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miR‑542‑3p overexpression is associated with enhanced osteosarcoma cell proliferation and migration ability by targeting Van Gogh‑like 2.

Li H, Liu H, Pei J, Wang H, Lv H - Mol Med Rep (2014)

Bottom Line: It occurs predominantly in infants and adolescents, with an incidence of 4‑5 cases/100,000,000.Using a dual luciferase assay and western blot analysis, the present study confirmed that Van Gogh‑like 2, which is a non‑canonical Wnt pathway suppressor, was a target gene of miR‑542‑3p.Subsequently, the biological function of miR‑542‑3p in U2OS cells was examined, which revealed that overexpression of miR‑542‑3p can enhance the cell proliferation and migration ability of U2OS cells.

View Article: PubMed Central - PubMed

Affiliation: Department of Orthopedics, Yidu Central Hospital, Weifang Medical College, Weifang, Shandong 262500, P.R. China.

ABSTRACT
Osteosarcoma is the most common histological form of primary bone cancer, which arises from osteoid tissue. It occurs predominantly in infants and adolescents, with an incidence of 4‑5 cases/100,000,000. The 5-year survival rate of patients with osteosarcoma has significantly improved over time; however, there remains a significant proportion of patients that respond poorly to chemotherapy. An improved understanding of the pathology of osteosarcoma is required to provide more effective treatment strategies, identify biomarkers and develop novel chemotherapeutic agents. Disturbance in microRNA (miRNA) expression has been identified in osteosarcoma tissues and cell lines; however, the roles of miRNA during osteosarcoma pathogenesis remain to be elucidated. In the present study, the expression levels of eight selected miRNAs were investigated in osteosarcoma tissues and the results revealed that the expression levels of miR‑542‑3p and miR‑542‑5p were significantly upregulated and the expression of miR‑199‑3p was significantly downregulated. Using a dual luciferase assay and western blot analysis, the present study confirmed that Van Gogh‑like 2, which is a non‑canonical Wnt pathway suppressor, was a target gene of miR‑542‑3p. Subsequently, the biological function of miR‑542‑3p in U2OS cells was examined, which revealed that overexpression of miR‑542‑3p can enhance the cell proliferation and migration ability of U2OS cells. This indicated that miR‑542‑3p may act as an oncogene in osteosarcoma pathogenesis. The findings of the present study may provide assistance in understanding the development of osteosarcoma and aid in the development of strategies for the diagnosis and treatment of osteosarcoma.

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Disturbed miRNA expression in osteosarcoma tissue samples. The expression level of eight candidate miRNAs was detected in individual samples using TaqMan miRNA reverse transcription-quantitative polymerase chain reaction. Statistical analyses were performed to analyze the overall trend of each miRNA in all osteosarcoma tissue samples. U6 was used as an internal reference among the different samples and to normalize for experimental error. *P<0.05. miRNA and miR, microRNA; NC, normal control; OB, osteosarcoma.
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f1-mmr-11-02-0851: Disturbed miRNA expression in osteosarcoma tissue samples. The expression level of eight candidate miRNAs was detected in individual samples using TaqMan miRNA reverse transcription-quantitative polymerase chain reaction. Statistical analyses were performed to analyze the overall trend of each miRNA in all osteosarcoma tissue samples. U6 was used as an internal reference among the different samples and to normalize for experimental error. *P<0.05. miRNA and miR, microRNA; NC, normal control; OB, osteosarcoma.

Mentions: Several studies have reported that the miRNA expression profile is altered significantly in the progression of osteosarcoma (9,10), however, further clarification is required. In the present study, the expression profiles of eight miRNAs, which were identified by another study as differentially expressed in osteosarcoma tissues or osteosarcoma cell lines (11), were determined by RT-qPCR. As shown in Fig. 1, miR-542-3p and miR-542-5p were significantly upregulated and miR-199-3p was significantly downregulated in the osteosarcoma tissues.


miR‑542‑3p overexpression is associated with enhanced osteosarcoma cell proliferation and migration ability by targeting Van Gogh‑like 2.

