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Skeletal trauma generates systemic BMP2 activation that is temporally related to the mobilization of CD73+ cells.

Marsell R, Steen B, Bais MV, Mortlock DP, Einhorn TA, Gerstenfeld LC - J. Orthop. Res. (2013)

Bottom Line: Stem cell mobilization was analyzed by FACS analysis using CD73, a marker associated with bone marrow stromal stem cells.A ∼20% increase of CD73 positive cells was seen in the peripheral blood 2 days after reaming.These data showed that traumatic bone injury caused a systemic induction of BMP2 expression and that this increase is correlated with the mobilization of CD73 positive cells.

View Article: PubMed Central - PubMed

Affiliation: Department of Orthopaedic Surgery, Boston University Medical Center, 715 Albany Street, R-205, Boston, 02118, Massachusetts.

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Systemic mobilization of CD73 and CXCR4 positive cells after surgery FACS analysis of CD73 and CXCR4 positive cells in the peripheral blood after reaming compared to naïve controls. Statistically significant increases were found for CD73 and CXCR4 when measured individually, as well as that population of cells that co-expressed both markers (*p ≤ 0.05, ***p ≤ 0.001).
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fig04: Systemic mobilization of CD73 and CXCR4 positive cells after surgery FACS analysis of CD73 and CXCR4 positive cells in the peripheral blood after reaming compared to naïve controls. Statistically significant increases were found for CD73 and CXCR4 when measured individually, as well as that population of cells that co-expressed both markers (*p ≤ 0.05, ***p ≤ 0.001).

Mentions: The reciprocal relationship for the percentages of CD73 positive cells in the reamed and uninjured bones suggested that this population of cells might be recruited from the uninjured bone to the site of injury. In order to determine if reaming mobilized this population of cells into the circulation, the percentage of cells that sorted on CXCR4 or CD73 were next examined in the peripheral blood (Fig. 4). These results showed that cells positive for these two surface markers were indeed elevated in peripheral blood after injury consistent with prior results showing that CD73 and CXCR4 cells are mobilized into the circulation after fracture.17


Skeletal trauma generates systemic BMP2 activation that is temporally related to the mobilization of CD73+ cells.

Marsell R, Steen B, Bais MV, Mortlock DP, Einhorn TA, Gerstenfeld LC - J. Orthop. Res. (2013)

Systemic mobilization of CD73 and CXCR4 positive cells after surgery FACS analysis of CD73 and CXCR4 positive cells in the peripheral blood after reaming compared to naïve controls. Statistically significant increases were found for CD73 and CXCR4 when measured individually, as well as that population of cells that co-expressed both markers (*p ≤ 0.05, ***p ≤ 0.001).
© Copyright Policy - open-access
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC4263190&req=5

fig04: Systemic mobilization of CD73 and CXCR4 positive cells after surgery FACS analysis of CD73 and CXCR4 positive cells in the peripheral blood after reaming compared to naïve controls. Statistically significant increases were found for CD73 and CXCR4 when measured individually, as well as that population of cells that co-expressed both markers (*p ≤ 0.05, ***p ≤ 0.001).
Mentions: The reciprocal relationship for the percentages of CD73 positive cells in the reamed and uninjured bones suggested that this population of cells might be recruited from the uninjured bone to the site of injury. In order to determine if reaming mobilized this population of cells into the circulation, the percentage of cells that sorted on CXCR4 or CD73 were next examined in the peripheral blood (Fig. 4). These results showed that cells positive for these two surface markers were indeed elevated in peripheral blood after injury consistent with prior results showing that CD73 and CXCR4 cells are mobilized into the circulation after fracture.17

Bottom Line: Stem cell mobilization was analyzed by FACS analysis using CD73, a marker associated with bone marrow stromal stem cells.A ∼20% increase of CD73 positive cells was seen in the peripheral blood 2 days after reaming.These data showed that traumatic bone injury caused a systemic induction of BMP2 expression and that this increase is correlated with the mobilization of CD73 positive cells.

View Article: PubMed Central - PubMed

Affiliation: Department of Orthopaedic Surgery, Boston University Medical Center, 715 Albany Street, R-205, Boston, 02118, Massachusetts.

Show MeSH
Related in: MedlinePlus