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Self‑assembled monolayers of alkanethiolates on surface chemistry groups in osteosarcoma cells.

Deng YH, Li LH, He J, Li M, Zhang Y, Wang XM, Cui FZ, Xia H - Mol Med Rep (2014)

Bottom Line: The results revealed that different chemical surfaces convey different behaviors.The density of the different functional surfaces was confirmed by atomic force microscopy.In conclusion, the type of chemical group on a biomaterial is an important property for the growth of osteosarcoma cells; ‑NH2 and ‑COOH surfaces sustained visible cell adhesion and promoted cell growth.

View Article: PubMed Central - PubMed

Affiliation: Department of Orthopaedics Surgery, The First Clinical Medical College, Southern Medical University, Guangzhou, Guangdong 510515, P.R. China.

ABSTRACT
Cell biomedical behavior is influenced by a number of factors, and the extracellular matrix (ECM) of the cellular microenvironment affects certain cancer cells. In the current study, U‑2OS cells were cultured on gold surfaces modified with different terminal chemical groups [methyl (‑CH3), amino (‑NH2), hydroxyl (‑OH) and carboxyl (‑COOH)]. The results revealed that different chemical surfaces convey different behaviors. The density of the different functional surfaces was confirmed by atomic force microscopy. Cell morphology, proliferation rate and cell cycle were investigated using scanning electron microscopy, cell counting and flow cytometry. In conclusion, the type of chemical group on a biomaterial is an important property for the growth of osteosarcoma cells; ‑NH2 and ‑COOH surfaces sustained visible cell adhesion and promoted cell growth.

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The scanning electron microscopy images of U-2OS cells cultured on surfaces with different chemical functional groups for 6 h. (A) -CH3; (B) -NH2; (C) -OH; and (D) -COOH surfaces. Scale bar, 20 μm.
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f3-mmr-11-02-0975: The scanning electron microscopy images of U-2OS cells cultured on surfaces with different chemical functional groups for 6 h. (A) -CH3; (B) -NH2; (C) -OH; and (D) -COOH surfaces. Scale bar, 20 μm.

Mentions: In order to further prove the different morphology changes, SEM (Fig. 3) was used. The results indicated that U-2OS cells cultured on the -OH and -COOH functional groups exhibit polygonal and oval morphology, those cultured on NH2 showed a spindle shape and cells cultured on -CH3 group were smaller and in a spherical shape, which was in accordance with the phase microscope results.


Self‑assembled monolayers of alkanethiolates on surface chemistry groups in osteosarcoma cells.

Deng YH, Li LH, He J, Li M, Zhang Y, Wang XM, Cui FZ, Xia H - Mol Med Rep (2014)

The scanning electron microscopy images of U-2OS cells cultured on surfaces with different chemical functional groups for 6 h. (A) -CH3; (B) -NH2; (C) -OH; and (D) -COOH surfaces. Scale bar, 20 μm.
© Copyright Policy - open-access
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC4262499&req=5

f3-mmr-11-02-0975: The scanning electron microscopy images of U-2OS cells cultured on surfaces with different chemical functional groups for 6 h. (A) -CH3; (B) -NH2; (C) -OH; and (D) -COOH surfaces. Scale bar, 20 μm.
Mentions: In order to further prove the different morphology changes, SEM (Fig. 3) was used. The results indicated that U-2OS cells cultured on the -OH and -COOH functional groups exhibit polygonal and oval morphology, those cultured on NH2 showed a spindle shape and cells cultured on -CH3 group were smaller and in a spherical shape, which was in accordance with the phase microscope results.

Bottom Line: The results revealed that different chemical surfaces convey different behaviors.The density of the different functional surfaces was confirmed by atomic force microscopy.In conclusion, the type of chemical group on a biomaterial is an important property for the growth of osteosarcoma cells; ‑NH2 and ‑COOH surfaces sustained visible cell adhesion and promoted cell growth.

View Article: PubMed Central - PubMed

Affiliation: Department of Orthopaedics Surgery, The First Clinical Medical College, Southern Medical University, Guangzhou, Guangdong 510515, P.R. China.

ABSTRACT
Cell biomedical behavior is influenced by a number of factors, and the extracellular matrix (ECM) of the cellular microenvironment affects certain cancer cells. In the current study, U‑2OS cells were cultured on gold surfaces modified with different terminal chemical groups [methyl (‑CH3), amino (‑NH2), hydroxyl (‑OH) and carboxyl (‑COOH)]. The results revealed that different chemical surfaces convey different behaviors. The density of the different functional surfaces was confirmed by atomic force microscopy. Cell morphology, proliferation rate and cell cycle were investigated using scanning electron microscopy, cell counting and flow cytometry. In conclusion, the type of chemical group on a biomaterial is an important property for the growth of osteosarcoma cells; ‑NH2 and ‑COOH surfaces sustained visible cell adhesion and promoted cell growth.

Show MeSH
Related in: MedlinePlus