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Self‑assembled monolayers of alkanethiolates on surface chemistry groups in osteosarcoma cells.

Deng YH, Li LH, He J, Li M, Zhang Y, Wang XM, Cui FZ, Xia H - Mol Med Rep (2014)

Bottom Line: The results revealed that different chemical surfaces convey different behaviors.The density of the different functional surfaces was confirmed by atomic force microscopy.In conclusion, the type of chemical group on a biomaterial is an important property for the growth of osteosarcoma cells; ‑NH2 and ‑COOH surfaces sustained visible cell adhesion and promoted cell growth.

View Article: PubMed Central - PubMed

Affiliation: Department of Orthopaedics Surgery, The First Clinical Medical College, Southern Medical University, Guangzhou, Guangdong 510515, P.R. China.

ABSTRACT
Cell biomedical behavior is influenced by a number of factors, and the extracellular matrix (ECM) of the cellular microenvironment affects certain cancer cells. In the current study, U‑2OS cells were cultured on gold surfaces modified with different terminal chemical groups [methyl (‑CH3), amino (‑NH2), hydroxyl (‑OH) and carboxyl (‑COOH)]. The results revealed that different chemical surfaces convey different behaviors. The density of the different functional surfaces was confirmed by atomic force microscopy. Cell morphology, proliferation rate and cell cycle were investigated using scanning electron microscopy, cell counting and flow cytometry. In conclusion, the type of chemical group on a biomaterial is an important property for the growth of osteosarcoma cells; ‑NH2 and ‑COOH surfaces sustained visible cell adhesion and promoted cell growth.

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MTT activity assay of U-2OS cultured on different chemical group-modified substrates at days 2, 4 and 6. Results represent the mean ± standard deviation of three independent cultures and determinations. *P<0.05,**P<0.01 compared with the same substrates at 4 days, as determined by one way analysis of variance. O.D., optical density.
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f4-mmr-11-02-0975: MTT activity assay of U-2OS cultured on different chemical group-modified substrates at days 2, 4 and 6. Results represent the mean ± standard deviation of three independent cultures and determinations. *P<0.05,**P<0.01 compared with the same substrates at 4 days, as determined by one way analysis of variance. O.D., optical density.

Mentions: The MTT results for the U-2OS cells cultured on the different chemical group-modified surfaces on days 2, 4 and 6 are shown in Fig. 4. The U-2OS cells on the different surfaces were uniformly seeded at the beginning of the experiment. From day 2 to day 6, there was a significant increase in the proliferation rate of U-2OS cells on the surfaces with the -CH3, -NH2, -OH and -COOH groups. However, the cells on the -CH3 surface had a low O.D. level compared with that of the other functional groups from Day 2 to Day 6. The cells in the control showed a similar moderate proliferation level to the -OH groups. It appears that the -COOH and -NH2 groups had a promoting effect on the proliferation of U-2OS cells in a longer culture period. However, the -CH3 group had a negative effect on U-2OS proliferation. Furthermore, the proliferation capacity of U-2OS on the different surfaces followed the trend: -COOH > -OH ≥ -NH2 >> -CH3.


Self‑assembled monolayers of alkanethiolates on surface chemistry groups in osteosarcoma cells.

Deng YH, Li LH, He J, Li M, Zhang Y, Wang XM, Cui FZ, Xia H - Mol Med Rep (2014)

MTT activity assay of U-2OS cultured on different chemical group-modified substrates at days 2, 4 and 6. Results represent the mean ± standard deviation of three independent cultures and determinations. *P<0.05,**P<0.01 compared with the same substrates at 4 days, as determined by one way analysis of variance. O.D., optical density.
© Copyright Policy - open-access
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC4262499&req=5

f4-mmr-11-02-0975: MTT activity assay of U-2OS cultured on different chemical group-modified substrates at days 2, 4 and 6. Results represent the mean ± standard deviation of three independent cultures and determinations. *P<0.05,**P<0.01 compared with the same substrates at 4 days, as determined by one way analysis of variance. O.D., optical density.
Mentions: The MTT results for the U-2OS cells cultured on the different chemical group-modified surfaces on days 2, 4 and 6 are shown in Fig. 4. The U-2OS cells on the different surfaces were uniformly seeded at the beginning of the experiment. From day 2 to day 6, there was a significant increase in the proliferation rate of U-2OS cells on the surfaces with the -CH3, -NH2, -OH and -COOH groups. However, the cells on the -CH3 surface had a low O.D. level compared with that of the other functional groups from Day 2 to Day 6. The cells in the control showed a similar moderate proliferation level to the -OH groups. It appears that the -COOH and -NH2 groups had a promoting effect on the proliferation of U-2OS cells in a longer culture period. However, the -CH3 group had a negative effect on U-2OS proliferation. Furthermore, the proliferation capacity of U-2OS on the different surfaces followed the trend: -COOH > -OH ≥ -NH2 >> -CH3.

Bottom Line: The results revealed that different chemical surfaces convey different behaviors.The density of the different functional surfaces was confirmed by atomic force microscopy.In conclusion, the type of chemical group on a biomaterial is an important property for the growth of osteosarcoma cells; ‑NH2 and ‑COOH surfaces sustained visible cell adhesion and promoted cell growth.

View Article: PubMed Central - PubMed

Affiliation: Department of Orthopaedics Surgery, The First Clinical Medical College, Southern Medical University, Guangzhou, Guangdong 510515, P.R. China.

ABSTRACT
Cell biomedical behavior is influenced by a number of factors, and the extracellular matrix (ECM) of the cellular microenvironment affects certain cancer cells. In the current study, U‑2OS cells were cultured on gold surfaces modified with different terminal chemical groups [methyl (‑CH3), amino (‑NH2), hydroxyl (‑OH) and carboxyl (‑COOH)]. The results revealed that different chemical surfaces convey different behaviors. The density of the different functional surfaces was confirmed by atomic force microscopy. Cell morphology, proliferation rate and cell cycle were investigated using scanning electron microscopy, cell counting and flow cytometry. In conclusion, the type of chemical group on a biomaterial is an important property for the growth of osteosarcoma cells; ‑NH2 and ‑COOH surfaces sustained visible cell adhesion and promoted cell growth.

Show MeSH
Related in: MedlinePlus