Beta-Adrenoceptor Activation Reduces Both Dermal Microvascular Endothelial Cell Migration via a cAMP-Dependent Mechanism and Wound Angiogenesis.
Bottom Line: Beta-adrenoceptors (β-AR) are G protein-coupled receptors (GPCRs) expressed on all skin cell-types.In this study, a range of in vitro assays (single cell migration, scratch wound healing, ELISAs for angiogenic growth factors and tubule formation) were performed with human dermal microvascular endothelial cells (HDMEC) to investigate and dissect mechanisms underpinning β-AR-mediated modulation of angiogenesis in chick chorioallantoic membranes (CAM) and murine excisional skin wounds. β-AR activation reduced HDMEC migration via cyclic adenosine monophosphate (cAMP)-dependent and protein kinase A (PKA)-independent mechanisms as demonstrated through use of an EPAC agonist that auto-inhibited the cAMP-mediated β-AR transduced reduction in HDMEC motility; a PKA inhibitor was, conversely, ineffective.In more complex environments, β-AR activation delayed HDMEC tubule formation and decreased angiogenesis both in the CAM assay and in murine excisional skin wounds in vivo. β-AR activation reduced HDMEC function in vitro and angiogenesis in vivo; therefore, β-AR agonists could be promising anti-angiogenic modulators in skin.
Affiliation: Department of Cell Physiology and Pharmacology, University of Leicester, Leicester, UK.Show MeSH
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Mentions: ECs undergo numerous physiological processes that contribute to angiogenesis, these include: invasion, alignment, elongation and apoptosis in addition to migration and proliferation (Bauer et al., 2005; Eming et al., 2007). To explore HDMEC physiological processes in a more complex environment, HDMECs were cultured on top of BME for 24 h in the presence or absence of Iso. HDMECs formed tubule-like structures after 6 h (Figs. 6A and B). To quantitate HDMEC tubule development, the number of tubule-like structures were counted. After 6 h, β-AR activation significantly delayed the formation of tubule-like structures by 14% (Fig. 6B).
Affiliation: Department of Cell Physiology and Pharmacology, University of Leicester, Leicester, UK.