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Distinct transcriptional profiles of ozone stress in soybean (Glycine max) flowers and pods.

Leisner CP, Ming R, Ainsworth EA - BMC Plant Biol. (2014)

Bottom Line: Current ground-level [O3] are estimated to reduce global soybean yields by 6% to 16%.Pod tissues responded to elevated [O3] by increasing expression of xyloglucan endotransglucosylase/hydrolase genes, which may be involved with increased pod dehiscence in elevated [O3].This study established that gene expression in reproductive tissues of soybean are impacted by elevated [O3], and flowers and pods have distinct transcriptomic responses to elevated [O3].

View Article: PubMed Central - PubMed

Affiliation: Department of Plant Biology, University of Illinois, Urbana-Champaign, Urbana, IL, 61801, USA. leisner1@illinois.edu.

ABSTRACT

Background: Tropospheric ozone (O3) is a secondary air pollutant and anthropogenic greenhouse gas. Concentrations of tropospheric O3 ([O3] have more than doubled since the Industrial Revolution, and are high enough to damage plant productivity. Soybean (Glycine max L. Merr.) is the world's most important legume crop and is sensitive to O3. Current ground-level [O3] are estimated to reduce global soybean yields by 6% to 16%. In order to understand transcriptional mechanisms of yield loss in soybean, we examined the transcriptome of soybean flower and pod tissues exposed to elevated [O3] using RNA-Sequencing.

Results: Elevated [O3] elicited a strong transcriptional response in flower and pod tissues, with increased expression of genes involved in signaling in both tissues. Flower tissues also responded to elevated [O3] by increasing expression of genes encoding matrix metalloproteinases (MMPs). MMPs are zinc- and calcium-dependent endopeptidases that have roles in programmed cell death, senescence and stress response in plants. Pod tissues responded to elevated [O3] by increasing expression of xyloglucan endotransglucosylase/hydrolase genes, which may be involved with increased pod dehiscence in elevated [O3].

Conclusions: This study established that gene expression in reproductive tissues of soybean are impacted by elevated [O3], and flowers and pods have distinct transcriptomic responses to elevated [O3].

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Venn diagram of differentially expressed genes in flower and pod tissues in response to elevated [O3]. Numbers of genes that were differentially expressed in response to elevated [O3] in flowers (green), pods (purple) and in both tissues (overlapping).
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Fig3: Venn diagram of differentially expressed genes in flower and pod tissues in response to elevated [O3]. Numbers of genes that were differentially expressed in response to elevated [O3] in flowers (green), pods (purple) and in both tissues (overlapping).

Mentions: Flower and pod development in soybean are sensitive to environmental stress [23,24,38,39], and elevated [O3] significantly impacted pod production, but not flower production (Figure 1). In order to identify the genetic mechanisms underpinning O3 response in soybean pods and flowers, the transcriptome of flower and pod tissues was compared using RNA-Sequencing (RNA-Seq). The global transcriptional analysis showed the magnitude of potential responses to elevated [O3] in flowers and pods was similar, with genes showing approximately the same range of both mean expression values in flowers and pods, and similar potential log fold change responses to elevated [O3] in the two tissues (Figure 2). However, more than three times as many genes were differentially expressed in flower tissue (4,595 genes) than in pod tissue (1,375 genes; Figure 3) in response to elevated [O3], and only 277 of those genes were differentially expressed in both flowers and pods (Figure 3).Figure 2


Distinct transcriptional profiles of ozone stress in soybean (Glycine max) flowers and pods.

Leisner CP, Ming R, Ainsworth EA - BMC Plant Biol. (2014)

Venn diagram of differentially expressed genes in flower and pod tissues in response to elevated [O3]. Numbers of genes that were differentially expressed in response to elevated [O3] in flowers (green), pods (purple) and in both tissues (overlapping).
© Copyright Policy - open-access
Related In: Results  -  Collection

License 1 - License 2
Show All Figures
getmorefigures.php?uid=PMC4263021&req=5

Fig3: Venn diagram of differentially expressed genes in flower and pod tissues in response to elevated [O3]. Numbers of genes that were differentially expressed in response to elevated [O3] in flowers (green), pods (purple) and in both tissues (overlapping).
Mentions: Flower and pod development in soybean are sensitive to environmental stress [23,24,38,39], and elevated [O3] significantly impacted pod production, but not flower production (Figure 1). In order to identify the genetic mechanisms underpinning O3 response in soybean pods and flowers, the transcriptome of flower and pod tissues was compared using RNA-Sequencing (RNA-Seq). The global transcriptional analysis showed the magnitude of potential responses to elevated [O3] in flowers and pods was similar, with genes showing approximately the same range of both mean expression values in flowers and pods, and similar potential log fold change responses to elevated [O3] in the two tissues (Figure 2). However, more than three times as many genes were differentially expressed in flower tissue (4,595 genes) than in pod tissue (1,375 genes; Figure 3) in response to elevated [O3], and only 277 of those genes were differentially expressed in both flowers and pods (Figure 3).Figure 2

Bottom Line: Current ground-level [O3] are estimated to reduce global soybean yields by 6% to 16%.Pod tissues responded to elevated [O3] by increasing expression of xyloglucan endotransglucosylase/hydrolase genes, which may be involved with increased pod dehiscence in elevated [O3].This study established that gene expression in reproductive tissues of soybean are impacted by elevated [O3], and flowers and pods have distinct transcriptomic responses to elevated [O3].

View Article: PubMed Central - PubMed

Affiliation: Department of Plant Biology, University of Illinois, Urbana-Champaign, Urbana, IL, 61801, USA. leisner1@illinois.edu.

ABSTRACT

Background: Tropospheric ozone (O3) is a secondary air pollutant and anthropogenic greenhouse gas. Concentrations of tropospheric O3 ([O3] have more than doubled since the Industrial Revolution, and are high enough to damage plant productivity. Soybean (Glycine max L. Merr.) is the world's most important legume crop and is sensitive to O3. Current ground-level [O3] are estimated to reduce global soybean yields by 6% to 16%. In order to understand transcriptional mechanisms of yield loss in soybean, we examined the transcriptome of soybean flower and pod tissues exposed to elevated [O3] using RNA-Sequencing.

Results: Elevated [O3] elicited a strong transcriptional response in flower and pod tissues, with increased expression of genes involved in signaling in both tissues. Flower tissues also responded to elevated [O3] by increasing expression of genes encoding matrix metalloproteinases (MMPs). MMPs are zinc- and calcium-dependent endopeptidases that have roles in programmed cell death, senescence and stress response in plants. Pod tissues responded to elevated [O3] by increasing expression of xyloglucan endotransglucosylase/hydrolase genes, which may be involved with increased pod dehiscence in elevated [O3].

Conclusions: This study established that gene expression in reproductive tissues of soybean are impacted by elevated [O3], and flowers and pods have distinct transcriptomic responses to elevated [O3].

Show MeSH
Related in: MedlinePlus