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Self‑assembled monolayers of alkanethiolates on surface chemistry groups in osteosarcoma cells.

Deng YH, Li LH, He J, Li M, Zhang Y, Wang XM, Cui FZ, Xia H - Mol Med Rep (2014)

Bottom Line: The results revealed that different chemical surfaces convey different behaviors.The density of the different functional surfaces was confirmed by atomic force microscopy.In conclusion, the type of chemical group on a biomaterial is an important property for the growth of osteosarcoma cells; ‑NH2 and ‑COOH surfaces sustained visible cell adhesion and promoted cell growth.

View Article: PubMed Central - PubMed

Affiliation: Department of Orthopaedics Surgery, The First Clinical Medical College, Southern Medical University, Guangzhou, Guangdong 510515, P.R. China.

ABSTRACT
Cell biomedical behavior is influenced by a number of factors, and the extracellular matrix (ECM) of the cellular microenvironment affects certain cancer cells. In the current study, U‑2OS cells were cultured on gold surfaces modified with different terminal chemical groups [methyl (‑CH3), amino (‑NH2), hydroxyl (‑OH) and carboxyl (‑COOH)]. The results revealed that different chemical surfaces convey different behaviors. The density of the different functional surfaces was confirmed by atomic force microscopy. Cell morphology, proliferation rate and cell cycle were investigated using scanning electron microscopy, cell counting and flow cytometry. In conclusion, the type of chemical group on a biomaterial is an important property for the growth of osteosarcoma cells; ‑NH2 and ‑COOH surfaces sustained visible cell adhesion and promoted cell growth.

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Related in: MedlinePlus

The U-2OS cells cultured on surfaces with different chemical functional groups for 6 h (magnification, ×200). (A) -CH3 surface; (B) -NH2 surface; (C) -OH surface; (D) -COOH surface. (E) Control group. (F) Cell numbers on different functional groups in 3, 6 and 9 h. *P<0.05; **P<0.01 compared with the same substrates at 6 h.
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f2-mmr-11-02-0975: The U-2OS cells cultured on surfaces with different chemical functional groups for 6 h (magnification, ×200). (A) -CH3 surface; (B) -NH2 surface; (C) -OH surface; (D) -COOH surface. (E) Control group. (F) Cell numbers on different functional groups in 3, 6 and 9 h. *P<0.05; **P<0.01 compared with the same substrates at 6 h.

Mentions: Cell adhesion numbers on the different chemical surfaces after 3-, 6- and 9-h incubations are shown in Fig. 2. The adherent cell number of -CH3 group remained at the initial level. In contrast, the cell number on the -OH, -NH2 and -COOH surfaces was dramatically increased compared with that on -CH3 surface. The cell number on the -NH2 surface was higher than the other three groups during the incubation period. For the -NH2, -OH, -COOH groups, the number of cells increased accordingly. However, there were no clear differences between the -NH2 and -COOH groups. In addition, the adhesion number of the U-2OS cells followed the trend: -CH3 << -OH < -COOH ≈ -NH2.


Self‑assembled monolayers of alkanethiolates on surface chemistry groups in osteosarcoma cells.

Deng YH, Li LH, He J, Li M, Zhang Y, Wang XM, Cui FZ, Xia H - Mol Med Rep (2014)

The U-2OS cells cultured on surfaces with different chemical functional groups for 6 h (magnification, ×200). (A) -CH3 surface; (B) -NH2 surface; (C) -OH surface; (D) -COOH surface. (E) Control group. (F) Cell numbers on different functional groups in 3, 6 and 9 h. *P<0.05; **P<0.01 compared with the same substrates at 6 h.
© Copyright Policy - open-access
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC4262499&req=5

f2-mmr-11-02-0975: The U-2OS cells cultured on surfaces with different chemical functional groups for 6 h (magnification, ×200). (A) -CH3 surface; (B) -NH2 surface; (C) -OH surface; (D) -COOH surface. (E) Control group. (F) Cell numbers on different functional groups in 3, 6 and 9 h. *P<0.05; **P<0.01 compared with the same substrates at 6 h.
Mentions: Cell adhesion numbers on the different chemical surfaces after 3-, 6- and 9-h incubations are shown in Fig. 2. The adherent cell number of -CH3 group remained at the initial level. In contrast, the cell number on the -OH, -NH2 and -COOH surfaces was dramatically increased compared with that on -CH3 surface. The cell number on the -NH2 surface was higher than the other three groups during the incubation period. For the -NH2, -OH, -COOH groups, the number of cells increased accordingly. However, there were no clear differences between the -NH2 and -COOH groups. In addition, the adhesion number of the U-2OS cells followed the trend: -CH3 << -OH < -COOH ≈ -NH2.

Bottom Line: The results revealed that different chemical surfaces convey different behaviors.The density of the different functional surfaces was confirmed by atomic force microscopy.In conclusion, the type of chemical group on a biomaterial is an important property for the growth of osteosarcoma cells; ‑NH2 and ‑COOH surfaces sustained visible cell adhesion and promoted cell growth.

View Article: PubMed Central - PubMed

Affiliation: Department of Orthopaedics Surgery, The First Clinical Medical College, Southern Medical University, Guangzhou, Guangdong 510515, P.R. China.

ABSTRACT
Cell biomedical behavior is influenced by a number of factors, and the extracellular matrix (ECM) of the cellular microenvironment affects certain cancer cells. In the current study, U‑2OS cells were cultured on gold surfaces modified with different terminal chemical groups [methyl (‑CH3), amino (‑NH2), hydroxyl (‑OH) and carboxyl (‑COOH)]. The results revealed that different chemical surfaces convey different behaviors. The density of the different functional surfaces was confirmed by atomic force microscopy. Cell morphology, proliferation rate and cell cycle were investigated using scanning electron microscopy, cell counting and flow cytometry. In conclusion, the type of chemical group on a biomaterial is an important property for the growth of osteosarcoma cells; ‑NH2 and ‑COOH surfaces sustained visible cell adhesion and promoted cell growth.

Show MeSH
Related in: MedlinePlus