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Triptolide inhibits cell proliferation and tumorigenicity of human neuroblastoma cells.

Yan X, Ke XX, Zhao H, Huang M, Hu R, Cui H - Mol Med Rep (2014)

Bottom Line: Reverse transcription‑quantitative polymerase chain reaction was conducted to detect the expression levels of the apoptosis‑associated proteins, caspase‑3 and caspase‑9.The results demonstrated that exposure of BE(2)‑C human neuroblastoma cells to triptolide resulted in a reduction in cell growth and proliferation, and the induction of cell death and apoptosis, together with cell cycle arrest in the S phase.The xenograft experiment showed that triptolide significantly reduced tumor growth and development in vivo.

View Article: PubMed Central - PubMed

Affiliation: State Key Laboratory of Silkworm Genome Biology, Southwest University, Chongqing 400716, P.R. China.

ABSTRACT
Triptolide is a diterpene triepoxide, extracted from the Chinese herb Tripterygium wilfordii Hook F, which has been shown to have antitumor activity in a number of cancers. Neuroblastoma is an aggressive extracranial pediatric solid tumor, with significant chemotherapeutic resistance. In this study, triptolide was hypothesized to be a potential therapeutic agent for neuroblastoma. The effects of triptolide on neuroblastoma cell growth and tumor development were investigated. Cell growth and proliferation were evaluated using a cell counting kit‑8 assay and a 5-bromo-2-deoxyuridine staining assay. Cell cycle and apoptosis were detected by flow cytometry. Reverse transcription‑quantitative polymerase chain reaction was conducted to detect the expression levels of the apoptosis‑associated proteins, caspase‑3 and caspase‑9. The tumorigenicity of neuroblastoma cells was assessed by a soft agar clonogenic assay and an in vivo tumorigenic assay. The results demonstrated that exposure of BE(2)‑C human neuroblastoma cells to triptolide resulted in a reduction in cell growth and proliferation, and the induction of cell death and apoptosis, together with cell cycle arrest in the S phase. A soft agar assay indicated that triptolide inhibited the colony‑forming ability of BE(2)‑C neuroblastoma cells. The xenograft experiment showed that triptolide significantly reduced tumor growth and development in vivo. The data suggested that this Chinese herb may be a potential novel chemotherapeutic agent for neuroblastoma.

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Triptolide induced neuroblastoma cell cycle arrest in the S phase. (A) BE(2)-C cells were either treated with DMSO or 25 nM triptolide for 24 h. Cells were harvested, fixed with ethanol and stained with propidium iodide. DNA content was determined by flow cytometry. (B) Analysis of cell cycle phase percentage in BE(2)-C cells from panel A. Each column represents the average obtained from three independent experiments. Data are presented as the mean ± standard deviation. *P<0.05 and **P<0.01, compared with control. DMSO, dimethyl sulfoxide.
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f3-mmr-11-02-0791: Triptolide induced neuroblastoma cell cycle arrest in the S phase. (A) BE(2)-C cells were either treated with DMSO or 25 nM triptolide for 24 h. Cells were harvested, fixed with ethanol and stained with propidium iodide. DNA content was determined by flow cytometry. (B) Analysis of cell cycle phase percentage in BE(2)-C cells from panel A. Each column represents the average obtained from three independent experiments. Data are presented as the mean ± standard deviation. *P<0.05 and **P<0.01, compared with control. DMSO, dimethyl sulfoxide.

Mentions: The effect of triptolide on cell cycle was investigated. It was found that the percentage of cells in S phase increased from 36.06 to 58.16% (Fig. 3A and B). This result suggests that triptolide induces cell cycle arrest in the S phase, which may contribute to inhibition of cell proliferation.


Triptolide inhibits cell proliferation and tumorigenicity of human neuroblastoma cells.

Yan X, Ke XX, Zhao H, Huang M, Hu R, Cui H - Mol Med Rep (2014)

Triptolide induced neuroblastoma cell cycle arrest in the S phase. (A) BE(2)-C cells were either treated with DMSO or 25 nM triptolide for 24 h. Cells were harvested, fixed with ethanol and stained with propidium iodide. DNA content was determined by flow cytometry. (B) Analysis of cell cycle phase percentage in BE(2)-C cells from panel A. Each column represents the average obtained from three independent experiments. Data are presented as the mean ± standard deviation. *P<0.05 and **P<0.01, compared with control. DMSO, dimethyl sulfoxide.
© Copyright Policy - open-access
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC4262511&req=5

f3-mmr-11-02-0791: Triptolide induced neuroblastoma cell cycle arrest in the S phase. (A) BE(2)-C cells were either treated with DMSO or 25 nM triptolide for 24 h. Cells were harvested, fixed with ethanol and stained with propidium iodide. DNA content was determined by flow cytometry. (B) Analysis of cell cycle phase percentage in BE(2)-C cells from panel A. Each column represents the average obtained from three independent experiments. Data are presented as the mean ± standard deviation. *P<0.05 and **P<0.01, compared with control. DMSO, dimethyl sulfoxide.
Mentions: The effect of triptolide on cell cycle was investigated. It was found that the percentage of cells in S phase increased from 36.06 to 58.16% (Fig. 3A and B). This result suggests that triptolide induces cell cycle arrest in the S phase, which may contribute to inhibition of cell proliferation.

Bottom Line: Reverse transcription‑quantitative polymerase chain reaction was conducted to detect the expression levels of the apoptosis‑associated proteins, caspase‑3 and caspase‑9.The results demonstrated that exposure of BE(2)‑C human neuroblastoma cells to triptolide resulted in a reduction in cell growth and proliferation, and the induction of cell death and apoptosis, together with cell cycle arrest in the S phase.The xenograft experiment showed that triptolide significantly reduced tumor growth and development in vivo.

View Article: PubMed Central - PubMed

Affiliation: State Key Laboratory of Silkworm Genome Biology, Southwest University, Chongqing 400716, P.R. China.

ABSTRACT
Triptolide is a diterpene triepoxide, extracted from the Chinese herb Tripterygium wilfordii Hook F, which has been shown to have antitumor activity in a number of cancers. Neuroblastoma is an aggressive extracranial pediatric solid tumor, with significant chemotherapeutic resistance. In this study, triptolide was hypothesized to be a potential therapeutic agent for neuroblastoma. The effects of triptolide on neuroblastoma cell growth and tumor development were investigated. Cell growth and proliferation were evaluated using a cell counting kit‑8 assay and a 5-bromo-2-deoxyuridine staining assay. Cell cycle and apoptosis were detected by flow cytometry. Reverse transcription‑quantitative polymerase chain reaction was conducted to detect the expression levels of the apoptosis‑associated proteins, caspase‑3 and caspase‑9. The tumorigenicity of neuroblastoma cells was assessed by a soft agar clonogenic assay and an in vivo tumorigenic assay. The results demonstrated that exposure of BE(2)‑C human neuroblastoma cells to triptolide resulted in a reduction in cell growth and proliferation, and the induction of cell death and apoptosis, together with cell cycle arrest in the S phase. A soft agar assay indicated that triptolide inhibited the colony‑forming ability of BE(2)‑C neuroblastoma cells. The xenograft experiment showed that triptolide significantly reduced tumor growth and development in vivo. The data suggested that this Chinese herb may be a potential novel chemotherapeutic agent for neuroblastoma.

Show MeSH
Related in: MedlinePlus