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Development of antimicrobial biomaterials produced from chitin-nanofiber sheet/silver nanoparticle composites.

Nguyen VQ, Ishihara M, Kinoda J, Hattori H, Nakamura S, Ono T, Miyahira Y, Matsui T - J Nanobiotechnology (2014)

Bottom Line: CNFSs were immersed in suspensions of Ag NPs (5.17 ± 1.9 nm in diameter; mean ± SD) for 30 min at room temperature to produce CNFS/Ag NPs.Ultrathin sectioning of bacterial cells also was carried out to observe the bactericidal mechanism of Ag NPs.The TEM images indicated that the Ag NPs are dispersed and tightly adsorbed onto CNFSs.

View Article: PubMed Central - PubMed

Affiliation: Faculty of System Design, Tokyo Metropolitan University, 6-6 Asahigaoka, Hino, Tokyo, 191-0065, Japan. imvinhs@yahoo.com.vn.

ABSTRACT

Background: Chitin nanofibers sheets (CNFSs) with nanoscale fiber-like surface structures are nontoxic and biodegradable biomaterials with large surface-to-mass ratio. CNFSs are widely applied as biomedical materials such as a functional wound dressing. This study aimed to develop antimicrobial biomaterials made up of CNFS-immobilized silver nanoparticles (CNFS/Ag NPs).

Materials and methods: CNFSs were immersed in suspensions of Ag NPs (5.17 ± 1.9 nm in diameter; mean ± SD) for 30 min at room temperature to produce CNFS/Ag NPs. CNFS/Ag NPs were characterized by transmission electron microscopy (TEM) and then tested for antimicrobial activities against Escherichia (E.) coli, Pseudomonas (P.) aeruginosa, and H1N1 influenza A virus, three pathogens that represent the most widespread infectious bacteria and viruses. Ultrathin sectioning of bacterial cells also was carried out to observe the bactericidal mechanism of Ag NPs.

Results: The TEM images indicated that the Ag NPs are dispersed and tightly adsorbed onto CNFSs. Although CNFSs alone have only weak antimicrobial activity, CNFS/Ag NPs showed much stronger antimicrobial properties against E. coli, P. aeruginosa, and influenza A virus, with the amount of immobilized Ag NPs onto CNFSs.

Conclusions: Our results suggest that CNFS/Ag NPs interacting with those microbes exhibit stronger antimicrobial activities, and that it is possible to apply CNFS/Ag NPs as anti-virus sheets as well as anti-infectious wound dressings.

No MeSH data available.


Related in: MedlinePlus

Antimicrobial activity of CNFS/Ag NPs againstE.coli(A); CNFS alone againstE.coli(B); CNFS/Ag NPs againstP.aeruginosa(C); and CNFS alone againstP.aeruginosa(D). The CNFS/Ag NPs showed inhibition zone of bacterial growth as shown with red circles (A and C), although the CNFS alone exhibited no detectable inhibitory activity against either bacterial species (B and D).
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Fig4: Antimicrobial activity of CNFS/Ag NPs againstE.coli(A); CNFS alone againstE.coli(B); CNFS/Ag NPs againstP.aeruginosa(C); and CNFS alone againstP.aeruginosa(D). The CNFS/Ag NPs showed inhibition zone of bacterial growth as shown with red circles (A and C), although the CNFS alone exhibited no detectable inhibitory activity against either bacterial species (B and D).

Mentions: To establish the bactericidal properties of CNFS/Ag NPs, the sheet were tested with two infectious bacteria: E. coli and P. aeruginosa. The inhibition zone of bacterial growth around CNFS/Ag NPs and CNFS alone against E. coli and P. aeruginosa are shown in Figure 4. There was no zone of growth inhibition around CNFS alone for either E. coli or P. aeruginosa. With CNFS/Ag NPs (8.5 μg/ml), there were clear zones of inhibition of ≈ 30 mm diameter (for E. coli) and ≈ 25 mm diameter (for P. aeruginosa) around CNFS/Ag NPs after 24 h incubation (Figure 4).Figure 4


Development of antimicrobial biomaterials produced from chitin-nanofiber sheet/silver nanoparticle composites.

