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miR‑542‑3p overexpression is associated with enhanced osteosarcoma cell proliferation and migration ability by targeting Van Gogh‑like 2.

Li H, Liu H, Pei J, Wang H, Lv H - Mol Med Rep (2014)

Bottom Line: It occurs predominantly in infants and adolescents, with an incidence of 4‑5 cases/100,000,000.Using a dual luciferase assay and western blot analysis, the present study confirmed that Van Gogh‑like 2, which is a non‑canonical Wnt pathway suppressor, was a target gene of miR‑542‑3p.Subsequently, the biological function of miR‑542‑3p in U2OS cells was examined, which revealed that overexpression of miR‑542‑3p can enhance the cell proliferation and migration ability of U2OS cells.

View Article: PubMed Central - PubMed

Affiliation: Department of Orthopedics, Yidu Central Hospital, Weifang Medical College, Weifang, Shandong 262500, P.R. China.

ABSTRACT
Osteosarcoma is the most common histological form of primary bone cancer, which arises from osteoid tissue. It occurs predominantly in infants and adolescents, with an incidence of 4‑5 cases/100,000,000. The 5-year survival rate of patients with osteosarcoma has significantly improved over time; however, there remains a significant proportion of patients that respond poorly to chemotherapy. An improved understanding of the pathology of osteosarcoma is required to provide more effective treatment strategies, identify biomarkers and develop novel chemotherapeutic agents. Disturbance in microRNA (miRNA) expression has been identified in osteosarcoma tissues and cell lines; however, the roles of miRNA during osteosarcoma pathogenesis remain to be elucidated. In the present study, the expression levels of eight selected miRNAs were investigated in osteosarcoma tissues and the results revealed that the expression levels of miR‑542‑3p and miR‑542‑5p were significantly upregulated and the expression of miR‑199‑3p was significantly downregulated. Using a dual luciferase assay and western blot analysis, the present study confirmed that Van Gogh‑like 2, which is a non‑canonical Wnt pathway suppressor, was a target gene of miR‑542‑3p. Subsequently, the biological function of miR‑542‑3p in U2OS cells was examined, which revealed that overexpression of miR‑542‑3p can enhance the cell proliferation and migration ability of U2OS cells. This indicated that miR‑542‑3p may act as an oncogene in osteosarcoma pathogenesis. The findings of the present study may provide assistance in understanding the development of osteosarcoma and aid in the development of strategies for the diagnosis and treatment of osteosarcoma.

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miR-542-3p enhances U2OS cell migration. U2OS cells were transfected with either the pre-miR control, miR-542-3p mimic, anti-miR control or miR-542-3p inhibitor. The cells were harvested 48 h after transfection, recounted to 0.5×106 cells/ml in every group and seeded into Transwells for a cell migration assay. Subsequently, the cells on the top of the membranes were removed and the cells on the bottom of the membranes were stained with hematoxylin and eosin. Cell migration was quantified by counting the number of cells passing through the membrane from five randomly selected fields in each sample 12 h after incubation (magnification, ×100). Representative photomicrographs of the cells passing through the membrane (magnification, ×100) are shown. Data are expressed as the mean of independent triplicate experiments. *P<0.05; **P<0.01. miR, microRNA.
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f4-mmr-11-02-0851: miR-542-3p enhances U2OS cell migration. U2OS cells were transfected with either the pre-miR control, miR-542-3p mimic, anti-miR control or miR-542-3p inhibitor. The cells were harvested 48 h after transfection, recounted to 0.5×106 cells/ml in every group and seeded into Transwells for a cell migration assay. Subsequently, the cells on the top of the membranes were removed and the cells on the bottom of the membranes were stained with hematoxylin and eosin. Cell migration was quantified by counting the number of cells passing through the membrane from five randomly selected fields in each sample 12 h after incubation (magnification, ×100). Representative photomicrographs of the cells passing through the membrane (magnification, ×100) are shown. Data are expressed as the mean of independent triplicate experiments. *P<0.05; **P<0.01. miR, microRNA.

Mentions: In order to investigate the role of miR-542-3p in the metastasis of osteosarcoma cells, the present study then analyzed the effects of miR-542-3p on the migratory behavior of osteosarcoma cells (Fig. 4). The results revealed that the migration capacity of the U2OS cells transfected with the miR-542-3p mimic were significantly higher compared with those transfected with the miR control (P<0.05). Conversely, the migration capacity was significantly suppressed in the U2OS cells transfected with the miR-542-3p inhibitor compared with the anti-miR control (P<0.01). These findings suggested that the level of miR-542-3p may be closely associated with the metastasis of osteosarcoma cells.


miR‑542‑3p overexpression is associated with enhanced osteosarcoma cell proliferation and migration ability by targeting Van Gogh‑like 2.

