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Changes in responses of neurons in spinal and medullary subnucleus reticularis dorsalis to acupoint stimulation in rats with visceral hyperalgesia.

Yu LL, Li L, Rong PJ, Zhu B, Qin QG, Ben H, Huang GF - Evid Based Complement Alternat Med (2014)

Bottom Line: In medulla oblongata, EA-induced activation of SRD neurons further increased to 63.28 ± 15.96% (1.5 mA) (P < 0.001) and 25.02 ± 7.47% (6 mA) (P < 0.01) compared to that before CRD.Taken together, these data suggest that the viscerosomatic convergence-facilitation effect of WDR and SRD neurons may underlie the mechanism of acupoints sensitization.But the sensitizing effect of visceral nociception on WDR neurons is stronger than that on SRD neurons.

View Article: PubMed Central - PubMed

Affiliation: Department of Acupuncture and Moxibustion, Wuhan Hospital of Integrated Chinese and Western Medicine, 215 Zhongshan Road, Wuhan 430022, China ; Institute of Acupuncture and Moxibustion, China Academy of Chinese Medical Sciences, Beijing 100700, China.

ABSTRACT
The purpose of this study was to explore the mechanism of acupoints sensitization phenomenon at the spinal and medulla levels. Experiments were performed on adult male Sprague-Dawley rats and visceral noxious stimuli was generated by colorectal distension (CRD). The activities of wide dynamic range (WDR) and subnucleus reticularis dorsalis (SRD) neurons were recorded. The changes of the reactions of WDR and SRD neurons to electroacupuncture (EA) on acupoints of "Zusanli-Shangjuxu" before and after CRD stimulation were observed. The results showed that visceral nociception could facilitate the response of neurons to acupoints stimulation. In spinal dorsal horn, EA-induced activation of WDR neurons further increased to 106.84 ± 17.33% (1.5 mA) (P < 0.001) and 42.27 ± 13.10% (6 mA) (P < 0.01) compared to the neuronal responses before CRD. In medulla oblongata, EA-induced activation of SRD neurons further increased to 63.28 ± 15.96% (1.5 mA) (P < 0.001) and 25.02 ± 7.47% (6 mA) (P < 0.01) compared to that before CRD. Taken together, these data suggest that the viscerosomatic convergence-facilitation effect of WDR and SRD neurons may underlie the mechanism of acupoints sensitization. But the sensitizing effect of visceral nociception on WDR neurons is stronger than that on SRD neurons.

No MeSH data available.


Related in: MedlinePlus

The response patterns of SRD neurons to EA before and after CRD. (a) A neuron not activated by 1.5 mA before CRD but activated after CRD. (b) A neuron activated by 6 mA both before and after CRD but showing a stronger response to EA after CRD.
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fig7: The response patterns of SRD neurons to EA before and after CRD. (a) A neuron not activated by 1.5 mA before CRD but activated after CRD. (b) A neuron activated by 6 mA both before and after CRD but showing a stronger response to EA after CRD.

Mentions: Different intensities of EA were applied at acupoints of “Zusanli-Shangjuxu” before and after rats were given 80 mmHg CRD for 60 s. Figure 7 indicates that the responses of SRD neurons to EA before and after CRD were different. After CRD, the numbers of discharges of SRD neurons evoked by EA of the same intensity before CRD were also significantly increased (see Table 2).


Changes in responses of neurons in spinal and medullary subnucleus reticularis dorsalis to acupoint stimulation in rats with visceral hyperalgesia.

Yu LL, Li L, Rong PJ, Zhu B, Qin QG, Ben H, Huang GF - Evid Based Complement Alternat Med (2014)

The response patterns of SRD neurons to EA before and after CRD. (a) A neuron not activated by 1.5 mA before CRD but activated after CRD. (b) A neuron activated by 6 mA both before and after CRD but showing a stronger response to EA after CRD.
© Copyright Policy - open-access
Related In: Results  -  Collection

Show All Figures
getmorefigures.php?uid=PMC4262754&req=5

fig7: The response patterns of SRD neurons to EA before and after CRD. (a) A neuron not activated by 1.5 mA before CRD but activated after CRD. (b) A neuron activated by 6 mA both before and after CRD but showing a stronger response to EA after CRD.
Mentions: Different intensities of EA were applied at acupoints of “Zusanli-Shangjuxu” before and after rats were given 80 mmHg CRD for 60 s. Figure 7 indicates that the responses of SRD neurons to EA before and after CRD were different. After CRD, the numbers of discharges of SRD neurons evoked by EA of the same intensity before CRD were also significantly increased (see Table 2).

Bottom Line: In medulla oblongata, EA-induced activation of SRD neurons further increased to 63.28 ± 15.96% (1.5 mA) (P < 0.001) and 25.02 ± 7.47% (6 mA) (P < 0.01) compared to that before CRD.Taken together, these data suggest that the viscerosomatic convergence-facilitation effect of WDR and SRD neurons may underlie the mechanism of acupoints sensitization.But the sensitizing effect of visceral nociception on WDR neurons is stronger than that on SRD neurons.

View Article: PubMed Central - PubMed

Affiliation: Department of Acupuncture and Moxibustion, Wuhan Hospital of Integrated Chinese and Western Medicine, 215 Zhongshan Road, Wuhan 430022, China ; Institute of Acupuncture and Moxibustion, China Academy of Chinese Medical Sciences, Beijing 100700, China.

ABSTRACT
The purpose of this study was to explore the mechanism of acupoints sensitization phenomenon at the spinal and medulla levels. Experiments were performed on adult male Sprague-Dawley rats and visceral noxious stimuli was generated by colorectal distension (CRD). The activities of wide dynamic range (WDR) and subnucleus reticularis dorsalis (SRD) neurons were recorded. The changes of the reactions of WDR and SRD neurons to electroacupuncture (EA) on acupoints of "Zusanli-Shangjuxu" before and after CRD stimulation were observed. The results showed that visceral nociception could facilitate the response of neurons to acupoints stimulation. In spinal dorsal horn, EA-induced activation of WDR neurons further increased to 106.84 ± 17.33% (1.5 mA) (P < 0.001) and 42.27 ± 13.10% (6 mA) (P < 0.01) compared to the neuronal responses before CRD. In medulla oblongata, EA-induced activation of SRD neurons further increased to 63.28 ± 15.96% (1.5 mA) (P < 0.001) and 25.02 ± 7.47% (6 mA) (P < 0.01) compared to that before CRD. Taken together, these data suggest that the viscerosomatic convergence-facilitation effect of WDR and SRD neurons may underlie the mechanism of acupoints sensitization. But the sensitizing effect of visceral nociception on WDR neurons is stronger than that on SRD neurons.

No MeSH data available.


Related in: MedlinePlus