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Essential role of GATA3 in regulation of differentiation and cell proliferation in SK-N-SH neuroblastoma cells.

Peng H, Ke XX, Hu R, Yang L, Cui H, Wei Y - Mol Med Rep (2014)

Bottom Line: Neuroblastoma is a common solid malignant tumor of the sympathetic nervous system, which contributes to 15% of cancer‑related mortality in children.The results demonstrated that GATA3 is a prognostic marker for survival in patients with neuroblastoma, and that high‑level GATA3 expression is associated with increased self‑renewal and proliferation of neuroblastoma cells.Retinoic acid treatment led to GATA3 downregulation together with neuronal differentiation, suppression of cell proliferation and inhibition of tumorigenecity in neuroblastoma cells.

View Article: PubMed Central - PubMed

Affiliation: Laboratory of Cancer Biotherapy, State Key Laboratory of Biotherapy, West China Hospital, Sichuan University, Chengdu, Sichuan 610041, P.R. China.

ABSTRACT
Neuroblastoma is a common solid malignant tumor of the sympathetic nervous system, which contributes to 15% of cancer‑related mortality in children. The differentiation status of neuroblastoma is correlated with clinical outcome, and the induction of differentiation thus constitutes a therapeutic approach in this disease. However, the molecular mechanisms that control the differentiation of neuroblastoma remain poorly understood. The present study aimed to define whether GATA3 is involved in the differentiation of neuroblastoma cells. The results demonstrated that GATA3 is a prognostic marker for survival in patients with neuroblastoma, and that high‑level GATA3 expression is associated with increased self‑renewal and proliferation of neuroblastoma cells. Retinoic acid treatment led to GATA3 downregulation together with neuronal differentiation, suppression of cell proliferation and inhibition of tumorigenecity in neuroblastoma cells. These findings suggest that GATA3 is a key regulator of neuroblastoma differentiation, and provide a novel potential therapeutic strategy for the induction of neuroblastoma differentiation.

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Related in: MedlinePlus

Association between GATA3 expression and survival in patients with neuroblastoma. (A) Kaplan-Meier analysis of progression-free survival for the Seeger database, with the log rank test P-value indicated. A cutoff value of 0.0365 was used to separate the patients into high and low GATA3 expression groups. (B) Box plot of GATA3 expression levels in tumors from groups of patients with good and poor prognoses.
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f1-mmr-11-02-0881: Association between GATA3 expression and survival in patients with neuroblastoma. (A) Kaplan-Meier analysis of progression-free survival for the Seeger database, with the log rank test P-value indicated. A cutoff value of 0.0365 was used to separate the patients into high and low GATA3 expression groups. (B) Box plot of GATA3 expression levels in tumors from groups of patients with good and poor prognoses.

Mentions: The correlation between GATA3 expression levels and prognosis in primary neuroblastoma was investigated using the Seeger microarray dataset, which is available from the online Oncogenomics database. This dataset includes a cohort of 102 neuroblastoma patients with metastatic tumors lacking MYCN amplification (30). Kaplan-Meier analysis of progression-free survival for the Seeger dataset showed that low GATA3 expression was associated with a good prognosis, whereas high GATA3 expression was associated with a poor outcome (Fig. 1A). Furthermore, a box plot of GATA3 expression levels in tumors from patients with either a good or a poor prognosis demonstrated the same result (Fig. 1B). This analysis indicated that GATA3 is a prognostic marker in neuroblastoma, which is independent of the status of MYCN amplification.


Essential role of GATA3 in regulation of differentiation and cell proliferation in SK-N-SH neuroblastoma cells.

Peng H, Ke XX, Hu R, Yang L, Cui H, Wei Y - Mol Med Rep (2014)

Association between GATA3 expression and survival in patients with neuroblastoma. (A) Kaplan-Meier analysis of progression-free survival for the Seeger database, with the log rank test P-value indicated. A cutoff value of 0.0365 was used to separate the patients into high and low GATA3 expression groups. (B) Box plot of GATA3 expression levels in tumors from groups of patients with good and poor prognoses.
© Copyright Policy - open-access
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC4262502&req=5

f1-mmr-11-02-0881: Association between GATA3 expression and survival in patients with neuroblastoma. (A) Kaplan-Meier analysis of progression-free survival for the Seeger database, with the log rank test P-value indicated. A cutoff value of 0.0365 was used to separate the patients into high and low GATA3 expression groups. (B) Box plot of GATA3 expression levels in tumors from groups of patients with good and poor prognoses.
Mentions: The correlation between GATA3 expression levels and prognosis in primary neuroblastoma was investigated using the Seeger microarray dataset, which is available from the online Oncogenomics database. This dataset includes a cohort of 102 neuroblastoma patients with metastatic tumors lacking MYCN amplification (30). Kaplan-Meier analysis of progression-free survival for the Seeger dataset showed that low GATA3 expression was associated with a good prognosis, whereas high GATA3 expression was associated with a poor outcome (Fig. 1A). Furthermore, a box plot of GATA3 expression levels in tumors from patients with either a good or a poor prognosis demonstrated the same result (Fig. 1B). This analysis indicated that GATA3 is a prognostic marker in neuroblastoma, which is independent of the status of MYCN amplification.

Bottom Line: Neuroblastoma is a common solid malignant tumor of the sympathetic nervous system, which contributes to 15% of cancer‑related mortality in children.The results demonstrated that GATA3 is a prognostic marker for survival in patients with neuroblastoma, and that high‑level GATA3 expression is associated with increased self‑renewal and proliferation of neuroblastoma cells.Retinoic acid treatment led to GATA3 downregulation together with neuronal differentiation, suppression of cell proliferation and inhibition of tumorigenecity in neuroblastoma cells.

View Article: PubMed Central - PubMed

Affiliation: Laboratory of Cancer Biotherapy, State Key Laboratory of Biotherapy, West China Hospital, Sichuan University, Chengdu, Sichuan 610041, P.R. China.

ABSTRACT
Neuroblastoma is a common solid malignant tumor of the sympathetic nervous system, which contributes to 15% of cancer‑related mortality in children. The differentiation status of neuroblastoma is correlated with clinical outcome, and the induction of differentiation thus constitutes a therapeutic approach in this disease. However, the molecular mechanisms that control the differentiation of neuroblastoma remain poorly understood. The present study aimed to define whether GATA3 is involved in the differentiation of neuroblastoma cells. The results demonstrated that GATA3 is a prognostic marker for survival in patients with neuroblastoma, and that high‑level GATA3 expression is associated with increased self‑renewal and proliferation of neuroblastoma cells. Retinoic acid treatment led to GATA3 downregulation together with neuronal differentiation, suppression of cell proliferation and inhibition of tumorigenecity in neuroblastoma cells. These findings suggest that GATA3 is a key regulator of neuroblastoma differentiation, and provide a novel potential therapeutic strategy for the induction of neuroblastoma differentiation.

Show MeSH
Related in: MedlinePlus