Li H, Liu H, Pei J, Wang H, Lv H - Mol Med Rep (2014)

miR-542-3p enhances U2OS cell migration. U2OS cells were transfected with either the pre-miR control, miR-542-3p mimic, anti-miR control or miR-542-3p inhibitor. The cells were harvested 48 h after transfection, recounted to 0.5×106 cells/ml in every group and seeded into Transwells for a cell migration assay. Subsequently, the cells on the top of the membranes were removed and the cells on the bottom of the membranes were stained with hematoxylin and eosin. Cell migration was quantified by counting the number of cells passing through the membrane from five randomly selected fields in each sample 12 h after incubation (magnification, ×100). Representative photomicrographs of the cells passing through the membrane (magnification, ×100) are shown. Data are expressed as the mean of independent triplicate experiments. *P<0.05; **P<0.01. miR, microRNA.
© Copyright Policy - open-access
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC4262515&req=5

f4-mmr-11-02-0851: miR-542-3p enhances U2OS cell migration. U2OS cells were transfected with either the pre-miR control, miR-542-3p mimic, anti-miR control or miR-542-3p inhibitor. The cells were harvested 48 h after transfection, recounted to 0.5×106 cells/ml in every group and seeded into Transwells for a cell migration assay. Subsequently, the cells on the top of the membranes were removed and the cells on the bottom of the membranes were stained with hematoxylin and eosin. Cell migration was quantified by counting the number of cells passing through the membrane from five randomly selected fields in each sample 12 h after incubation (magnification, ×100). Representative photomicrographs of the cells passing through the membrane (magnification, ×100) are shown. Data are expressed as the mean of independent triplicate experiments. *P<0.05; **P<0.01. miR, microRNA.
Mentions: In order to investigate the role of miR-542-3p in the metastasis of osteosarcoma cells, the present study then analyzed the effects of miR-542-3p on the migratory behavior of osteosarcoma cells (Fig. 4). The results revealed that the migration capacity of the U2OS cells transfected with the miR-542-3p mimic were significantly higher compared with those transfected with the miR control (P<0.05). Conversely, the migration capacity was significantly suppressed in the U2OS cells transfected with the miR-542-3p inhibitor compared with the anti-miR control (P<0.01). These findings suggested that the level of miR-542-3p may be closely associated with the metastasis of osteosarcoma cells.

Bottom Line: It occurs predominantly in infants and adolescents, with an incidence of 4‑5 cases/100,000,000.Using a dual luciferase assay and western blot analysis, the present study confirmed that Van Gogh‑like 2, which is a non‑canonical Wnt pathway suppressor, was a target gene of miR‑542‑3p.Subsequently, the biological function of miR‑542‑3p in U2OS cells was examined, which revealed that overexpression of miR‑542‑3p can enhance the cell proliferation and migration ability of U2OS cells.

View Article: PubMed Central - PubMed

Affiliation: Department of Orthopedics, Yidu Central Hospital, Weifang Medical College, Weifang, Shandong 262500, P.R. China.

ABSTRACT
Osteosarcoma is the most common histological form of primary bone cancer, which arises from osteoid tissue. It occurs predominantly in infants and adolescents, with an incidence of 4‑5 cases/100,000,000. The 5-year survival rate of patients with osteosarcoma has significantly improved over time; however, there remains a significant proportion of patients that respond poorly to chemotherapy. An improved understanding of the pathology of osteosarcoma is required to provide more effective treatment strategies, identify biomarkers and develop novel chemotherapeutic agents. Disturbance in microRNA (miRNA) expression has been identified in osteosarcoma tissues and cell lines; however, the roles of miRNA during osteosarcoma pathogenesis remain to be elucidated. In the present study, the expression levels of eight selected miRNAs were investigated in osteosarcoma tissues and the results revealed that the expression levels of miR‑542‑3p and miR‑542‑5p were significantly upregulated and the expression of miR‑199‑3p was significantly downregulated. Using a dual luciferase assay and western blot analysis, the present study confirmed that Van Gogh‑like 2, which is a non‑canonical Wnt pathway suppressor, was a target gene of miR‑542‑3p. Subsequently, the biological function of miR‑542‑3p in U2OS cells was examined, which revealed that overexpression of miR‑542‑3p can enhance the cell proliferation and migration ability of U2OS cells. This indicated that miR‑542‑3p may act as an oncogene in osteosarcoma pathogenesis. The findings of the present study may provide assistance in understanding the development of osteosarcoma and aid in the development of strategies for the diagnosis and treatment of osteosarcoma.

Show MeSH
Related in: MedlinePlus