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Development of antimicrobial biomaterials produced from chitin-nanofiber sheet/silver nanoparticle composites.

Nguyen VQ, Ishihara M, Kinoda J, Hattori H, Nakamura S, Ono T, Miyahira Y, Matsui T - J Nanobiotechnology (2014)

Bottom Line: CNFSs were immersed in suspensions of Ag NPs (5.17 ± 1.9 nm in diameter; mean ± SD) for 30 min at room temperature to produce CNFS/Ag NPs.Ultrathin sectioning of bacterial cells also was carried out to observe the bactericidal mechanism of Ag NPs.The TEM images indicated that the Ag NPs are dispersed and tightly adsorbed onto CNFSs.

View Article: PubMed Central - PubMed

Affiliation: Faculty of System Design, Tokyo Metropolitan University, 6-6 Asahigaoka, Hino, Tokyo, 191-0065, Japan. imvinhs@yahoo.com.vn.

ABSTRACT

Background: Chitin nanofibers sheets (CNFSs) with nanoscale fiber-like surface structures are nontoxic and biodegradable biomaterials with large surface-to-mass ratio. CNFSs are widely applied as biomedical materials such as a functional wound dressing. This study aimed to develop antimicrobial biomaterials made up of CNFS-immobilized silver nanoparticles (CNFS/Ag NPs).

Materials and methods: CNFSs were immersed in suspensions of Ag NPs (5.17 ± 1.9 nm in diameter; mean ± SD) for 30 min at room temperature to produce CNFS/Ag NPs. CNFS/Ag NPs were characterized by transmission electron microscopy (TEM) and then tested for antimicrobial activities against Escherichia (E.) coli, Pseudomonas (P.) aeruginosa, and H1N1 influenza A virus, three pathogens that represent the most widespread infectious bacteria and viruses. Ultrathin sectioning of bacterial cells also was carried out to observe the bactericidal mechanism of Ag NPs.

Results: The TEM images indicated that the Ag NPs are dispersed and tightly adsorbed onto CNFSs. Although CNFSs alone have only weak antimicrobial activity, CNFS/Ag NPs showed much stronger antimicrobial properties against E. coli, P. aeruginosa, and influenza A virus, with the amount of immobilized Ag NPs onto CNFSs.

Conclusions: Our results suggest that CNFS/Ag NPs interacting with those microbes exhibit stronger antimicrobial activities, and that it is possible to apply CNFS/Ag NPs as anti-virus sheets as well as anti-infectious wound dressings.

No MeSH data available.


Related in: MedlinePlus

Bactericidal activities of CNFS/Ag NPs againstE. coli(A),andP. aeruginosa(B) at different concentration of Ag NPs. Data are mean value ± standard deviation (n = 6); the asterisk (*) indicates a statistically significant difference (p <0.01) using two-sample t-test.
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Fig5: Bactericidal activities of CNFS/Ag NPs againstE. coli(A),andP. aeruginosa(B) at different concentration of Ag NPs. Data are mean value ± standard deviation (n = 6); the asterisk (*) indicates a statistically significant difference (p <0.01) using two-sample t-test.

Mentions: Bactericidal tests of CNFS/Ag NPs were performed against E. coli and P. aeruginosa by counting the viable bacterial colonies after treatment with different concentrations of Ag NPs immobilized on CNFS (2.3, 3.8, 8.5 μg/1 cm2 CNFS). Samples of E. coli were completely eradicated when exposed to CNFS contained 8.5 μg/ml of Ag NPs. The high concentration Ag NPs immobilized on CNFS gave significant decreases of cell number in log 10 CFU/ml, while CNFS alone gave only a little decrease against E. coli and P. aeruginosa. Thus, the bactericidal activity of CNFS/Ag NPs increased with increased Ag NP loading (Figure 5).Figure 5


Development of antimicrobial biomaterials produced from chitin-nanofiber sheet/silver nanoparticle composites.

