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Self‑assembled monolayers of alkanethiolates on surface chemistry groups in osteosarcoma cells.

Deng YH, Li LH, He J, Li M, Zhang Y, Wang XM, Cui FZ, Xia H - Mol Med Rep (2014)

Bottom Line: The results revealed that different chemical surfaces convey different behaviors.The density of the different functional surfaces was confirmed by atomic force microscopy.In conclusion, the type of chemical group on a biomaterial is an important property for the growth of osteosarcoma cells; ‑NH2 and ‑COOH surfaces sustained visible cell adhesion and promoted cell growth.

View Article: PubMed Central - PubMed

Affiliation: Department of Orthopaedics Surgery, The First Clinical Medical College, Southern Medical University, Guangzhou, Guangdong 510515, P.R. China.

ABSTRACT
Cell biomedical behavior is influenced by a number of factors, and the extracellular matrix (ECM) of the cellular microenvironment affects certain cancer cells. In the current study, U‑2OS cells were cultured on gold surfaces modified with different terminal chemical groups [methyl (‑CH3), amino (‑NH2), hydroxyl (‑OH) and carboxyl (‑COOH)]. The results revealed that different chemical surfaces convey different behaviors. The density of the different functional surfaces was confirmed by atomic force microscopy. Cell morphology, proliferation rate and cell cycle were investigated using scanning electron microscopy, cell counting and flow cytometry. In conclusion, the type of chemical group on a biomaterial is an important property for the growth of osteosarcoma cells; ‑NH2 and ‑COOH surfaces sustained visible cell adhesion and promoted cell growth.

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Related in: MedlinePlus

Apoptosis and necrosis of U-2OS cultured on surfaces with different chemical functional groups after 24 h of culture. (A) -CH3; (B) -NH2; (C) -OH; and (D) -COOH groups.
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f7-mmr-11-02-0975: Apoptosis and necrosis of U-2OS cultured on surfaces with different chemical functional groups after 24 h of culture. (A) -CH3; (B) -NH2; (C) -OH; and (D) -COOH groups.

Mentions: U-2OS cells in different chemical groups were analyzed for apoptosis using Annexin V-PE and 7-AAD. Annexin V-PE was used to detect phosphatidylserine (PS) on the external membrane of apoptotic cells. 7-AAD, the cell impermeant dye, is used as an indicator of cell membrane structural integrity. As shown in Fig. 7, the -CH3 group caused ~7.6% apoptosis and ~12.5% necrosis, whereas the -NH2 group caused ~4.3% apoptosis and ~12.6% necrosis. The apoptosis rate showed the following trend: -CH3 > -COOH > -NH2 ≥ -OH. These results indicate that the -NH2 surface exhibits improved cell biocompatibility, and the -CH3 group may cause death by apoptosis and early apoptosis.


Self‑assembled monolayers of alkanethiolates on surface chemistry groups in osteosarcoma cells.

Deng YH, Li LH, He J, Li M, Zhang Y, Wang XM, Cui FZ, Xia H - Mol Med Rep (2014)

Apoptosis and necrosis of U-2OS cultured on surfaces with different chemical functional groups after 24 h of culture. (A) -CH3; (B) -NH2; (C) -OH; and (D) -COOH groups.
© Copyright Policy - open-access
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC4262499&req=5

f7-mmr-11-02-0975: Apoptosis and necrosis of U-2OS cultured on surfaces with different chemical functional groups after 24 h of culture. (A) -CH3; (B) -NH2; (C) -OH; and (D) -COOH groups.
Mentions: U-2OS cells in different chemical groups were analyzed for apoptosis using Annexin V-PE and 7-AAD. Annexin V-PE was used to detect phosphatidylserine (PS) on the external membrane of apoptotic cells. 7-AAD, the cell impermeant dye, is used as an indicator of cell membrane structural integrity. As shown in Fig. 7, the -CH3 group caused ~7.6% apoptosis and ~12.5% necrosis, whereas the -NH2 group caused ~4.3% apoptosis and ~12.6% necrosis. The apoptosis rate showed the following trend: -CH3 > -COOH > -NH2 ≥ -OH. These results indicate that the -NH2 surface exhibits improved cell biocompatibility, and the -CH3 group may cause death by apoptosis and early apoptosis.

Bottom Line: The results revealed that different chemical surfaces convey different behaviors.The density of the different functional surfaces was confirmed by atomic force microscopy.In conclusion, the type of chemical group on a biomaterial is an important property for the growth of osteosarcoma cells; ‑NH2 and ‑COOH surfaces sustained visible cell adhesion and promoted cell growth.

View Article: PubMed Central - PubMed

Affiliation: Department of Orthopaedics Surgery, The First Clinical Medical College, Southern Medical University, Guangzhou, Guangdong 510515, P.R. China.

ABSTRACT
Cell biomedical behavior is influenced by a number of factors, and the extracellular matrix (ECM) of the cellular microenvironment affects certain cancer cells. In the current study, U‑2OS cells were cultured on gold surfaces modified with different terminal chemical groups [methyl (‑CH3), amino (‑NH2), hydroxyl (‑OH) and carboxyl (‑COOH)]. The results revealed that different chemical surfaces convey different behaviors. The density of the different functional surfaces was confirmed by atomic force microscopy. Cell morphology, proliferation rate and cell cycle were investigated using scanning electron microscopy, cell counting and flow cytometry. In conclusion, the type of chemical group on a biomaterial is an important property for the growth of osteosarcoma cells; ‑NH2 and ‑COOH surfaces sustained visible cell adhesion and promoted cell growth.

Show MeSH
Related in: MedlinePlus