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Development of antimicrobial biomaterials produced from chitin-nanofiber sheet/silver nanoparticle composites.

Nguyen VQ, Ishihara M, Kinoda J, Hattori H, Nakamura S, Ono T, Miyahira Y, Matsui T - J Nanobiotechnology (2014)

Bottom Line: CNFSs were immersed in suspensions of Ag NPs (5.17 ± 1.9 nm in diameter; mean ± SD) for 30 min at room temperature to produce CNFS/Ag NPs.Ultrathin sectioning of bacterial cells also was carried out to observe the bactericidal mechanism of Ag NPs.The TEM images indicated that the Ag NPs are dispersed and tightly adsorbed onto CNFSs.

View Article: PubMed Central - PubMed

Affiliation: Faculty of System Design, Tokyo Metropolitan University, 6-6 Asahigaoka, Hino, Tokyo, 191-0065, Japan. imvinhs@yahoo.com.vn.

ABSTRACT

Background: Chitin nanofibers sheets (CNFSs) with nanoscale fiber-like surface structures are nontoxic and biodegradable biomaterials with large surface-to-mass ratio. CNFSs are widely applied as biomedical materials such as a functional wound dressing. This study aimed to develop antimicrobial biomaterials made up of CNFS-immobilized silver nanoparticles (CNFS/Ag NPs).

Materials and methods: CNFSs were immersed in suspensions of Ag NPs (5.17 ± 1.9 nm in diameter; mean ± SD) for 30 min at room temperature to produce CNFS/Ag NPs. CNFS/Ag NPs were characterized by transmission electron microscopy (TEM) and then tested for antimicrobial activities against Escherichia (E.) coli, Pseudomonas (P.) aeruginosa, and H1N1 influenza A virus, three pathogens that represent the most widespread infectious bacteria and viruses. Ultrathin sectioning of bacterial cells also was carried out to observe the bactericidal mechanism of Ag NPs.

Results: The TEM images indicated that the Ag NPs are dispersed and tightly adsorbed onto CNFSs. Although CNFSs alone have only weak antimicrobial activity, CNFS/Ag NPs showed much stronger antimicrobial properties against E. coli, P. aeruginosa, and influenza A virus, with the amount of immobilized Ag NPs onto CNFSs.

Conclusions: Our results suggest that CNFS/Ag NPs interacting with those microbes exhibit stronger antimicrobial activities, and that it is possible to apply CNFS/Ag NPs as anti-virus sheets as well as anti-infectious wound dressings.

No MeSH data available.


Related in: MedlinePlus

Antiviral activity of CNFS/Ag NPs against H1N1influenza A virus. The viruses after treated with CNFS/Ag NPs were grown and their titers were determined with MDCK cells. At concentration of Ag NPs of 8.5 μg/1 cm2 CNFS, there was a reduction of greater than 2 log10 (100-fold) corresponding to reduction of viral titers by an approximate 99%. Data are mean value ± standard deviation; n = 3; the asterisk (*) represents statistically significant difference (p <0.01) using two-sample t-test.
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Fig6: Antiviral activity of CNFS/Ag NPs against H1N1influenza A virus. The viruses after treated with CNFS/Ag NPs were grown and their titers were determined with MDCK cells. At concentration of Ag NPs of 8.5 μg/1 cm2 CNFS, there was a reduction of greater than 2 log10 (100-fold) corresponding to reduction of viral titers by an approximate 99%. Data are mean value ± standard deviation; n = 3; the asterisk (*) represents statistically significant difference (p <0.01) using two-sample t-test.

Mentions: In order to confirm the antiviral activity of CNFS/Ag NPs, the CNFSs carrying various amounts of immobilized Ag NPs were evaluated for antiviral activity for human influenza A virus (A/PR/8/34 (H1N1)). The high concentration Ag NPs immobilized on CNFS gave significant decreases of virus number in log 10 CFU/ml, while CNFS alone gave only a little decrease against influenza A virus. At concentration of Ag NPs of 8.5 μg/1 cm2 chitin sheet, there was a reduction of greater than 2 log10 (100-fold) corresponding to reduction of viral titers by approximate 99% (Figure 6). This mentions that the antiviral activities of the CNFS/Ag NPs sheet were due to the interaction between virions and Ag NPs. The viruses may be adsorbed and immobilized on the CNFS/Ag NPs sheet. Therefore, inceasing amount of nAg on the chitin sheet makes more viruses adsored and immobilized on the CNFS/Ag NPs sheet.Figure 6


Development of antimicrobial biomaterials produced from chitin-nanofiber sheet/silver nanoparticle composites.

