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Triptolide inhibits cell proliferation and tumorigenicity of human neuroblastoma cells.

Yan X, Ke XX, Zhao H, Huang M, Hu R, Cui H - Mol Med Rep (2014)

Bottom Line: Reverse transcription‑quantitative polymerase chain reaction was conducted to detect the expression levels of the apoptosis‑associated proteins, caspase‑3 and caspase‑9.The results demonstrated that exposure of BE(2)‑C human neuroblastoma cells to triptolide resulted in a reduction in cell growth and proliferation, and the induction of cell death and apoptosis, together with cell cycle arrest in the S phase.The xenograft experiment showed that triptolide significantly reduced tumor growth and development in vivo.

View Article: PubMed Central - PubMed

Affiliation: State Key Laboratory of Silkworm Genome Biology, Southwest University, Chongqing 400716, P.R. China.

ABSTRACT
Triptolide is a diterpene triepoxide, extracted from the Chinese herb Tripterygium wilfordii Hook F, which has been shown to have antitumor activity in a number of cancers. Neuroblastoma is an aggressive extracranial pediatric solid tumor, with significant chemotherapeutic resistance. In this study, triptolide was hypothesized to be a potential therapeutic agent for neuroblastoma. The effects of triptolide on neuroblastoma cell growth and tumor development were investigated. Cell growth and proliferation were evaluated using a cell counting kit‑8 assay and a 5-bromo-2-deoxyuridine staining assay. Cell cycle and apoptosis were detected by flow cytometry. Reverse transcription‑quantitative polymerase chain reaction was conducted to detect the expression levels of the apoptosis‑associated proteins, caspase‑3 and caspase‑9. The tumorigenicity of neuroblastoma cells was assessed by a soft agar clonogenic assay and an in vivo tumorigenic assay. The results demonstrated that exposure of BE(2)‑C human neuroblastoma cells to triptolide resulted in a reduction in cell growth and proliferation, and the induction of cell death and apoptosis, together with cell cycle arrest in the S phase. A soft agar assay indicated that triptolide inhibited the colony‑forming ability of BE(2)‑C neuroblastoma cells. The xenograft experiment showed that triptolide significantly reduced tumor growth and development in vivo. The data suggested that this Chinese herb may be a potential novel chemotherapeutic agent for neuroblastoma.

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Triptolide suppressed BE (2)-C cells colony-forming capability. (A) Soft agar clonogenic assay of BE(2)-C cells was performed after treatment with triptolide. After 14 days of culture, images of colonies (larger than 1.0 mm or containing more than 150 cells) were captured. Scale bars, 50 μm. (B) Analysis of colony formation numbers from panel A was performed. Cells were counted from at least five randomly selected fields. Data are presented as the mean ± standard deviation. *P<0.05 and **P<0.01, compared with control. DMSO, dimethyl sulfoxide.
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f5-mmr-11-02-0791: Triptolide suppressed BE (2)-C cells colony-forming capability. (A) Soft agar clonogenic assay of BE(2)-C cells was performed after treatment with triptolide. After 14 days of culture, images of colonies (larger than 1.0 mm or containing more than 150 cells) were captured. Scale bars, 50 μm. (B) Analysis of colony formation numbers from panel A was performed. Cells were counted from at least five randomly selected fields. Data are presented as the mean ± standard deviation. *P<0.05 and **P<0.01, compared with control. DMSO, dimethyl sulfoxide.

Mentions: The role of triptolide in neuroblastoma tumorigenesis was examined. BE(2)-C cells treated with 25 nM triptolide gave rise to smaller and and sparser colonies in soft agar, compared with cells treated with DMSO (Fig. 5A and B). The xenograft study in NOD/SCID mice showed that the volume and weight of xenograft tumors in the triptolide treatment group were lower than those in the DMSO group (Fig. 6). These data indicate that triptolide may inhibit neuroblastoma cell self-renewal and tumorigenesis. In addition, there was no significant difference in mouse body weight after triptolide treatment (Fig. 6D), which suggests that the administered dose of triptolide may have minimal toxic side effects.


Triptolide inhibits cell proliferation and tumorigenicity of human neuroblastoma cells.

