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Tension force-induced ATP promotes osteogenesis through P2X7 receptor in osteoblasts.

Kariya T, Tanabe N, Shionome C, Manaka S, Kawato T, Zhao N, Maeno M, Suzuki N, Shimizu N - J. Cell. Biochem. (2015)

Bottom Line: TF significantly increased extracellular ATP release compared to control.Six percent TF had maximum effect on ATP release compared to 18% TF and control.A438079 blocked the stimulatory effect of 6% TF on the expression of Runx2, Osterix and ECMPs, ALP activity, and calcium content in ECM.

View Article: PubMed Central - PubMed

Affiliation: Nihon University Graduate School of Dentistry, Tokyo, Japan.

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Effects of A438079 and/or 6% TF stimulation on gene expression of Runx2 and Osterix. Cells were cultured with or without 6% TF stimulation in the presence or absence of A438079 (10 μM). The mRNA expression of Runx2 (a) and Osterix (b) was determined using real-time PCR at hours 3 or 6 of culture, respectively. Data are shown as the mean ± SEM, n = 3 independent experiments.*P < 0.05, **P < 0.01, TF stimulation vs. control, ++P < 0.01, 6% TF stimulation vs. 6% TF stimulation + A438079.
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fig03: Effects of A438079 and/or 6% TF stimulation on gene expression of Runx2 and Osterix. Cells were cultured with or without 6% TF stimulation in the presence or absence of A438079 (10 μM). The mRNA expression of Runx2 (a) and Osterix (b) was determined using real-time PCR at hours 3 or 6 of culture, respectively. Data are shown as the mean ± SEM, n = 3 independent experiments.*P < 0.05, **P < 0.01, TF stimulation vs. control, ++P < 0.01, 6% TF stimulation vs. 6% TF stimulation + A438079.

Mentions: A438079 blocked the stimulatory effect of 6% TF stimulation on Runx2 and Osterix expressions at mRNA and protein levels (Figs. 3 and 4).


Tension force-induced ATP promotes osteogenesis through P2X7 receptor in osteoblasts.

Kariya T, Tanabe N, Shionome C, Manaka S, Kawato T, Zhao N, Maeno M, Suzuki N, Shimizu N - J. Cell. Biochem. (2015)

Effects of A438079 and/or 6% TF stimulation on gene expression of Runx2 and Osterix. Cells were cultured with or without 6% TF stimulation in the presence or absence of A438079 (10 μM). The mRNA expression of Runx2 (a) and Osterix (b) was determined using real-time PCR at hours 3 or 6 of culture, respectively. Data are shown as the mean ± SEM, n = 3 independent experiments.*P < 0.05, **P < 0.01, TF stimulation vs. control, ++P < 0.01, 6% TF stimulation vs. 6% TF stimulation + A438079.
© Copyright Policy - open-access
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC4263259&req=5

fig03: Effects of A438079 and/or 6% TF stimulation on gene expression of Runx2 and Osterix. Cells were cultured with or without 6% TF stimulation in the presence or absence of A438079 (10 μM). The mRNA expression of Runx2 (a) and Osterix (b) was determined using real-time PCR at hours 3 or 6 of culture, respectively. Data are shown as the mean ± SEM, n = 3 independent experiments.*P < 0.05, **P < 0.01, TF stimulation vs. control, ++P < 0.01, 6% TF stimulation vs. 6% TF stimulation + A438079.
Mentions: A438079 blocked the stimulatory effect of 6% TF stimulation on Runx2 and Osterix expressions at mRNA and protein levels (Figs. 3 and 4).

Bottom Line: TF significantly increased extracellular ATP release compared to control.Six percent TF had maximum effect on ATP release compared to 18% TF and control.A438079 blocked the stimulatory effect of 6% TF on the expression of Runx2, Osterix and ECMPs, ALP activity, and calcium content in ECM.

View Article: PubMed Central - PubMed

Affiliation: Nihon University Graduate School of Dentistry, Tokyo, Japan.

Show MeSH
Related in: MedlinePlus