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Characterisation of immune cell function in fragment and full-length Huntington's disease mouse models.

Träger U, Andre R, Magnusson-Lind A, Miller JR, Connolly C, Weiss A, Grueninger S, Silajdžić E, Smith DL, Leavitt BR, Bates GP, Björkqvist M, Tabrizi SJ - Neurobiol. Dis. (2014)

Bottom Line: Inflammation is a growing area of research in neurodegeneration.However, bone marrow CD11b(+) cells did not show the same hyper-responsive phenotype as spleen and blood cells.Taken together, these results show significant promise for these mouse models to be used to study targeting innate immune pathways identified in human cells, thereby helping to understand the role the peripheral immune system plays in HD progression.

View Article: PubMed Central - PubMed

Affiliation: UCL Institute of Neurology, Dept. of Neurodegenerative Disease, London, UK.

No MeSH data available.


Related in: MedlinePlus

Myeloid cells from R6/2 mice demonstrate no functional changes in cell adhesion, migration and differentiation. (A) Bone marrow myeloid cells were isolated from 12-week old R6/2 and wild-type mice using anti-CD11b magnetic beads and were differentiated using M-CSF. Four days post seeding, adhered differentiated macrophages were detached and counted. (B) Peritoneal macrophages obtained via peritoneal lavage were seeded onto tissue culture plates. After 2 h the number of adhered cells was counted and no difference in adherence was observed when comparing wild-type and R6/2 cells. Using a transwell system, peritoneal macrophages from R6/2 mice did not show a difference in basal or MCP-1 induced migration after either 16 h or 90 min. Data shown as mean ± SEM. n = biological replicates representing individual mice. Unpaired two-tailed Student's t-tests.
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f0040: Myeloid cells from R6/2 mice demonstrate no functional changes in cell adhesion, migration and differentiation. (A) Bone marrow myeloid cells were isolated from 12-week old R6/2 and wild-type mice using anti-CD11b magnetic beads and were differentiated using M-CSF. Four days post seeding, adhered differentiated macrophages were detached and counted. (B) Peritoneal macrophages obtained via peritoneal lavage were seeded onto tissue culture plates. After 2 h the number of adhered cells was counted and no difference in adherence was observed when comparing wild-type and R6/2 cells. Using a transwell system, peritoneal macrophages from R6/2 mice did not show a difference in basal or MCP-1 induced migration after either 16 h or 90 min. Data shown as mean ± SEM. n = biological replicates representing individual mice. Unpaired two-tailed Student's t-tests.

Mentions: Counting the number of differentiated, adhered bone marrow-derived macrophages four days after seeding showed no difference in R6/2 compared with wild-type cells (Fig. 8A). In addition, adhesion of peritoneal macrophages appeared to be normal in R6/2 mice, as the same number of R6/2 and wild-type macrophages adhered to the tissue culture plastic 2 h after seeding (Fig. 8B).


Characterisation of immune cell function in fragment and full-length Huntington's disease mouse models.

Träger U, Andre R, Magnusson-Lind A, Miller JR, Connolly C, Weiss A, Grueninger S, Silajdžić E, Smith DL, Leavitt BR, Bates GP, Björkqvist M, Tabrizi SJ - Neurobiol. Dis. (2014)

Myeloid cells from R6/2 mice demonstrate no functional changes in cell adhesion, migration and differentiation. (A) Bone marrow myeloid cells were isolated from 12-week old R6/2 and wild-type mice using anti-CD11b magnetic beads and were differentiated using M-CSF. Four days post seeding, adhered differentiated macrophages were detached and counted. (B) Peritoneal macrophages obtained via peritoneal lavage were seeded onto tissue culture plates. After 2 h the number of adhered cells was counted and no difference in adherence was observed when comparing wild-type and R6/2 cells. Using a transwell system, peritoneal macrophages from R6/2 mice did not show a difference in basal or MCP-1 induced migration after either 16 h or 90 min. Data shown as mean ± SEM. n = biological replicates representing individual mice. Unpaired two-tailed Student's t-tests.
© Copyright Policy - CC BY-NC-ND
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC4262574&req=5

f0040: Myeloid cells from R6/2 mice demonstrate no functional changes in cell adhesion, migration and differentiation. (A) Bone marrow myeloid cells were isolated from 12-week old R6/2 and wild-type mice using anti-CD11b magnetic beads and were differentiated using M-CSF. Four days post seeding, adhered differentiated macrophages were detached and counted. (B) Peritoneal macrophages obtained via peritoneal lavage were seeded onto tissue culture plates. After 2 h the number of adhered cells was counted and no difference in adherence was observed when comparing wild-type and R6/2 cells. Using a transwell system, peritoneal macrophages from R6/2 mice did not show a difference in basal or MCP-1 induced migration after either 16 h or 90 min. Data shown as mean ± SEM. n = biological replicates representing individual mice. Unpaired two-tailed Student's t-tests.
Mentions: Counting the number of differentiated, adhered bone marrow-derived macrophages four days after seeding showed no difference in R6/2 compared with wild-type cells (Fig. 8A). In addition, adhesion of peritoneal macrophages appeared to be normal in R6/2 mice, as the same number of R6/2 and wild-type macrophages adhered to the tissue culture plastic 2 h after seeding (Fig. 8B).

Bottom Line: Inflammation is a growing area of research in neurodegeneration.However, bone marrow CD11b(+) cells did not show the same hyper-responsive phenotype as spleen and blood cells.Taken together, these results show significant promise for these mouse models to be used to study targeting innate immune pathways identified in human cells, thereby helping to understand the role the peripheral immune system plays in HD progression.

View Article: PubMed Central - PubMed

Affiliation: UCL Institute of Neurology, Dept. of Neurodegenerative Disease, London, UK.

No MeSH data available.


Related in: MedlinePlus