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Characterisation of immune cell function in fragment and full-length Huntington's disease mouse models.

Träger U, Andre R, Magnusson-Lind A, Miller JR, Connolly C, Weiss A, Grueninger S, Silajdžić E, Smith DL, Leavitt BR, Bates GP, Björkqvist M, Tabrizi SJ - Neurobiol. Dis. (2014)

Bottom Line: Inflammation is a growing area of research in neurodegeneration.However, bone marrow CD11b(+) cells did not show the same hyper-responsive phenotype as spleen and blood cells.Taken together, these results show significant promise for these mouse models to be used to study targeting innate immune pathways identified in human cells, thereby helping to understand the role the peripheral immune system plays in HD progression.

View Article: PubMed Central - PubMed

Affiliation: UCL Institute of Neurology, Dept. of Neurodegenerative Disease, London, UK.

No MeSH data available.


Related in: MedlinePlus

Splenic B cell, T cell and myeloid cell populations are unchanged in R6/2 mice. (A) Spleens from 12-week old R6/2 and wild-type mice were digested, homogenised and, after red blood cell lysis, stained with anti-CD11b PE, anti-CD3 APC and anti-CD19 FITC antibodies to look for myeloid, T and B cell subsets, respectively. The percentage of each cell population was determined via flow cytometry analysis using the gating as shown. (B) No differences were found comparing R6/2 and wild-type mice spleens using unpaired two-tailed Student's t-tests.
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f0025: Splenic B cell, T cell and myeloid cell populations are unchanged in R6/2 mice. (A) Spleens from 12-week old R6/2 and wild-type mice were digested, homogenised and, after red blood cell lysis, stained with anti-CD11b PE, anti-CD3 APC and anti-CD19 FITC antibodies to look for myeloid, T and B cell subsets, respectively. The percentage of each cell population was determined via flow cytometry analysis using the gating as shown. (B) No differences were found comparing R6/2 and wild-type mice spleens using unpaired two-tailed Student's t-tests.

Mentions: We also analysed the proportions of different immune cell subsets within the whole cell population of spleens from 12-week old R6/2 mice. Single cell suspensions prepared from digested spleens were stained with anti-CD3, anti-CD19 and anti-CD11b antibodies as markers for T cells, B cells and myeloid cells, respectively (Fig. 5A). No differences in the proportion of these immune cell populations were found in R6/2 as compared with wild-type spleens (Fig. 5B). Following this, spleen cells were stained with anti-CD3, anti-CD4 and anti-CD8 antibodies to investigate the abundance of CD4+ Thelper cells and CD8+ cytotoxic T cells in the spleens of R6/2 mice. However, no differences in T cell subsets were detected in R6/2 spleens when compared with those from wild-type mice (Fig. S4). We also examined myeloid cell subsets, in which spleen cells were stained with anti-F4/80 antibody as a macrophage marker and a combination of anti-CD11c, anti-B220 and anti-CD11b antibodies to distinguish between CD11c+ B220+ plasmacytoid DCs, CD11c+ B220− CD11b− lymphoid-derived DCs and CD11c+ B220− CD11b+ myeloid-derived DCs. Again, no differences in macrophage and DC subsets were detectable in R6/2 compared with wild-type spleens (Fig. S5).


Characterisation of immune cell function in fragment and full-length Huntington's disease mouse models.

Träger U, Andre R, Magnusson-Lind A, Miller JR, Connolly C, Weiss A, Grueninger S, Silajdžić E, Smith DL, Leavitt BR, Bates GP, Björkqvist M, Tabrizi SJ - Neurobiol. Dis. (2014)

Splenic B cell, T cell and myeloid cell populations are unchanged in R6/2 mice. (A) Spleens from 12-week old R6/2 and wild-type mice were digested, homogenised and, after red blood cell lysis, stained with anti-CD11b PE, anti-CD3 APC and anti-CD19 FITC antibodies to look for myeloid, T and B cell subsets, respectively. The percentage of each cell population was determined via flow cytometry analysis using the gating as shown. (B) No differences were found comparing R6/2 and wild-type mice spleens using unpaired two-tailed Student's t-tests.
© Copyright Policy - CC BY-NC-ND
Related In: Results  -  Collection

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Show All Figures
getmorefigures.php?uid=PMC4262574&req=5

f0025: Splenic B cell, T cell and myeloid cell populations are unchanged in R6/2 mice. (A) Spleens from 12-week old R6/2 and wild-type mice were digested, homogenised and, after red blood cell lysis, stained with anti-CD11b PE, anti-CD3 APC and anti-CD19 FITC antibodies to look for myeloid, T and B cell subsets, respectively. The percentage of each cell population was determined via flow cytometry analysis using the gating as shown. (B) No differences were found comparing R6/2 and wild-type mice spleens using unpaired two-tailed Student's t-tests.
Mentions: We also analysed the proportions of different immune cell subsets within the whole cell population of spleens from 12-week old R6/2 mice. Single cell suspensions prepared from digested spleens were stained with anti-CD3, anti-CD19 and anti-CD11b antibodies as markers for T cells, B cells and myeloid cells, respectively (Fig. 5A). No differences in the proportion of these immune cell populations were found in R6/2 as compared with wild-type spleens (Fig. 5B). Following this, spleen cells were stained with anti-CD3, anti-CD4 and anti-CD8 antibodies to investigate the abundance of CD4+ Thelper cells and CD8+ cytotoxic T cells in the spleens of R6/2 mice. However, no differences in T cell subsets were detected in R6/2 spleens when compared with those from wild-type mice (Fig. S4). We also examined myeloid cell subsets, in which spleen cells were stained with anti-F4/80 antibody as a macrophage marker and a combination of anti-CD11c, anti-B220 and anti-CD11b antibodies to distinguish between CD11c+ B220+ plasmacytoid DCs, CD11c+ B220− CD11b− lymphoid-derived DCs and CD11c+ B220− CD11b+ myeloid-derived DCs. Again, no differences in macrophage and DC subsets were detectable in R6/2 compared with wild-type spleens (Fig. S5).

Bottom Line: Inflammation is a growing area of research in neurodegeneration.However, bone marrow CD11b(+) cells did not show the same hyper-responsive phenotype as spleen and blood cells.Taken together, these results show significant promise for these mouse models to be used to study targeting innate immune pathways identified in human cells, thereby helping to understand the role the peripheral immune system plays in HD progression.

View Article: PubMed Central - PubMed

Affiliation: UCL Institute of Neurology, Dept. of Neurodegenerative Disease, London, UK.

No MeSH data available.


Related in: MedlinePlus