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Changes in responses of neurons in spinal and medullary subnucleus reticularis dorsalis to acupoint stimulation in rats with visceral hyperalgesia.

Yu LL, Li L, Rong PJ, Zhu B, Qin QG, Ben H, Huang GF - Evid Based Complement Alternat Med (2014)

Bottom Line: In medulla oblongata, EA-induced activation of SRD neurons further increased to 63.28 ± 15.96% (1.5 mA) (P < 0.001) and 25.02 ± 7.47% (6 mA) (P < 0.01) compared to that before CRD.Taken together, these data suggest that the viscerosomatic convergence-facilitation effect of WDR and SRD neurons may underlie the mechanism of acupoints sensitization.But the sensitizing effect of visceral nociception on WDR neurons is stronger than that on SRD neurons.

View Article: PubMed Central - PubMed

Affiliation: Department of Acupuncture and Moxibustion, Wuhan Hospital of Integrated Chinese and Western Medicine, 215 Zhongshan Road, Wuhan 430022, China ; Institute of Acupuncture and Moxibustion, China Academy of Chinese Medical Sciences, Beijing 100700, China.

ABSTRACT
The purpose of this study was to explore the mechanism of acupoints sensitization phenomenon at the spinal and medulla levels. Experiments were performed on adult male Sprague-Dawley rats and visceral noxious stimuli was generated by colorectal distension (CRD). The activities of wide dynamic range (WDR) and subnucleus reticularis dorsalis (SRD) neurons were recorded. The changes of the reactions of WDR and SRD neurons to electroacupuncture (EA) on acupoints of "Zusanli-Shangjuxu" before and after CRD stimulation were observed. The results showed that visceral nociception could facilitate the response of neurons to acupoints stimulation. In spinal dorsal horn, EA-induced activation of WDR neurons further increased to 106.84 ± 17.33% (1.5 mA) (P < 0.001) and 42.27 ± 13.10% (6 mA) (P < 0.01) compared to the neuronal responses before CRD. In medulla oblongata, EA-induced activation of SRD neurons further increased to 63.28 ± 15.96% (1.5 mA) (P < 0.001) and 25.02 ± 7.47% (6 mA) (P < 0.01) compared to that before CRD. Taken together, these data suggest that the viscerosomatic convergence-facilitation effect of WDR and SRD neurons may underlie the mechanism of acupoints sensitization. But the sensitizing effect of visceral nociception on WDR neurons is stronger than that on SRD neurons.

No MeSH data available.


Related in: MedlinePlus

(a) Positions of WDR neurons marked by pontamine sky blue in the spinal dorsal horn. (b) The skin receptive fields of WDR neurons.
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fig1: (a) Positions of WDR neurons marked by pontamine sky blue in the spinal dorsal horn. (b) The skin receptive fields of WDR neurons.

Mentions: 30 WDR neurons were well isolated from the background. Most of the WDR neurons were located in laminas IV and V, and a few in laminas I and VI of the gray matter marked by electrophoresis of the pontamine sky blue dye at the end of experiment (see Figure 1(a)). The skin receptive fields of the majority of the WDR neurons responding to somatic stimuli were distributed along the ipsilateral caudal parts of the body, including the scrotum, hip region, tail root, hind limb, and the hind paw (see Figure 1(b)).


Changes in responses of neurons in spinal and medullary subnucleus reticularis dorsalis to acupoint stimulation in rats with visceral hyperalgesia.

Yu LL, Li L, Rong PJ, Zhu B, Qin QG, Ben H, Huang GF - Evid Based Complement Alternat Med (2014)

(a) Positions of WDR neurons marked by pontamine sky blue in the spinal dorsal horn. (b) The skin receptive fields of WDR neurons.
© Copyright Policy - open-access
Related In: Results  -  Collection

Show All Figures
getmorefigures.php?uid=PMC4262754&req=5

fig1: (a) Positions of WDR neurons marked by pontamine sky blue in the spinal dorsal horn. (b) The skin receptive fields of WDR neurons.
Mentions: 30 WDR neurons were well isolated from the background. Most of the WDR neurons were located in laminas IV and V, and a few in laminas I and VI of the gray matter marked by electrophoresis of the pontamine sky blue dye at the end of experiment (see Figure 1(a)). The skin receptive fields of the majority of the WDR neurons responding to somatic stimuli were distributed along the ipsilateral caudal parts of the body, including the scrotum, hip region, tail root, hind limb, and the hind paw (see Figure 1(b)).

Bottom Line: In medulla oblongata, EA-induced activation of SRD neurons further increased to 63.28 ± 15.96% (1.5 mA) (P < 0.001) and 25.02 ± 7.47% (6 mA) (P < 0.01) compared to that before CRD.Taken together, these data suggest that the viscerosomatic convergence-facilitation effect of WDR and SRD neurons may underlie the mechanism of acupoints sensitization.But the sensitizing effect of visceral nociception on WDR neurons is stronger than that on SRD neurons.

View Article: PubMed Central - PubMed

Affiliation: Department of Acupuncture and Moxibustion, Wuhan Hospital of Integrated Chinese and Western Medicine, 215 Zhongshan Road, Wuhan 430022, China ; Institute of Acupuncture and Moxibustion, China Academy of Chinese Medical Sciences, Beijing 100700, China.

ABSTRACT
The purpose of this study was to explore the mechanism of acupoints sensitization phenomenon at the spinal and medulla levels. Experiments were performed on adult male Sprague-Dawley rats and visceral noxious stimuli was generated by colorectal distension (CRD). The activities of wide dynamic range (WDR) and subnucleus reticularis dorsalis (SRD) neurons were recorded. The changes of the reactions of WDR and SRD neurons to electroacupuncture (EA) on acupoints of "Zusanli-Shangjuxu" before and after CRD stimulation were observed. The results showed that visceral nociception could facilitate the response of neurons to acupoints stimulation. In spinal dorsal horn, EA-induced activation of WDR neurons further increased to 106.84 ± 17.33% (1.5 mA) (P < 0.001) and 42.27 ± 13.10% (6 mA) (P < 0.01) compared to the neuronal responses before CRD. In medulla oblongata, EA-induced activation of SRD neurons further increased to 63.28 ± 15.96% (1.5 mA) (P < 0.001) and 25.02 ± 7.47% (6 mA) (P < 0.01) compared to that before CRD. Taken together, these data suggest that the viscerosomatic convergence-facilitation effect of WDR and SRD neurons may underlie the mechanism of acupoints sensitization. But the sensitizing effect of visceral nociception on WDR neurons is stronger than that on SRD neurons.

No MeSH data available.


Related in: MedlinePlus