Li H, Liu H, Pei J, Wang H, Lv H - Mol Med Rep (2014)

Disturbed miRNA expression in osteosarcoma tissue samples. The expression level of eight candidate miRNAs was detected in individual samples using TaqMan miRNA reverse transcription-quantitative polymerase chain reaction. Statistical analyses were performed to analyze the overall trend of each miRNA in all osteosarcoma tissue samples. U6 was used as an internal reference among the different samples and to normalize for experimental error. *P<0.05. miRNA and miR, microRNA; NC, normal control; OB, osteosarcoma.
© Copyright Policy - open-access
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC4262515&req=5

f1-mmr-11-02-0851: Disturbed miRNA expression in osteosarcoma tissue samples. The expression level of eight candidate miRNAs was detected in individual samples using TaqMan miRNA reverse transcription-quantitative polymerase chain reaction. Statistical analyses were performed to analyze the overall trend of each miRNA in all osteosarcoma tissue samples. U6 was used as an internal reference among the different samples and to normalize for experimental error. *P<0.05. miRNA and miR, microRNA; NC, normal control; OB, osteosarcoma.
Mentions: Several studies have reported that the miRNA expression profile is altered significantly in the progression of osteosarcoma (9,10), however, further clarification is required. In the present study, the expression profiles of eight miRNAs, which were identified by another study as differentially expressed in osteosarcoma tissues or osteosarcoma cell lines (11), were determined by RT-qPCR. As shown in Fig. 1, miR-542-3p and miR-542-5p were significantly upregulated and miR-199-3p was significantly downregulated in the osteosarcoma tissues.

Bottom Line: It occurs predominantly in infants and adolescents, with an incidence of 4‑5 cases/100,000,000.Using a dual luciferase assay and western blot analysis, the present study confirmed that Van Gogh‑like 2, which is a non‑canonical Wnt pathway suppressor, was a target gene of miR‑542‑3p.Subsequently, the biological function of miR‑542‑3p in U2OS cells was examined, which revealed that overexpression of miR‑542‑3p can enhance the cell proliferation and migration ability of U2OS cells.

View Article: PubMed Central - PubMed

Affiliation: Department of Orthopedics, Yidu Central Hospital, Weifang Medical College, Weifang, Shandong 262500, P.R. China.

ABSTRACT
Osteosarcoma is the most common histological form of primary bone cancer, which arises from osteoid tissue. It occurs predominantly in infants and adolescents, with an incidence of 4‑5 cases/100,000,000. The 5-year survival rate of patients with osteosarcoma has significantly improved over time; however, there remains a significant proportion of patients that respond poorly to chemotherapy. An improved understanding of the pathology of osteosarcoma is required to provide more effective treatment strategies, identify biomarkers and develop novel chemotherapeutic agents. Disturbance in microRNA (miRNA) expression has been identified in osteosarcoma tissues and cell lines; however, the roles of miRNA during osteosarcoma pathogenesis remain to be elucidated. In the present study, the expression levels of eight selected miRNAs were investigated in osteosarcoma tissues and the results revealed that the expression levels of miR‑542‑3p and miR‑542‑5p were significantly upregulated and the expression of miR‑199‑3p was significantly downregulated. Using a dual luciferase assay and western blot analysis, the present study confirmed that Van Gogh‑like 2, which is a non‑canonical Wnt pathway suppressor, was a target gene of miR‑542‑3p. Subsequently, the biological function of miR‑542‑3p in U2OS cells was examined, which revealed that overexpression of miR‑542‑3p can enhance the cell proliferation and migration ability of U2OS cells. This indicated that miR‑542‑3p may act as an oncogene in osteosarcoma pathogenesis. The findings of the present study may provide assistance in understanding the development of osteosarcoma and aid in the development of strategies for the diagnosis and treatment of osteosarcoma.

Show MeSH
Related in: MedlinePlus