Nguyen VQ, Ishihara M, Kinoda J, Hattori H, Nakamura S, Ono T, Miyahira Y, Matsui T - J Nanobiotechnology (2014)

Bactericidal activities of CNFS/Ag NPs againstE. coli(A),andP. aeruginosa(B) at different concentration of Ag NPs. Data are mean value ± standard deviation (n = 6); the asterisk (*) indicates a statistically significant difference (p <0.01) using two-sample t-test.
© Copyright Policy - open-access
Related In: Results  -  Collection

License 1 - License 2
Show All Figures
getmorefigures.php?uid=PMC4263038&req=5

Fig5: Bactericidal activities of CNFS/Ag NPs againstE. coli(A),andP. aeruginosa(B) at different concentration of Ag NPs. Data are mean value ± standard deviation (n = 6); the asterisk (*) indicates a statistically significant difference (p <0.01) using two-sample t-test.
Mentions: Bactericidal tests of CNFS/Ag NPs were performed against E. coli and P. aeruginosa by counting the viable bacterial colonies after treatment with different concentrations of Ag NPs immobilized on CNFS (2.3, 3.8, 8.5 μg/1 cm2 CNFS). Samples of E. coli were completely eradicated when exposed to CNFS contained 8.5 μg/ml of Ag NPs. The high concentration Ag NPs immobilized on CNFS gave significant decreases of cell number in log 10 CFU/ml, while CNFS alone gave only a little decrease against E. coli and P. aeruginosa. Thus, the bactericidal activity of CNFS/Ag NPs increased with increased Ag NP loading (Figure 5).Figure 5

Bottom Line: CNFSs were immersed in suspensions of Ag NPs (5.17 ± 1.9 nm in diameter; mean ± SD) for 30 min at room temperature to produce CNFS/Ag NPs.Ultrathin sectioning of bacterial cells also was carried out to observe the bactericidal mechanism of Ag NPs.The TEM images indicated that the Ag NPs are dispersed and tightly adsorbed onto CNFSs.

View Article: PubMed Central - PubMed

Affiliation: Faculty of System Design, Tokyo Metropolitan University, 6-6 Asahigaoka, Hino, Tokyo, 191-0065, Japan. imvinhs@yahoo.com.vn.

ABSTRACT

Background: Chitin nanofibers sheets (CNFSs) with nanoscale fiber-like surface structures are nontoxic and biodegradable biomaterials with large surface-to-mass ratio. CNFSs are widely applied as biomedical materials such as a functional wound dressing. This study aimed to develop antimicrobial biomaterials made up of CNFS-immobilized silver nanoparticles (CNFS/Ag NPs).

Materials and methods: CNFSs were immersed in suspensions of Ag NPs (5.17 ± 1.9 nm in diameter; mean ± SD) for 30 min at room temperature to produce CNFS/Ag NPs. CNFS/Ag NPs were characterized by transmission electron microscopy (TEM) and then tested for antimicrobial activities against Escherichia (E.) coli, Pseudomonas (P.) aeruginosa, and H1N1 influenza A virus, three pathogens that represent the most widespread infectious bacteria and viruses. Ultrathin sectioning of bacterial cells also was carried out to observe the bactericidal mechanism of Ag NPs.

Results: The TEM images indicated that the Ag NPs are dispersed and tightly adsorbed onto CNFSs. Although CNFSs alone have only weak antimicrobial activity, CNFS/Ag NPs showed much stronger antimicrobial properties against E. coli, P. aeruginosa, and influenza A virus, with the amount of immobilized Ag NPs onto CNFSs.

Conclusions: Our results suggest that CNFS/Ag NPs interacting with those microbes exhibit stronger antimicrobial activities, and that it is possible to apply CNFS/Ag NPs as anti-virus sheets as well as anti-infectious wound dressings.

No MeSH data available.


Related in: MedlinePlus