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Self‑assembled monolayers of alkanethiolates on surface chemistry groups in osteosarcoma cells.

Deng YH, Li LH, He J, Li M, Zhang Y, Wang XM, Cui FZ, Xia H - Mol Med Rep (2014)

Bottom Line: The results revealed that different chemical surfaces convey different behaviors.The density of the different functional surfaces was confirmed by atomic force microscopy.In conclusion, the type of chemical group on a biomaterial is an important property for the growth of osteosarcoma cells; ‑NH2 and ‑COOH surfaces sustained visible cell adhesion and promoted cell growth.

View Article: PubMed Central - PubMed

Affiliation: Department of Orthopaedics Surgery, The First Clinical Medical College, Southern Medical University, Guangzhou, Guangdong 510515, P.R. China.

ABSTRACT
Cell biomedical behavior is influenced by a number of factors, and the extracellular matrix (ECM) of the cellular microenvironment affects certain cancer cells. In the current study, U‑2OS cells were cultured on gold surfaces modified with different terminal chemical groups [methyl (‑CH3), amino (‑NH2), hydroxyl (‑OH) and carboxyl (‑COOH)]. The results revealed that different chemical surfaces convey different behaviors. The density of the different functional surfaces was confirmed by atomic force microscopy. Cell morphology, proliferation rate and cell cycle were investigated using scanning electron microscopy, cell counting and flow cytometry. In conclusion, the type of chemical group on a biomaterial is an important property for the growth of osteosarcoma cells; ‑NH2 and ‑COOH surfaces sustained visible cell adhesion and promoted cell growth.

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Lactate dehydrogenase (LDH) activity of U-2OS cells cultured on different chemical group-modified substrates at 24 h. Results are presented as the mean ± standard deviation of three independent experiments, each performed in triplicate.
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f6-mmr-11-02-0975: Lactate dehydrogenase (LDH) activity of U-2OS cells cultured on different chemical group-modified substrates at 24 h. Results are presented as the mean ± standard deviation of three independent experiments, each performed in triplicate.

Mentions: The present results indicated that the -CH3 group may interrupt the continuity of the cell membrane and subsequently lead to membrane breakdown and cytoplasm leakage. To test this assumption, the release of the cytoplasmic enzyme LDH by adherent cells was measured on chemical group-modified substrates. Assays of the LDH activities revealed the following trend: -CH3 > -OH ≥ -COOH > -NH2, which were in agreement with the MTT results, demonstrating the association between cell toxicity and the release of LDH enzymes (Fig. 6).


Self‑assembled monolayers of alkanethiolates on surface chemistry groups in osteosarcoma cells.

Deng YH, Li LH, He J, Li M, Zhang Y, Wang XM, Cui FZ, Xia H - Mol Med Rep (2014)

Lactate dehydrogenase (LDH) activity of U-2OS cells cultured on different chemical group-modified substrates at 24 h. Results are presented as the mean ± standard deviation of three independent experiments, each performed in triplicate.
© Copyright Policy - open-access
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC4262499&req=5

f6-mmr-11-02-0975: Lactate dehydrogenase (LDH) activity of U-2OS cells cultured on different chemical group-modified substrates at 24 h. Results are presented as the mean ± standard deviation of three independent experiments, each performed in triplicate.
Mentions: The present results indicated that the -CH3 group may interrupt the continuity of the cell membrane and subsequently lead to membrane breakdown and cytoplasm leakage. To test this assumption, the release of the cytoplasmic enzyme LDH by adherent cells was measured on chemical group-modified substrates. Assays of the LDH activities revealed the following trend: -CH3 > -OH ≥ -COOH > -NH2, which were in agreement with the MTT results, demonstrating the association between cell toxicity and the release of LDH enzymes (Fig. 6).

Bottom Line: The results revealed that different chemical surfaces convey different behaviors.The density of the different functional surfaces was confirmed by atomic force microscopy.In conclusion, the type of chemical group on a biomaterial is an important property for the growth of osteosarcoma cells; ‑NH2 and ‑COOH surfaces sustained visible cell adhesion and promoted cell growth.

View Article: PubMed Central - PubMed

Affiliation: Department of Orthopaedics Surgery, The First Clinical Medical College, Southern Medical University, Guangzhou, Guangdong 510515, P.R. China.

ABSTRACT
Cell biomedical behavior is influenced by a number of factors, and the extracellular matrix (ECM) of the cellular microenvironment affects certain cancer cells. In the current study, U‑2OS cells were cultured on gold surfaces modified with different terminal chemical groups [methyl (‑CH3), amino (‑NH2), hydroxyl (‑OH) and carboxyl (‑COOH)]. The results revealed that different chemical surfaces convey different behaviors. The density of the different functional surfaces was confirmed by atomic force microscopy. Cell morphology, proliferation rate and cell cycle were investigated using scanning electron microscopy, cell counting and flow cytometry. In conclusion, the type of chemical group on a biomaterial is an important property for the growth of osteosarcoma cells; ‑NH2 and ‑COOH surfaces sustained visible cell adhesion and promoted cell growth.

Show MeSH
Related in: MedlinePlus