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miR‑542‑3p overexpression is associated with enhanced osteosarcoma cell proliferation and migration ability by targeting Van Gogh‑like 2.

Li H, Liu H, Pei J, Wang H, Lv H - Mol Med Rep (2014)

Bottom Line: It occurs predominantly in infants and adolescents, with an incidence of 4‑5 cases/100,000,000.Using a dual luciferase assay and western blot analysis, the present study confirmed that Van Gogh‑like 2, which is a non‑canonical Wnt pathway suppressor, was a target gene of miR‑542‑3p.Subsequently, the biological function of miR‑542‑3p in U2OS cells was examined, which revealed that overexpression of miR‑542‑3p can enhance the cell proliferation and migration ability of U2OS cells.

View Article: PubMed Central - PubMed

Affiliation: Department of Orthopedics, Yidu Central Hospital, Weifang Medical College, Weifang, Shandong 262500, P.R. China.

ABSTRACT
Osteosarcoma is the most common histological form of primary bone cancer, which arises from osteoid tissue. It occurs predominantly in infants and adolescents, with an incidence of 4‑5 cases/100,000,000. The 5-year survival rate of patients with osteosarcoma has significantly improved over time; however, there remains a significant proportion of patients that respond poorly to chemotherapy. An improved understanding of the pathology of osteosarcoma is required to provide more effective treatment strategies, identify biomarkers and develop novel chemotherapeutic agents. Disturbance in microRNA (miRNA) expression has been identified in osteosarcoma tissues and cell lines; however, the roles of miRNA during osteosarcoma pathogenesis remain to be elucidated. In the present study, the expression levels of eight selected miRNAs were investigated in osteosarcoma tissues and the results revealed that the expression levels of miR‑542‑3p and miR‑542‑5p were significantly upregulated and the expression of miR‑199‑3p was significantly downregulated. Using a dual luciferase assay and western blot analysis, the present study confirmed that Van Gogh‑like 2, which is a non‑canonical Wnt pathway suppressor, was a target gene of miR‑542‑3p. Subsequently, the biological function of miR‑542‑3p in U2OS cells was examined, which revealed that overexpression of miR‑542‑3p can enhance the cell proliferation and migration ability of U2OS cells. This indicated that miR‑542‑3p may act as an oncogene in osteosarcoma pathogenesis. The findings of the present study may provide assistance in understanding the development of osteosarcoma and aid in the development of strategies for the diagnosis and treatment of osteosarcoma.

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U2OS cells are enhanced by miR-542-3p. (A) Reverse transcription-quantitative polymerase chain reaction was used to detect the expression of miR-542-3p in the transfected U2OS cells. (B) An MTT assay was used to detect cell proliferation ability. miR, microRNA; OD, optical density.
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f3-mmr-11-02-0851: U2OS cells are enhanced by miR-542-3p. (A) Reverse transcription-quantitative polymerase chain reaction was used to detect the expression of miR-542-3p in the transfected U2OS cells. (B) An MTT assay was used to detect cell proliferation ability. miR, microRNA; OD, optical density.

Mentions: The U2OS cells were transfected with either the miR-542-3p mimic, miR control, miR-542-3p inhibitor or anti-miR control. The results demonstrated that the expression level of miR-542-3p in the U20S cells was increased in the cells transfected with the miR-542-3p mimic compared with the pre-miR control (P<0.01) and was decreased in the cells transfected with the miR-542-3p inhibitor compared with the anti-miR control (P<0.05) as shown in Fig. 3A.


miR‑542‑3p overexpression is associated with enhanced osteosarcoma cell proliferation and migration ability by targeting Van Gogh‑like 2.

Li H, Liu H, Pei J, Wang H, Lv H - Mol Med Rep (2014)

U2OS cells are enhanced by miR-542-3p. (A) Reverse transcription-quantitative polymerase chain reaction was used to detect the expression of miR-542-3p in the transfected U2OS cells. (B) An MTT assay was used to detect cell proliferation ability. miR, microRNA; OD, optical density.
© Copyright Policy - open-access
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC4262515&req=5

f3-mmr-11-02-0851: U2OS cells are enhanced by miR-542-3p. (A) Reverse transcription-quantitative polymerase chain reaction was used to detect the expression of miR-542-3p in the transfected U2OS cells. (B) An MTT assay was used to detect cell proliferation ability. miR, microRNA; OD, optical density.
Mentions: The U2OS cells were transfected with either the miR-542-3p mimic, miR control, miR-542-3p inhibitor or anti-miR control. The results demonstrated that the expression level of miR-542-3p in the U20S cells was increased in the cells transfected with the miR-542-3p mimic compared with the pre-miR control (P<0.01) and was decreased in the cells transfected with the miR-542-3p inhibitor compared with the anti-miR control (P<0.05) as shown in Fig. 3A.

Bottom Line: It occurs predominantly in infants and adolescents, with an incidence of 4‑5 cases/100,000,000.Using a dual luciferase assay and western blot analysis, the present study confirmed that Van Gogh‑like 2, which is a non‑canonical Wnt pathway suppressor, was a target gene of miR‑542‑3p.Subsequently, the biological function of miR‑542‑3p in U2OS cells was examined, which revealed that overexpression of miR‑542‑3p can enhance the cell proliferation and migration ability of U2OS cells.

View Article: PubMed Central - PubMed

Affiliation: Department of Orthopedics, Yidu Central Hospital, Weifang Medical College, Weifang, Shandong 262500, P.R. China.

ABSTRACT
Osteosarcoma is the most common histological form of primary bone cancer, which arises from osteoid tissue. It occurs predominantly in infants and adolescents, with an incidence of 4‑5 cases/100,000,000. The 5-year survival rate of patients with osteosarcoma has significantly improved over time; however, there remains a significant proportion of patients that respond poorly to chemotherapy. An improved understanding of the pathology of osteosarcoma is required to provide more effective treatment strategies, identify biomarkers and develop novel chemotherapeutic agents. Disturbance in microRNA (miRNA) expression has been identified in osteosarcoma tissues and cell lines; however, the roles of miRNA during osteosarcoma pathogenesis remain to be elucidated. In the present study, the expression levels of eight selected miRNAs were investigated in osteosarcoma tissues and the results revealed that the expression levels of miR‑542‑3p and miR‑542‑5p were significantly upregulated and the expression of miR‑199‑3p was significantly downregulated. Using a dual luciferase assay and western blot analysis, the present study confirmed that Van Gogh‑like 2, which is a non‑canonical Wnt pathway suppressor, was a target gene of miR‑542‑3p. Subsequently, the biological function of miR‑542‑3p in U2OS cells was examined, which revealed that overexpression of miR‑542‑3p can enhance the cell proliferation and migration ability of U2OS cells. This indicated that miR‑542‑3p may act as an oncogene in osteosarcoma pathogenesis. The findings of the present study may provide assistance in understanding the development of osteosarcoma and aid in the development of strategies for the diagnosis and treatment of osteosarcoma.

Show MeSH
Related in: MedlinePlus