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Determining the fibrillar orientation of bast fibres with polarized light microscopy: the modified Herzog test (red plate test) explained.

Haugan E, Holst B - J Microsc (2013)

Bottom Line: The test has the reputation for never producing false results, but also for occasionally not working.However, so far, no proper justification has been provided in the literature that the 'no false results' assumption is really correct and it has also not been clear up till now, why the method sometimes does not work.We also provide an explanation for why the Herzog test sometimes does not work: According to our model, the Herzog test will not work if none of the three distinct layers in the secondary cell wall is significantly thicker than the others.

View Article: PubMed Central - PubMed

Affiliation: Department of Physics and Technology, University of Bergen, Bergen, Norway.

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The modified Herzog test performed on cotton. Cotton is not a bast fibre with well-defined fibrillar orientation hence it will normally not be possible to bring it to extinction and it will show a rapid colour change along the fibre, regardless of the orientation angle. In some cases it may appear as if there is no colour change at all with orientation angle.
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fig13: The modified Herzog test performed on cotton. Cotton is not a bast fibre with well-defined fibrillar orientation hence it will normally not be possible to bring it to extinction and it will show a rapid colour change along the fibre, regardless of the orientation angle. In some cases it may appear as if there is no colour change at all with orientation angle.


Determining the fibrillar orientation of bast fibres with polarized light microscopy: the modified Herzog test (red plate test) explained.

Haugan E, Holst B - J Microsc (2013)

The modified Herzog test performed on cotton. Cotton is not a bast fibre with well-defined fibrillar orientation hence it will normally not be possible to bring it to extinction and it will show a rapid colour change along the fibre, regardless of the orientation angle. In some cases it may appear as if there is no colour change at all with orientation angle.
© Copyright Policy - open-access
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC4263192&req=5

fig13: The modified Herzog test performed on cotton. Cotton is not a bast fibre with well-defined fibrillar orientation hence it will normally not be possible to bring it to extinction and it will show a rapid colour change along the fibre, regardless of the orientation angle. In some cases it may appear as if there is no colour change at all with orientation angle.
Bottom Line: The test has the reputation for never producing false results, but also for occasionally not working.However, so far, no proper justification has been provided in the literature that the 'no false results' assumption is really correct and it has also not been clear up till now, why the method sometimes does not work.We also provide an explanation for why the Herzog test sometimes does not work: According to our model, the Herzog test will not work if none of the three distinct layers in the secondary cell wall is significantly thicker than the others.

View Article: PubMed Central - PubMed

Affiliation: Department of Physics and Technology, University of Bergen, Bergen, Norway.

Show MeSH