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Astragalus and Paeoniae radix rubra extract inhibits liver fibrosis by modulating the transforming growth factor‑β/Smad pathway in rats.

Huang W, Li L, Tian X, Yan J, Yang X, Wang X, Liao G, Qiu G - Mol Med Rep (2014)

Bottom Line: It has been previously demonstrated that Astragalus and Paeoniae radix rubra extract (APE) had a protective effect against liver fibrosis in mice.Moreover, APE (2.6 and 5.2 g/kg) decreased the elevation of TGF‑β1, α‑SMA, collagen I and collagen III expression, inhibited Smad2/3 phosphorylation as well as elevated the expression of the TGF‑β1 inhibitor Smad7.The mechanism of action of APE is hypothesized to proceed via scavenging free radicals, decreasing TGF‑β1 levels and blocking of the TGF‑β/Smad signaling pathway.

View Article: PubMed Central - PubMed

Affiliation: Department of Scientific Research, Xi'an Medical College, Xi'an, Shaanxi 710061, P.R. China.

ABSTRACT
It has been previously demonstrated that Astragalus and Paeoniae radix rubra extract (APE) had a protective effect against liver fibrosis in mice. The present study aimed to investigate the hepatoprotective effect of APE on CCl4‑induced hepatic fibrosis in rats. Liver fibrosis was induced in male Sprague‑Dawley rats by intraperitoneal injection of 50% CCl4 twice a week for eight weeks. Organ coefficients, serum aspartate aminotransferase (AST), alanine aminotransferase (ALT), hexadecenoic acid (HA), laminin (LN), procollagen type III (PCIII), hydroxyproline (Hyp), glutathione (GSH‑Px), malondialdehyde (MDA), superoxide dismutase (SOD) and transforming growth factor β1 (TGF‑β1) levels were measured in rats with hepatic fibrosis. Histopathological changes in affected livers were studied using hematoxylin‑eosin and Masson's trichrome staining. The expression of transforming growth factor‑β/Smad pathway proteins, α‑smooth muscle actin (α‑SMA), collagen I and collagen III was observed in fibrotic livers using western blot analysis. The present study observed significant reductions in serum levels of AST, ALT, HA, LN, PCIII and Hyp in APE‑treated (2.6 and 5.2 g/kg) rats, indicating the significant hepatoprotective effects of APE. Furthermore, the depletion of GSH‑Px and SOD, in addition to the accumulation of MDA in liver tissue was suppressed by APE (2.6 and 5.2 g/kg). Pathological assessment of CCl4‑induced fibrotic livers revealed a significant reduction of liver injury and development of hepatic fibrosis in rats treated with APE (2.6 and 5.2 g/kg). Moreover, APE (2.6 and 5.2 g/kg) decreased the elevation of TGF‑β1, α‑SMA, collagen I and collagen III expression, inhibited Smad2/3 phosphorylation as well as elevated the expression of the TGF‑β1 inhibitor Smad7. These results suggested that APE may protect against liver damage and inhibit the progression of CCl4‑induced hepatic fibrosis. The mechanism of action of APE is hypothesized to proceed via scavenging free radicals, decreasing TGF‑β1 levels and blocking of the TGF‑β/Smad signaling pathway.

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Body weight of rats from normal, model and drug-treated groups. The weights of rats in the model and CCl4 treament groups were markedly decreased when compared to those of the control group (P<0.05). When compared to the model group, APE (2.6 and 5.2 g/kg) administration significantly increased body weight in a dose-dependent manner (P<0.05). Low doses of APE (1.3 g/kg) and positive control (colchicine, 0.1 mg/kg) had no significant effect on the body weight of the animals (P>0.05). APE, Astragalus and Paeoniae radix rubra extract.
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f1-mmr-11-02-0805: Body weight of rats from normal, model and drug-treated groups. The weights of rats in the model and CCl4 treament groups were markedly decreased when compared to those of the control group (P<0.05). When compared to the model group, APE (2.6 and 5.2 g/kg) administration significantly increased body weight in a dose-dependent manner (P<0.05). Low doses of APE (1.3 g/kg) and positive control (colchicine, 0.1 mg/kg) had no significant effect on the body weight of the animals (P>0.05). APE, Astragalus and Paeoniae radix rubra extract.

Mentions: In comparison to the normal group, the weight of rats in the model group and the CCl4-treated groups was markedly decreased (P<0.05) (Fig. 1). The weight of animals receiving APE treatments (2.6 and 5.2 g/kg) significantly increased in a dose-dependent manner (P<0.05), compared to that of the model group. However, the lowest APE dose (1.3 g/kg) and colchicine (0.1 mg/kg), used as a positive control, had no significant effect on ameliorating CCl4-induced weight loss (P>0.05).