Yan X, Ke XX, Zhao H, Huang M, Hu R, Cui H - Mol Med Rep (2014)

Triptolide suppressed BE (2)-C cells colony-forming capability. (A) Soft agar clonogenic assay of BE(2)-C cells was performed after treatment with triptolide. After 14 days of culture, images of colonies (larger than 1.0 mm or containing more than 150 cells) were captured. Scale bars, 50 μm. (B) Analysis of colony formation numbers from panel A was performed. Cells were counted from at least five randomly selected fields. Data are presented as the mean ± standard deviation. *P<0.05 and **P<0.01, compared with control. DMSO, dimethyl sulfoxide.
© Copyright Policy - open-access
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC4262511&req=5

f5-mmr-11-02-0791: Triptolide suppressed BE (2)-C cells colony-forming capability. (A) Soft agar clonogenic assay of BE(2)-C cells was performed after treatment with triptolide. After 14 days of culture, images of colonies (larger than 1.0 mm or containing more than 150 cells) were captured. Scale bars, 50 μm. (B) Analysis of colony formation numbers from panel A was performed. Cells were counted from at least five randomly selected fields. Data are presented as the mean ± standard deviation. *P<0.05 and **P<0.01, compared with control. DMSO, dimethyl sulfoxide.
Mentions: The role of triptolide in neuroblastoma tumorigenesis was examined. BE(2)-C cells treated with 25 nM triptolide gave rise to smaller and and sparser colonies in soft agar, compared with cells treated with DMSO (Fig. 5A and B). The xenograft study in NOD/SCID mice showed that the volume and weight of xenograft tumors in the triptolide treatment group were lower than those in the DMSO group (Fig. 6). These data indicate that triptolide may inhibit neuroblastoma cell self-renewal and tumorigenesis. In addition, there was no significant difference in mouse body weight after triptolide treatment (Fig. 6D), which suggests that the administered dose of triptolide may have minimal toxic side effects.

Bottom Line: Reverse transcription‑quantitative polymerase chain reaction was conducted to detect the expression levels of the apoptosis‑associated proteins, caspase‑3 and caspase‑9.The results demonstrated that exposure of BE(2)‑C human neuroblastoma cells to triptolide resulted in a reduction in cell growth and proliferation, and the induction of cell death and apoptosis, together with cell cycle arrest in the S phase.The xenograft experiment showed that triptolide significantly reduced tumor growth and development in vivo.

View Article: PubMed Central - PubMed

Affiliation: State Key Laboratory of Silkworm Genome Biology, Southwest University, Chongqing 400716, P.R. China.

ABSTRACT
Triptolide is a diterpene triepoxide, extracted from the Chinese herb Tripterygium wilfordii Hook F, which has been shown to have antitumor activity in a number of cancers. Neuroblastoma is an aggressive extracranial pediatric solid tumor, with significant chemotherapeutic resistance. In this study, triptolide was hypothesized to be a potential therapeutic agent for neuroblastoma. The effects of triptolide on neuroblastoma cell growth and tumor development were investigated. Cell growth and proliferation were evaluated using a cell counting kit‑8 assay and a 5-bromo-2-deoxyuridine staining assay. Cell cycle and apoptosis were detected by flow cytometry. Reverse transcription‑quantitative polymerase chain reaction was conducted to detect the expression levels of the apoptosis‑associated proteins, caspase‑3 and caspase‑9. The tumorigenicity of neuroblastoma cells was assessed by a soft agar clonogenic assay and an in vivo tumorigenic assay. The results demonstrated that exposure of BE(2)‑C human neuroblastoma cells to triptolide resulted in a reduction in cell growth and proliferation, and the induction of cell death and apoptosis, together with cell cycle arrest in the S phase. A soft agar assay indicated that triptolide inhibited the colony‑forming ability of BE(2)‑C neuroblastoma cells. The xenograft experiment showed that triptolide significantly reduced tumor growth and development in vivo. The data suggested that this Chinese herb may be a potential novel chemotherapeutic agent for neuroblastoma.

Show MeSH
Related in: MedlinePlus