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Identification of a new tospovirus causing necrotic ringspot on tomato in China.

Yin Y, Zheng K, Dong J, Fang Q, Wu S, Wang L, Zhang Z - Virol. J. (2014)

Bottom Line: Emerging tospoviruses cause significant yield losses and quality reduction in vegetables, ornamentals, and legumes throughout the world.Tomato fruits displaying necrotic and concentric ringspot symptoms were found in Guizhou province of southwest China.This finding suggests that a detailed survey in China is warranted to further understand the occurrence and distribution of tospoviruses.

View Article: PubMed Central - PubMed

Affiliation: Yunnan Key Laboratory of Agricultural Biotechnology, Key Lab of Southwestern Crop Gene Resources and Germplasm Innovation, Biotechnology and Germplasm Resources Institute, Ministry of Agriculture, Yunnan Academy of Agricultural Sciences, Kunming, 650223, China. yinyy0324@163.com.

ABSTRACT

Background: Emerging tospoviruses cause significant yield losses and quality reduction in vegetables, ornamentals, and legumes throughout the world. So far, eight tospoviruses were reported in China. Tomato fruits displaying necrotic and concentric ringspot symptoms were found in Guizhou province of southwest China.

Finding: ELISA experiments showed that crude saps of the diseased tomato fruit samples reacted with antiserum against Tomato zonate spot virus (TZSV). Electron microscopy detected presence of quasi-spherical, enveloped particles of 80-100 nm in such saps. The putative virus isolate was designated 2009-GZT. Mechanical back-inoculation showed that 2009-GZT could infect systemically some solanaceous crop and non-crop plants including Capiscum annuum, Datura stramonium, Nicotiana benthamiana, N. rustica, N. tabacum and Solanum lycopersicum. The 3012 nt full-length sequence of 2009-GZT S RNA shared 68.2% nt identity with that of Calla lily chlorotic spot virus (CCSV), the highest among all compared viruses. This RNA was predicted to encode a non-structural protein (NSs) (459 aa, 51.7 kDa) and a nucleocapsid protein (N) (278 aa, 30.3 kDa). The N protein shared 85.8% amino acid identity with that of CCSV. The NSs protein shared 82.7% amino acid identity with that of Tomato zonate spot virus(TZSV).

Conclusion: Our results indicate that the isolate 2009-GZT is a new species of Tospovirus, which is named Tomato necrotic spot virus (TNSV). This finding suggests that a detailed survey in China is warranted to further understand the occurrence and distribution of tospoviruses.

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Symptoms and virus particles of Tomato necrotic spot virus. A. Necrotic ringspot on tomato fruits. B. Electron micrograph of Tomato necrotic spot virus particles (Bar = 100 nm).
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Fig1: Symptoms and virus particles of Tomato necrotic spot virus. A. Necrotic ringspot on tomato fruits. B. Electron micrograph of Tomato necrotic spot virus particles (Bar = 100 nm).

Mentions: Tomato fruit samples showing necrotic ringspot and concentric ringspot symptoms were collected from Wengan County, Guizhou province, China in 2009 (Figure 1A). These samples were tested by ELISA with antisera against GRSV/TCSV, INSV, IYSV, TSWV, and TZSV, respectively. The symptomatic samples reacted with the antiserum against TZSV N protein. The virus isolate, 2009-GZT, was obtained via three consecutive single lesion transfer in C. quinoa and in systemically infected N. rustica after mechanical inoculation. Further infection studies were used to determine the experimental host range of 2009-GZT. Systemic infection was observed in C. annuum, D. stramonium, N. benthamiana, N. debneyi, N. rustica, N. tabacum and S. lycopersicum, with the infected plants showing leaf chlorosis, necrosis, ringspot and/or fruit deformation. Local lesion infection was found in C. amaranticolor, C. quinoa and N. glutinosa. The virus did not infect Cucumis sativus (Table 1). ELISA showed that samples from all infected plants reacted positively with the TZSV-antiserum, but did not react with GRSV/TCSV, INSV, IYSV and TSWV antisera.Figure 1


Identification of a new tospovirus causing necrotic ringspot on tomato in China.

