Tension force-induced ATP promotes osteogenesis through P2X7 receptor in osteoblasts.
Bottom Line: TF significantly increased extracellular ATP release compared to control.Six percent TF had maximum effect on ATP release compared to 18% TF and control.A438079 blocked the stimulatory effect of 6% TF on the expression of Runx2, Osterix and ECMPs, ALP activity, and calcium content in ECM.
Affiliation: Nihon University Graduate School of Dentistry, Tokyo, Japan.Show MeSH
Related in: MedlinePlus
Mentions: In both 6% and 18% TF stimulation, the mRNA expression of Runx2 gradually increased through hour 3 of culture and decreased by hour 24 (Fig. 2a). The expression of Runx2 at 6% and 18% TF stimulation was significantly increased by 1.4–3.6 and 1.2–2.5-fold compared to control, respectively, at hours 0.25, 0.5, 1, 3, 6, 12, and/or 24 of culture.
Affiliation: Nihon University Graduate School of Dentistry, Tokyo, Japan.