J. Cell. Biochem. (2015)

Effects of A438079 and/or 6% TF stimulation on protein expression of ECMPs. Cells were stimulated with or without 6% TF stimulation for 3 h/day, and cultured in the presence or absence of A438079 (10 μM) for up to 14 days. The protein expression of type I collagen (a), BSP (b), OPN (c), and OCN (d) was determined by ELISA on days 3, 7, and 14 of culture. Data are shown as the mean ± SEM, n = 3 independent experiments. **P < 0.01, TF stimulation vs. control, +P < 0.05, ++P < 0.01, 6% TF stimulation vs. 6% TF stimulation + A438079.
© Copyright Policy - open-access
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC4263259&req=5

fig06: Effects of A438079 and/or 6% TF stimulation on protein expression of ECMPs. Cells were stimulated with or without 6% TF stimulation for 3 h/day, and cultured in the presence or absence of A438079 (10 μM) for up to 14 days. The protein expression of type I collagen (a), BSP (b), OPN (c), and OCN (d) was determined by ELISA on days 3, 7, and 14 of culture. Data are shown as the mean ± SEM, n = 3 independent experiments. **P < 0.01, TF stimulation vs. control, +P < 0.05, ++P < 0.01, 6% TF stimulation vs. 6% TF stimulation + A438079.
Mentions: In 6% TF stimulation, the mRNA expression of type I collagen, bone sialoprotein (BSP), osteopontin (OPN), and osteocalcin (OCN) was increased by 2.8-, 2.2-, 2.7-, and 2.2-fold, respectively, compared to control on days 3, 7, and/or 14 of culture (Fig. 5). Protein expressions were also increased by 6% TF stimulation as well as mRNA expressions (Fig. 6). A438079 blocked the stimulatory effect of 6% TF stimulation on each ECMP expression at mRNA and protein levels (Figs. 5 and 6).

Bottom Line: TF significantly increased extracellular ATP release compared to control.Six percent TF had maximum effect on ATP release compared to 18% TF and control.A438079 blocked the stimulatory effect of 6% TF on the expression of Runx2, Osterix and ECMPs, ALP activity, and calcium content in ECM.

View Article: PubMed Central - PubMed

Affiliation: Nihon University Graduate School of Dentistry, Tokyo, Japan.

Show MeSH
Related in: MedlinePlus