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Essential role of GATA3 in regulation of differentiation and cell proliferation in SK-N-SH neuroblastoma cells.

Peng H, Ke XX, Hu R, Yang L, Cui H, Wei Y - Mol Med Rep (2014)

Bottom Line: Neuroblastoma is a common solid malignant tumor of the sympathetic nervous system, which contributes to 15% of cancer‑related mortality in children.The results demonstrated that GATA3 is a prognostic marker for survival in patients with neuroblastoma, and that high‑level GATA3 expression is associated with increased self‑renewal and proliferation of neuroblastoma cells.Retinoic acid treatment led to GATA3 downregulation together with neuronal differentiation, suppression of cell proliferation and inhibition of tumorigenecity in neuroblastoma cells.

View Article: PubMed Central - PubMed

Affiliation: Laboratory of Cancer Biotherapy, State Key Laboratory of Biotherapy, West China Hospital, Sichuan University, Chengdu, Sichuan 610041, P.R. China.

ABSTRACT
Neuroblastoma is a common solid malignant tumor of the sympathetic nervous system, which contributes to 15% of cancer‑related mortality in children. The differentiation status of neuroblastoma is correlated with clinical outcome, and the induction of differentiation thus constitutes a therapeutic approach in this disease. However, the molecular mechanisms that control the differentiation of neuroblastoma remain poorly understood. The present study aimed to define whether GATA3 is involved in the differentiation of neuroblastoma cells. The results demonstrated that GATA3 is a prognostic marker for survival in patients with neuroblastoma, and that high‑level GATA3 expression is associated with increased self‑renewal and proliferation of neuroblastoma cells. Retinoic acid treatment led to GATA3 downregulation together with neuronal differentiation, suppression of cell proliferation and inhibition of tumorigenecity in neuroblastoma cells. These findings suggest that GATA3 is a key regulator of neuroblastoma differentiation, and provide a novel potential therapeutic strategy for the induction of neuroblastoma differentiation.

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Expression of GATA3 in various neuroblastoma cell lines. (A) Western blot analysis of GATA3 expression in eight neuroblastoma cell lines. (B) Western blot analysis of GATA3 expression in SK-N-SH, SK-N-SY5Y and SHEP1 cell lines. α-tubulin levels were used as a loading control.
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f2-mmr-11-02-0881: Expression of GATA3 in various neuroblastoma cell lines. (A) Western blot analysis of GATA3 expression in eight neuroblastoma cell lines. (B) Western blot analysis of GATA3 expression in SK-N-SH, SK-N-SY5Y and SHEP1 cell lines. α-tubulin levels were used as a loading control.

Mentions: The expression of GATA3 in various neuroblastoma cell lines was examined. GATA3 was found to be widely expressed in the majority of neuroblastoma cell lines (Fig. 2A), including BE (2)-C, IMR32, SK-N-DZ, SK-N-AS, and SK-N-BE, which are malignant cell lines. The expression of GATA3 was also investigated in the SHEP1 cell line, which is a benign neuroblastoma cell line with a highly differentiated status (31,32). The results indicated that GATA3 may be associated with the degree of neuroblastoma differentiation and the consequent prognosis. The SK-N-SH cells were a group of mixed cells which were isolated into SY5Y and SHEP1 in different conditions (33), and SY5Y is a type of malignant cell comparing with SHEP1 (32). As hypothesized, GATA3 expression was relatively high in the SY5Y and SK-N-SH cell lines, but there was no detectable expression in the SHEP1 cell line (Fig. 2B). This suggests that GATA3 may be used as a prognostic marker in neuroblastoma.


Essential role of GATA3 in regulation of differentiation and cell proliferation in SK-N-SH neuroblastoma cells.

Peng H, Ke XX, Hu R, Yang L, Cui H, Wei Y - Mol Med Rep (2014)

Expression of GATA3 in various neuroblastoma cell lines. (A) Western blot analysis of GATA3 expression in eight neuroblastoma cell lines. (B) Western blot analysis of GATA3 expression in SK-N-SH, SK-N-SY5Y and SHEP1 cell lines. α-tubulin levels were used as a loading control.
© Copyright Policy - open-access
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC4262502&req=5

f2-mmr-11-02-0881: Expression of GATA3 in various neuroblastoma cell lines. (A) Western blot analysis of GATA3 expression in eight neuroblastoma cell lines. (B) Western blot analysis of GATA3 expression in SK-N-SH, SK-N-SY5Y and SHEP1 cell lines. α-tubulin levels were used as a loading control.
Mentions: The expression of GATA3 in various neuroblastoma cell lines was examined. GATA3 was found to be widely expressed in the majority of neuroblastoma cell lines (Fig. 2A), including BE (2)-C, IMR32, SK-N-DZ, SK-N-AS, and SK-N-BE, which are malignant cell lines. The expression of GATA3 was also investigated in the SHEP1 cell line, which is a benign neuroblastoma cell line with a highly differentiated status (31,32). The results indicated that GATA3 may be associated with the degree of neuroblastoma differentiation and the consequent prognosis. The SK-N-SH cells were a group of mixed cells which were isolated into SY5Y and SHEP1 in different conditions (33), and SY5Y is a type of malignant cell comparing with SHEP1 (32). As hypothesized, GATA3 expression was relatively high in the SY5Y and SK-N-SH cell lines, but there was no detectable expression in the SHEP1 cell line (Fig. 2B). This suggests that GATA3 may be used as a prognostic marker in neuroblastoma.

Bottom Line: Neuroblastoma is a common solid malignant tumor of the sympathetic nervous system, which contributes to 15% of cancer‑related mortality in children.The results demonstrated that GATA3 is a prognostic marker for survival in patients with neuroblastoma, and that high‑level GATA3 expression is associated with increased self‑renewal and proliferation of neuroblastoma cells.Retinoic acid treatment led to GATA3 downregulation together with neuronal differentiation, suppression of cell proliferation and inhibition of tumorigenecity in neuroblastoma cells.

View Article: PubMed Central - PubMed

Affiliation: Laboratory of Cancer Biotherapy, State Key Laboratory of Biotherapy, West China Hospital, Sichuan University, Chengdu, Sichuan 610041, P.R. China.

ABSTRACT
Neuroblastoma is a common solid malignant tumor of the sympathetic nervous system, which contributes to 15% of cancer‑related mortality in children. The differentiation status of neuroblastoma is correlated with clinical outcome, and the induction of differentiation thus constitutes a therapeutic approach in this disease. However, the molecular mechanisms that control the differentiation of neuroblastoma remain poorly understood. The present study aimed to define whether GATA3 is involved in the differentiation of neuroblastoma cells. The results demonstrated that GATA3 is a prognostic marker for survival in patients with neuroblastoma, and that high‑level GATA3 expression is associated with increased self‑renewal and proliferation of neuroblastoma cells. Retinoic acid treatment led to GATA3 downregulation together with neuronal differentiation, suppression of cell proliferation and inhibition of tumorigenecity in neuroblastoma cells. These findings suggest that GATA3 is a key regulator of neuroblastoma differentiation, and provide a novel potential therapeutic strategy for the induction of neuroblastoma differentiation.

Show MeSH
Related in: MedlinePlus