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Tension force-induced ATP promotes osteogenesis through P2X7 receptor in osteoblasts.

Kariya T, Tanabe N, Shionome C, Manaka S, Kawato T, Zhao N, Maeno M, Suzuki N, Shimizu N - J. Cell. Biochem. (2015)

Bottom Line: TF significantly increased extracellular ATP release compared to control.Six percent TF had maximum effect on ATP release compared to 18% TF and control.A438079 blocked the stimulatory effect of 6% TF on the expression of Runx2, Osterix and ECMPs, ALP activity, and calcium content in ECM.

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Affiliation: Nihon University Graduate School of Dentistry, Tokyo, Japan.

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Time course of ATP release by TF stimulation. MC3T3-E1 cells were cultured with ATPase inhibitor ARL67156 (50 μM) for 30 min before TF stimulation and stimulated by 0% (control), 6%, or 18% TF for 0, 1, 5, 10, or 15 min. ATP release was determined by Luciferin-luciferase bioluminescence assay. Data are expressed as the mean ± standard error (SEM), n = 3 independent experiments. *P < 0.05, **P < 0.01, TF stimulation vs. control.
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fig01: Time course of ATP release by TF stimulation. MC3T3-E1 cells were cultured with ATPase inhibitor ARL67156 (50 μM) for 30 min before TF stimulation and stimulated by 0% (control), 6%, or 18% TF for 0, 1, 5, 10, or 15 min. ATP release was determined by Luciferin-luciferase bioluminescence assay. Data are expressed as the mean ± standard error (SEM), n = 3 independent experiments. *P < 0.05, **P < 0.01, TF stimulation vs. control.

Mentions: We investigated the effect of TF stimulation on extracellular ATP release in osteoblasts. Both 6% and 18% TF stimulation enhanced extracellular ATP release in the culture media compared to control at 1 min, respectively. Six percent TF enhanced the extracellular ATP release compared to 18% TF (Fig. 1).


Tension force-induced ATP promotes osteogenesis through P2X7 receptor in osteoblasts.

Kariya T, Tanabe N, Shionome C, Manaka S, Kawato T, Zhao N, Maeno M, Suzuki N, Shimizu N - J. Cell. Biochem. (2015)

Time course of ATP release by TF stimulation. MC3T3-E1 cells were cultured with ATPase inhibitor ARL67156 (50 μM) for 30 min before TF stimulation and stimulated by 0% (control), 6%, or 18% TF for 0, 1, 5, 10, or 15 min. ATP release was determined by Luciferin-luciferase bioluminescence assay. Data are expressed as the mean ± standard error (SEM), n = 3 independent experiments. *P < 0.05, **P < 0.01, TF stimulation vs. control.
© Copyright Policy - open-access
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC4263259&req=5

fig01: Time course of ATP release by TF stimulation. MC3T3-E1 cells were cultured with ATPase inhibitor ARL67156 (50 μM) for 30 min before TF stimulation and stimulated by 0% (control), 6%, or 18% TF for 0, 1, 5, 10, or 15 min. ATP release was determined by Luciferin-luciferase bioluminescence assay. Data are expressed as the mean ± standard error (SEM), n = 3 independent experiments. *P < 0.05, **P < 0.01, TF stimulation vs. control.
Mentions: We investigated the effect of TF stimulation on extracellular ATP release in osteoblasts. Both 6% and 18% TF stimulation enhanced extracellular ATP release in the culture media compared to control at 1 min, respectively. Six percent TF enhanced the extracellular ATP release compared to 18% TF (Fig. 1).

Bottom Line: TF significantly increased extracellular ATP release compared to control.Six percent TF had maximum effect on ATP release compared to 18% TF and control.A438079 blocked the stimulatory effect of 6% TF on the expression of Runx2, Osterix and ECMPs, ALP activity, and calcium content in ECM.

View Article: PubMed Central - PubMed

Affiliation: Nihon University Graduate School of Dentistry, Tokyo, Japan.

Show MeSH
Related in: MedlinePlus