Yin Y, Zheng K, Dong J, Fang Q, Wu S, Wang L, Zhang Z - Virol. J. (2014)

Symptoms and virus particles of Tomato necrotic spot virus. A. Necrotic ringspot on tomato fruits. B. Electron micrograph of Tomato necrotic spot virus particles (Bar = 100 nm).
© Copyright Policy - open-access
Related In: Results  -  Collection

License 1 - License 2
Show All Figures
getmorefigures.php?uid=PMC4263035&req=5

Fig1: Symptoms and virus particles of Tomato necrotic spot virus. A. Necrotic ringspot on tomato fruits. B. Electron micrograph of Tomato necrotic spot virus particles (Bar = 100 nm).
Mentions: Tomato fruit samples showing necrotic ringspot and concentric ringspot symptoms were collected from Wengan County, Guizhou province, China in 2009 (Figure 1A). These samples were tested by ELISA with antisera against GRSV/TCSV, INSV, IYSV, TSWV, and TZSV, respectively. The symptomatic samples reacted with the antiserum against TZSV N protein. The virus isolate, 2009-GZT, was obtained via three consecutive single lesion transfer in C. quinoa and in systemically infected N. rustica after mechanical inoculation. Further infection studies were used to determine the experimental host range of 2009-GZT. Systemic infection was observed in C. annuum, D. stramonium, N. benthamiana, N. debneyi, N. rustica, N. tabacum and S. lycopersicum, with the infected plants showing leaf chlorosis, necrosis, ringspot and/or fruit deformation. Local lesion infection was found in C. amaranticolor, C. quinoa and N. glutinosa. The virus did not infect Cucumis sativus (Table 1). ELISA showed that samples from all infected plants reacted positively with the TZSV-antiserum, but did not react with GRSV/TCSV, INSV, IYSV and TSWV antisera.Figure 1

Bottom Line: Emerging tospoviruses cause significant yield losses and quality reduction in vegetables, ornamentals, and legumes throughout the world.Tomato fruits displaying necrotic and concentric ringspot symptoms were found in Guizhou province of southwest China.This finding suggests that a detailed survey in China is warranted to further understand the occurrence and distribution of tospoviruses.

View Article: PubMed Central - PubMed

Affiliation: Yunnan Key Laboratory of Agricultural Biotechnology, Key Lab of Southwestern Crop Gene Resources and Germplasm Innovation, Biotechnology and Germplasm Resources Institute, Ministry of Agriculture, Yunnan Academy of Agricultural Sciences, Kunming, 650223, China. yinyy0324@163.com.

ABSTRACT

Background: Emerging tospoviruses cause significant yield losses and quality reduction in vegetables, ornamentals, and legumes throughout the world. So far, eight tospoviruses were reported in China. Tomato fruits displaying necrotic and concentric ringspot symptoms were found in Guizhou province of southwest China.

Finding: ELISA experiments showed that crude saps of the diseased tomato fruit samples reacted with antiserum against Tomato zonate spot virus (TZSV). Electron microscopy detected presence of quasi-spherical, enveloped particles of 80-100 nm in such saps. The putative virus isolate was designated 2009-GZT. Mechanical back-inoculation showed that 2009-GZT could infect systemically some solanaceous crop and non-crop plants including Capiscum annuum, Datura stramonium, Nicotiana benthamiana, N. rustica, N. tabacum and Solanum lycopersicum. The 3012 nt full-length sequence of 2009-GZT S RNA shared 68.2% nt identity with that of Calla lily chlorotic spot virus (CCSV), the highest among all compared viruses. This RNA was predicted to encode a non-structural protein (NSs) (459 aa, 51.7 kDa) and a nucleocapsid protein (N) (278 aa, 30.3 kDa). The N protein shared 85.8% amino acid identity with that of CCSV. The NSs protein shared 82.7% amino acid identity with that of Tomato zonate spot virus(TZSV).

Conclusion: Our results indicate that the isolate 2009-GZT is a new species of Tospovirus, which is named Tomato necrotic spot virus (TNSV). This finding suggests that a detailed survey in China is warranted to further understand the occurrence and distribution of tospoviruses.

Show MeSH
Related in: MedlinePlus