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Near‑infrared fluorescence imaging of prostate cancer using heptamethine carbocyanine dyes.

Yuan J, Yi X, Yan F, Wang F, Qin W, Wu G, Yang X, Shao C, Chung LW - Mol Med Rep (2014)

Bottom Line: A previous study defined two organic polymethine cyanine dyes as ideal NIRF probes, IR‑783 and its derivative MHI‑148, which have excellent optical characteristics, superior biocompatibility and cancer targeting abilities.Different organic anion transporting peptide (OATP) inhibitors were utilized to explore the potential role of the OATP subtype, including the nonspecific OATP inhibitor bromosulfophthalein, the OATP1 inhibitor 17β‑estradiol, the selective OATP1B1 inhibitor rifampicin and the selective OATP1B3 inhibitor cholecystokinin octapeptide.The results of the present study demonstrated that NIRF dye‑mediated imaging is a feasible and practicable method for prostate cancer detection, although further investigative studies are required before clinical translation.

View Article: PubMed Central - PubMed

Affiliation: Department of Urology, Xijing Hospital, Fourth Military Medical University, Xi'an, Shaanxi 710032, P.R. China.

ABSTRACT
Near‑infrared fluorescence (NIRF) imaging is an attractive novel modality for the detection of cancer. A previous study defined two organic polymethine cyanine dyes as ideal NIRF probes, IR‑783 and its derivative MHI‑148, which have excellent optical characteristics, superior biocompatibility and cancer targeting abilities. To investigate the feasibility of NIRF dye‑mediated prostate cancer imaging, dye uptake and subcellular co‑localization were investigated in PC‑3, DU‑145 and LNCaP human prostate cancer cells and RWPE‑1 normal prostate epithelial cells. Different organic anion transporting peptide (OATP) inhibitors were utilized to explore the potential role of the OATP subtype, including the nonspecific OATP inhibitor bromosulfophthalein, the OATP1 inhibitor 17β‑estradiol, the selective OATP1B1 inhibitor rifampicin and the selective OATP1B3 inhibitor cholecystokinin octapeptide. NIRF dyes were also used for the simulated detection of circulating tumor cells and the rapid detection of prostate cancer in human prostate cancer tissues and prostate cancer xenografts in mouse models. The results revealed that the cancer‑specific uptake of these organic dyes in prostate cancer cells occurred primarily via OATP1B3. A strong NIRF signal was detected in prostate cancer tissues, but not in normal tissues that were stained with IR‑783. Prostate cancer cells were recognized with particular NIR fluorescence in isolated mononuclear cell mixtures. The results of the present study demonstrated that NIRF dye‑mediated imaging is a feasible and practicable method for prostate cancer detection, although further investigative studies are required before clinical translation.

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Selective dye uptake in DU-145, LNCaP, PC-3 prostate cancer cells. Only a weak near infrared fluorescence (red) was observed in RWPE-1 normal human prostate epithelial cells (magnification, ×200). The nuclei were stained with DAPI (blue).
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f1-mmr-11-02-0821: Selective dye uptake in DU-145, LNCaP, PC-3 prostate cancer cells. Only a weak near infrared fluorescence (red) was observed in RWPE-1 normal human prostate epithelial cells (magnification, ×200). The nuclei were stained with DAPI (blue).

Mentions: PC-3, DU-145, LNCaP human prostate cancer cells and RWPE-1 normal human prostate epithelial cells were selected for in vitro studies to evaluate the NIRF dye uptake and retention by prostate cancer cells. Three images were captured of the same visual field of the prepared slides using confocal microscopy, including transparent, DAPI and NIRF imaging patterns (Fig. 1). Selective uptake and accumulation of NIRF dyes was observed in all prostate cancer cells. However, there was only a weak NIRF signal detected in RWPE-1 cells. These results confirm the findings of a previous study that these dyes have a unique cancer targeting ability (5).


