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Tension force-induced ATP promotes osteogenesis through P2X7 receptor in osteoblasts.

Kariya T, Tanabe N, Shionome C, Manaka S, Kawato T, Zhao N, Maeno M, Suzuki N, Shimizu N - J. Cell. Biochem. (2015)

Bottom Line: TF significantly increased extracellular ATP release compared to control.Six percent TF had maximum effect on ATP release compared to 18% TF and control.A438079 blocked the stimulatory effect of 6% TF on the expression of Runx2, Osterix and ECMPs, ALP activity, and calcium content in ECM.

View Article: PubMed Central - PubMed

Affiliation: Nihon University Graduate School of Dentistry, Tokyo, Japan.

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Related in: MedlinePlus

Effects of TF stimulation on gene expression of Runx2, Osterix, and P2X7 receptor. Cells were stimulated with 0% (control), 6%, or 18% TF for up to 24 h. The mRNA expression of Runx2 (a), Osterix (b), and P2X7 (c) was determined using real-time PCR at hours 0.25, 0.5, 1, 3, 6, 12, and 24 of culture. Data are shown as the mean ± SEM, n = 3 independent experiments. *P < 0.05, **P < 0.01, TF stimulation vs. control.
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fig02: Effects of TF stimulation on gene expression of Runx2, Osterix, and P2X7 receptor. Cells were stimulated with 0% (control), 6%, or 18% TF for up to 24 h. The mRNA expression of Runx2 (a), Osterix (b), and P2X7 (c) was determined using real-time PCR at hours 0.25, 0.5, 1, 3, 6, 12, and 24 of culture. Data are shown as the mean ± SEM, n = 3 independent experiments. *P < 0.05, **P < 0.01, TF stimulation vs. control.

Mentions: In both 6% and 18% TF stimulation, the mRNA expression of Runx2 gradually increased through hour 3 of culture and decreased by hour 24 (Fig. 2a). The expression of Runx2 at 6% and 18% TF stimulation was significantly increased by 1.4–3.6 and 1.2–2.5-fold compared to control, respectively, at hours 0.25, 0.5, 1, 3, 6, 12, and/or 24 of culture.


Tension force-induced ATP promotes osteogenesis through P2X7 receptor in osteoblasts.

Kariya T, Tanabe N, Shionome C, Manaka S, Kawato T, Zhao N, Maeno M, Suzuki N, Shimizu N - J. Cell. Biochem. (2015)

Effects of TF stimulation on gene expression of Runx2, Osterix, and P2X7 receptor. Cells were stimulated with 0% (control), 6%, or 18% TF for up to 24 h. The mRNA expression of Runx2 (a), Osterix (b), and P2X7 (c) was determined using real-time PCR at hours 0.25, 0.5, 1, 3, 6, 12, and 24 of culture. Data are shown as the mean ± SEM, n = 3 independent experiments. *P < 0.05, **P < 0.01, TF stimulation vs. control.
© Copyright Policy - open-access
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC4263259&req=5

fig02: Effects of TF stimulation on gene expression of Runx2, Osterix, and P2X7 receptor. Cells were stimulated with 0% (control), 6%, or 18% TF for up to 24 h. The mRNA expression of Runx2 (a), Osterix (b), and P2X7 (c) was determined using real-time PCR at hours 0.25, 0.5, 1, 3, 6, 12, and 24 of culture. Data are shown as the mean ± SEM, n = 3 independent experiments. *P < 0.05, **P < 0.01, TF stimulation vs. control.
Mentions: In both 6% and 18% TF stimulation, the mRNA expression of Runx2 gradually increased through hour 3 of culture and decreased by hour 24 (Fig. 2a). The expression of Runx2 at 6% and 18% TF stimulation was significantly increased by 1.4–3.6 and 1.2–2.5-fold compared to control, respectively, at hours 0.25, 0.5, 1, 3, 6, 12, and/or 24 of culture.

Bottom Line: TF significantly increased extracellular ATP release compared to control.Six percent TF had maximum effect on ATP release compared to 18% TF and control.A438079 blocked the stimulatory effect of 6% TF on the expression of Runx2, Osterix and ECMPs, ALP activity, and calcium content in ECM.

View Article: PubMed Central - PubMed

Affiliation: Nihon University Graduate School of Dentistry, Tokyo, Japan.

Show MeSH
Related in: MedlinePlus