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Self‑assembled monolayers of alkanethiolates on surface chemistry groups in osteosarcoma cells.

Deng YH, Li LH, He J, Li M, Zhang Y, Wang XM, Cui FZ, Xia H - Mol Med Rep (2014)

Bottom Line: The results revealed that different chemical surfaces convey different behaviors.The density of the different functional surfaces was confirmed by atomic force microscopy.In conclusion, the type of chemical group on a biomaterial is an important property for the growth of osteosarcoma cells; ‑NH2 and ‑COOH surfaces sustained visible cell adhesion and promoted cell growth.

View Article: PubMed Central - PubMed

Affiliation: Department of Orthopaedics Surgery, The First Clinical Medical College, Southern Medical University, Guangzhou, Guangdong 510515, P.R. China.

ABSTRACT
Cell biomedical behavior is influenced by a number of factors, and the extracellular matrix (ECM) of the cellular microenvironment affects certain cancer cells. In the current study, U‑2OS cells were cultured on gold surfaces modified with different terminal chemical groups [methyl (‑CH3), amino (‑NH2), hydroxyl (‑OH) and carboxyl (‑COOH)]. The results revealed that different chemical surfaces convey different behaviors. The density of the different functional surfaces was confirmed by atomic force microscopy. Cell morphology, proliferation rate and cell cycle were investigated using scanning electron microscopy, cell counting and flow cytometry. In conclusion, the type of chemical group on a biomaterial is an important property for the growth of osteosarcoma cells; ‑NH2 and ‑COOH surfaces sustained visible cell adhesion and promoted cell growth.

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Self-assembled monolayer surfaces characterized by water contact angles. Results are presented as the mean ± standard deviation of six independent experiments, each performed in triplicate.
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f1-mmr-11-02-0975: Self-assembled monolayer surfaces characterized by water contact angles. Results are presented as the mean ± standard deviation of six independent experiments, each performed in triplicate.

Mentions: The results of contact angle measurements are shown in Fig. 1. Among these test surfaces, the -OH surface was the most hydrophilic, with a contact angle of 9.5°±1.2°. The -COOH surface had a slightly higher contact angle value of 18.3°±3.9°. The contact angle of the -NH2 surface was 59.7°±3.8°, although -NH2 was still classified as hydrophilic. Due to its non-polar nature, the -CH3 surface had the highest contact angle of 105.0°±9.1°, which was the most hydrophobic surface in these tests.


Self‑assembled monolayers of alkanethiolates on surface chemistry groups in osteosarcoma cells.

Deng YH, Li LH, He J, Li M, Zhang Y, Wang XM, Cui FZ, Xia H - Mol Med Rep (2014)

Self-assembled monolayer surfaces characterized by water contact angles. Results are presented as the mean ± standard deviation of six independent experiments, each performed in triplicate.
© Copyright Policy - open-access
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC4262499&req=5

f1-mmr-11-02-0975: Self-assembled monolayer surfaces characterized by water contact angles. Results are presented as the mean ± standard deviation of six independent experiments, each performed in triplicate.
Mentions: The results of contact angle measurements are shown in Fig. 1. Among these test surfaces, the -OH surface was the most hydrophilic, with a contact angle of 9.5°±1.2°. The -COOH surface had a slightly higher contact angle value of 18.3°±3.9°. The contact angle of the -NH2 surface was 59.7°±3.8°, although -NH2 was still classified as hydrophilic. Due to its non-polar nature, the -CH3 surface had the highest contact angle of 105.0°±9.1°, which was the most hydrophobic surface in these tests.

Bottom Line: The results revealed that different chemical surfaces convey different behaviors.The density of the different functional surfaces was confirmed by atomic force microscopy.In conclusion, the type of chemical group on a biomaterial is an important property for the growth of osteosarcoma cells; ‑NH2 and ‑COOH surfaces sustained visible cell adhesion and promoted cell growth.

View Article: PubMed Central - PubMed

Affiliation: Department of Orthopaedics Surgery, The First Clinical Medical College, Southern Medical University, Guangzhou, Guangdong 510515, P.R. China.

ABSTRACT
Cell biomedical behavior is influenced by a number of factors, and the extracellular matrix (ECM) of the cellular microenvironment affects certain cancer cells. In the current study, U‑2OS cells were cultured on gold surfaces modified with different terminal chemical groups [methyl (‑CH3), amino (‑NH2), hydroxyl (‑OH) and carboxyl (‑COOH)]. The results revealed that different chemical surfaces convey different behaviors. The density of the different functional surfaces was confirmed by atomic force microscopy. Cell morphology, proliferation rate and cell cycle were investigated using scanning electron microscopy, cell counting and flow cytometry. In conclusion, the type of chemical group on a biomaterial is an important property for the growth of osteosarcoma cells; ‑NH2 and ‑COOH surfaces sustained visible cell adhesion and promoted cell growth.

Show MeSH
Related in: MedlinePlus