Near‑infrared fluorescence imaging of prostate cancer using heptamethine carbocyanine dyes.

Yuan J, Yi X, Yan F, Wang F, Qin W, Wu G, Yang X, Shao C, Chung LW - Mol Med Rep (2014)

Selective dye uptake in DU-145, LNCaP, PC-3 prostate cancer cells. Only a weak near infrared fluorescence (red) was observed in RWPE-1 normal human prostate epithelial cells (magnification, ×200). The nuclei were stained with DAPI (blue).
© Copyright Policy - open-access
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC4262509&req=5

f1-mmr-11-02-0821: Selective dye uptake in DU-145, LNCaP, PC-3 prostate cancer cells. Only a weak near infrared fluorescence (red) was observed in RWPE-1 normal human prostate epithelial cells (magnification, ×200). The nuclei were stained with DAPI (blue).
Mentions: PC-3, DU-145, LNCaP human prostate cancer cells and RWPE-1 normal human prostate epithelial cells were selected for in vitro studies to evaluate the NIRF dye uptake and retention by prostate cancer cells. Three images were captured of the same visual field of the prepared slides using confocal microscopy, including transparent, DAPI and NIRF imaging patterns (Fig. 1). Selective uptake and accumulation of NIRF dyes was observed in all prostate cancer cells. However, there was only a weak NIRF signal detected in RWPE-1 cells. These results confirm the findings of a previous study that these dyes have a unique cancer targeting ability (5).

Bottom Line: A previous study defined two organic polymethine cyanine dyes as ideal NIRF probes, IR‑783 and its derivative MHI‑148, which have excellent optical characteristics, superior biocompatibility and cancer targeting abilities.Different organic anion transporting peptide (OATP) inhibitors were utilized to explore the potential role of the OATP subtype, including the nonspecific OATP inhibitor bromosulfophthalein, the OATP1 inhibitor 17β‑estradiol, the selective OATP1B1 inhibitor rifampicin and the selective OATP1B3 inhibitor cholecystokinin octapeptide.The results of the present study demonstrated that NIRF dye‑mediated imaging is a feasible and practicable method for prostate cancer detection, although further investigative studies are required before clinical translation.

View Article: PubMed Central - PubMed

Affiliation: Department of Urology, Xijing Hospital, Fourth Military Medical University, Xi'an, Shaanxi 710032, P.R. China.

ABSTRACT
Near‑infrared fluorescence (NIRF) imaging is an attractive novel modality for the detection of cancer. A previous study defined two organic polymethine cyanine dyes as ideal NIRF probes, IR‑783 and its derivative MHI‑148, which have excellent optical characteristics, superior biocompatibility and cancer targeting abilities. To investigate the feasibility of NIRF dye‑mediated prostate cancer imaging, dye uptake and subcellular co‑localization were investigated in PC‑3, DU‑145 and LNCaP human prostate cancer cells and RWPE‑1 normal prostate epithelial cells. Different organic anion transporting peptide (OATP) inhibitors were utilized to explore the potential role of the OATP subtype, including the nonspecific OATP inhibitor bromosulfophthalein, the OATP1 inhibitor 17β‑estradiol, the selective OATP1B1 inhibitor rifampicin and the selective OATP1B3 inhibitor cholecystokinin octapeptide. NIRF dyes were also used for the simulated detection of circulating tumor cells and the rapid detection of prostate cancer in human prostate cancer tissues and prostate cancer xenografts in mouse models. The results revealed that the cancer‑specific uptake of these organic dyes in prostate cancer cells occurred primarily via OATP1B3. A strong NIRF signal was detected in prostate cancer tissues, but not in normal tissues that were stained with IR‑783. Prostate cancer cells were recognized with particular NIR fluorescence in isolated mononuclear cell mixtures. The results of the present study demonstrated that NIRF dye‑mediated imaging is a feasible and practicable method for prostate cancer detection, although further investigative studies are required before clinical translation.

Show MeSH
Related in: MedlinePlus