Nguyen VQ, Ishihara M, Kinoda J, Hattori H, Nakamura S, Ono T, Miyahira Y, Matsui T - J Nanobiotechnology (2014)

Antiviral activity of CNFS/Ag NPs against H1N1influenza A virus. The viruses after treated with CNFS/Ag NPs were grown and their titers were determined with MDCK cells. At concentration of Ag NPs of 8.5 μg/1 cm2 CNFS, there was a reduction of greater than 2 log10 (100-fold) corresponding to reduction of viral titers by an approximate 99%. Data are mean value ± standard deviation; n = 3; the asterisk (*) represents statistically significant difference (p <0.01) using two-sample t-test.
© Copyright Policy - open-access
Related In: Results  -  Collection

License 1 - License 2
Show All Figures
getmorefigures.php?uid=PMC4263038&req=5

Fig6: Antiviral activity of CNFS/Ag NPs against H1N1influenza A virus. The viruses after treated with CNFS/Ag NPs were grown and their titers were determined with MDCK cells. At concentration of Ag NPs of 8.5 μg/1 cm2 CNFS, there was a reduction of greater than 2 log10 (100-fold) corresponding to reduction of viral titers by an approximate 99%. Data are mean value ± standard deviation; n = 3; the asterisk (*) represents statistically significant difference (p <0.01) using two-sample t-test.
Mentions: In order to confirm the antiviral activity of CNFS/Ag NPs, the CNFSs carrying various amounts of immobilized Ag NPs were evaluated for antiviral activity for human influenza A virus (A/PR/8/34 (H1N1)). The high concentration Ag NPs immobilized on CNFS gave significant decreases of virus number in log 10 CFU/ml, while CNFS alone gave only a little decrease against influenza A virus. At concentration of Ag NPs of 8.5 μg/1 cm2 chitin sheet, there was a reduction of greater than 2 log10 (100-fold) corresponding to reduction of viral titers by approximate 99% (Figure 6). This mentions that the antiviral activities of the CNFS/Ag NPs sheet were due to the interaction between virions and Ag NPs. The viruses may be adsorbed and immobilized on the CNFS/Ag NPs sheet. Therefore, inceasing amount of nAg on the chitin sheet makes more viruses adsored and immobilized on the CNFS/Ag NPs sheet.Figure 6

Bottom Line: CNFSs were immersed in suspensions of Ag NPs (5.17 ± 1.9 nm in diameter; mean ± SD) for 30 min at room temperature to produce CNFS/Ag NPs.Ultrathin sectioning of bacterial cells also was carried out to observe the bactericidal mechanism of Ag NPs.The TEM images indicated that the Ag NPs are dispersed and tightly adsorbed onto CNFSs.

View Article: PubMed Central - PubMed

Affiliation: Faculty of System Design, Tokyo Metropolitan University, 6-6 Asahigaoka, Hino, Tokyo, 191-0065, Japan. imvinhs@yahoo.com.vn.

ABSTRACT

Background: Chitin nanofibers sheets (CNFSs) with nanoscale fiber-like surface structures are nontoxic and biodegradable biomaterials with large surface-to-mass ratio. CNFSs are widely applied as biomedical materials such as a functional wound dressing. This study aimed to develop antimicrobial biomaterials made up of CNFS-immobilized silver nanoparticles (CNFS/Ag NPs).

Materials and methods: CNFSs were immersed in suspensions of Ag NPs (5.17 ± 1.9 nm in diameter; mean ± SD) for 30 min at room temperature to produce CNFS/Ag NPs. CNFS/Ag NPs were characterized by transmission electron microscopy (TEM) and then tested for antimicrobial activities against Escherichia (E.) coli, Pseudomonas (P.) aeruginosa, and H1N1 influenza A virus, three pathogens that represent the most widespread infectious bacteria and viruses. Ultrathin sectioning of bacterial cells also was carried out to observe the bactericidal mechanism of Ag NPs.

Results: The TEM images indicated that the Ag NPs are dispersed and tightly adsorbed onto CNFSs. Although CNFSs alone have only weak antimicrobial activity, CNFS/Ag NPs showed much stronger antimicrobial properties against E. coli, P. aeruginosa, and influenza A virus, with the amount of immobilized Ag NPs onto CNFSs.

Conclusions: Our results suggest that CNFS/Ag NPs interacting with those microbes exhibit stronger antimicrobial activities, and that it is possible to apply CNFS/Ag NPs as anti-virus sheets as well as anti-infectious wound dressings.

No MeSH data available.


Related in: MedlinePlus