Nguyen VQ, Ishihara M, Kinoda J, Hattori H, Nakamura S, Ono T, Miyahira Y, Matsui T - J Nanobiotechnology (2014)

Antimicrobial activity of CNFS/Ag NPs againstE.coli(A); CNFS alone againstE.coli(B); CNFS/Ag NPs againstP.aeruginosa(C); and CNFS alone againstP.aeruginosa(D). The CNFS/Ag NPs showed inhibition zone of bacterial growth as shown with red circles (A and C), although the CNFS alone exhibited no detectable inhibitory activity against either bacterial species (B and D).
© Copyright Policy - open-access
Related In: Results  -  Collection

License 1 - License 2
Show All Figures
getmorefigures.php?uid=PMC4263038&req=5

Fig4: Antimicrobial activity of CNFS/Ag NPs againstE.coli(A); CNFS alone againstE.coli(B); CNFS/Ag NPs againstP.aeruginosa(C); and CNFS alone againstP.aeruginosa(D). The CNFS/Ag NPs showed inhibition zone of bacterial growth as shown with red circles (A and C), although the CNFS alone exhibited no detectable inhibitory activity against either bacterial species (B and D).
Mentions: To establish the bactericidal properties of CNFS/Ag NPs, the sheet were tested with two infectious bacteria: E. coli and P. aeruginosa. The inhibition zone of bacterial growth around CNFS/Ag NPs and CNFS alone against E. coli and P. aeruginosa are shown in Figure 4. There was no zone of growth inhibition around CNFS alone for either E. coli or P. aeruginosa. With CNFS/Ag NPs (8.5 μg/ml), there were clear zones of inhibition of ≈ 30 mm diameter (for E. coli) and ≈ 25 mm diameter (for P. aeruginosa) around CNFS/Ag NPs after 24 h incubation (Figure 4).Figure 4

Bottom Line: CNFSs were immersed in suspensions of Ag NPs (5.17 ± 1.9 nm in diameter; mean ± SD) for 30 min at room temperature to produce CNFS/Ag NPs.Ultrathin sectioning of bacterial cells also was carried out to observe the bactericidal mechanism of Ag NPs.The TEM images indicated that the Ag NPs are dispersed and tightly adsorbed onto CNFSs.

View Article: PubMed Central - PubMed

Affiliation: Faculty of System Design, Tokyo Metropolitan University, 6-6 Asahigaoka, Hino, Tokyo, 191-0065, Japan. imvinhs@yahoo.com.vn.

ABSTRACT

Background: Chitin nanofibers sheets (CNFSs) with nanoscale fiber-like surface structures are nontoxic and biodegradable biomaterials with large surface-to-mass ratio. CNFSs are widely applied as biomedical materials such as a functional wound dressing. This study aimed to develop antimicrobial biomaterials made up of CNFS-immobilized silver nanoparticles (CNFS/Ag NPs).

Materials and methods: CNFSs were immersed in suspensions of Ag NPs (5.17 ± 1.9 nm in diameter; mean ± SD) for 30 min at room temperature to produce CNFS/Ag NPs. CNFS/Ag NPs were characterized by transmission electron microscopy (TEM) and then tested for antimicrobial activities against Escherichia (E.) coli, Pseudomonas (P.) aeruginosa, and H1N1 influenza A virus, three pathogens that represent the most widespread infectious bacteria and viruses. Ultrathin sectioning of bacterial cells also was carried out to observe the bactericidal mechanism of Ag NPs.

Results: The TEM images indicated that the Ag NPs are dispersed and tightly adsorbed onto CNFSs. Although CNFSs alone have only weak antimicrobial activity, CNFS/Ag NPs showed much stronger antimicrobial properties against E. coli, P. aeruginosa, and influenza A virus, with the amount of immobilized Ag NPs onto CNFSs.

Conclusions: Our results suggest that CNFS/Ag NPs interacting with those microbes exhibit stronger antimicrobial activities, and that it is possible to apply CNFS/Ag NPs as anti-virus sheets as well as anti-infectious wound dressings.

No MeSH data available.


Related in: MedlinePlus