Astragalus and Paeoniae radix rubra extract inhibits liver fibrosis by modulating the transforming growth factor‑β/Smad pathway in rats.

Huang W, Li L, Tian X, Yan J, Yang X, Wang X, Liao G, Qiu G - Mol Med Rep (2014)

Body weight of rats from normal, model and drug-treated groups. The weights of rats in the model and CCl4 treament groups were markedly decreased when compared to those of the control group (P<0.05). When compared to the model group, APE (2.6 and 5.2 g/kg) administration significantly increased body weight in a dose-dependent manner (P<0.05). Low doses of APE (1.3 g/kg) and positive control (colchicine, 0.1 mg/kg) had no significant effect on the body weight of the animals (P>0.05). APE, Astragalus and Paeoniae radix rubra extract.
© Copyright Policy - open-access
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC4262501&req=5

f1-mmr-11-02-0805: Body weight of rats from normal, model and drug-treated groups. The weights of rats in the model and CCl4 treament groups were markedly decreased when compared to those of the control group (P<0.05). When compared to the model group, APE (2.6 and 5.2 g/kg) administration significantly increased body weight in a dose-dependent manner (P<0.05). Low doses of APE (1.3 g/kg) and positive control (colchicine, 0.1 mg/kg) had no significant effect on the body weight of the animals (P>0.05). APE, Astragalus and Paeoniae radix rubra extract.
Mentions: In comparison to the normal group, the weight of rats in the model group and the CCl4-treated groups was markedly decreased (P<0.05) (Fig. 1). The weight of animals receiving APE treatments (2.6 and 5.2 g/kg) significantly increased in a dose-dependent manner (P<0.05), compared to that of the model group. However, the lowest APE dose (1.3 g/kg) and colchicine (0.1 mg/kg), used as a positive control, had no significant effect on ameliorating CCl4-induced weight loss (P>0.05).

Bottom Line: It has been previously demonstrated that Astragalus and Paeoniae radix rubra extract (APE) had a protective effect against liver fibrosis in mice.Moreover, APE (2.6 and 5.2 g/kg) decreased the elevation of TGF‑β1, α‑SMA, collagen I and collagen III expression, inhibited Smad2/3 phosphorylation as well as elevated the expression of the TGF‑β1 inhibitor Smad7.The mechanism of action of APE is hypothesized to proceed via scavenging free radicals, decreasing TGF‑β1 levels and blocking of the TGF‑β/Smad signaling pathway.

View Article: PubMed Central - PubMed

Affiliation: Department of Scientific Research, Xi'an Medical College, Xi'an, Shaanxi 710061, P.R. China.

ABSTRACT
It has been previously demonstrated that Astragalus and Paeoniae radix rubra extract (APE) had a protective effect against liver fibrosis in mice. The present study aimed to investigate the hepatoprotective effect of APE on CCl4‑induced hepatic fibrosis in rats. Liver fibrosis was induced in male Sprague‑Dawley rats by intraperitoneal injection of 50% CCl4 twice a week for eight weeks. Organ coefficients, serum aspartate aminotransferase (AST), alanine aminotransferase (ALT), hexadecenoic acid (HA), laminin (LN), procollagen type III (PCIII), hydroxyproline (Hyp), glutathione (GSH‑Px), malondialdehyde (MDA), superoxide dismutase (SOD) and transforming growth factor β1 (TGF‑β1) levels were measured in rats with hepatic fibrosis. Histopathological changes in affected livers were studied using hematoxylin‑eosin and Masson's trichrome staining. The expression of transforming growth factor‑β/Smad pathway proteins, α‑smooth muscle actin (α‑SMA), collagen I and collagen III was observed in fibrotic livers using western blot analysis. The present study observed significant reductions in serum levels of AST, ALT, HA, LN, PCIII and Hyp in APE‑treated (2.6 and 5.2 g/kg) rats, indicating the significant hepatoprotective effects of APE. Furthermore, the depletion of GSH‑Px and SOD, in addition to the accumulation of MDA in liver tissue was suppressed by APE (2.6 and 5.2 g/kg). Pathological assessment of CCl4‑induced fibrotic livers revealed a significant reduction of liver injury and development of hepatic fibrosis in rats treated with APE (2.6 and 5.2 g/kg). Moreover, APE (2.6 and 5.2 g/kg) decreased the elevation of TGF‑β1, α‑SMA, collagen I and collagen III expression, inhibited Smad2/3 phosphorylation as well as elevated the expression of the TGF‑β1 inhibitor Smad7. These results suggested that APE may protect against liver damage and inhibit the progression of CCl4‑induced hepatic fibrosis. The mechanism of action of APE is hypothesized to proceed via scavenging free radicals, decreasing TGF‑β1 levels and blocking of the TGF‑β/Smad signaling pathway.

Show MeSH
Related in: MedlinePlus