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Role of cysteine‑rich angiogenic inducer 61 in fibroblast‑like synovial cell proliferation and invasion in rheumatoid arthritis.

Jie LG, Huang RY, Sun WF, Wei S, Chu YL, Huang QC, Du HY - Mol Med Rep (2014)

Bottom Line: The study found that the Cyr61 gene was highly expressed in the synovial cells from patients with RA compared with those from patients with OA.Conversely, overexpression of Cyr61 in normal FLS cells led to opposite effects.In conclusion, these results indicate that Cyr61 is capable of promoting RA‑FLS cell proliferation and invasion via the suppression of apoptosis and the regulation of MMP expression.

View Article: PubMed Central - PubMed

Affiliation: Department of Chinese Medicine, Guangzhou General Hospital of Guangzhou Command of PLA, Guangzhou, Guangdong 510010, P.R. China.

ABSTRACT
Cysteine‑rich angiogenic inducer 61 (Cyr61) is a novel molecule that has been shown to be increased in the synovial tissues of patients with rheumatoid arthritis (RA). The present study was conducted in order to investigate the role of Cyr61 in the pathogenesis of RA. A human genome‑wide gene assay was used to screen gene expression in synovial tissues obtained from four patients with RA and three patients with osteoarthritis (OA). To examine the role of Cyr61 in the phenotype of RA‑fibroblast‑like synovial (FLS) cells, Cyr61 expression in RA‑FLS cells was knocked down using small interfering RNA (siRNA). Normal FLS cells transduced with lentiviral vectors encoding Cyr61 cDNA were used to further explore the effects of this molecule on FLS cell apoptosis, proliferation and invasion. The study found that the Cyr61 gene was highly expressed in the synovial cells from patients with RA compared with those from patients with OA. Downregulation of Cyr61 by siRNA led to impaired cell proliferation and invasion. Furthermore, it decreased the levels of matrix metalloproteinase (MMP)‑3 and MMP‑13, and induced apoptosis in RA‑FLS cells. Conversely, overexpression of Cyr61 in normal FLS cells led to opposite effects. In conclusion, these results indicate that Cyr61 is capable of promoting RA‑FLS cell proliferation and invasion via the suppression of apoptosis and the regulation of MMP expression. Therefore, Cyr61 may be a good target molecule for the treatment and prevention of RA.

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Human genome-wide analysis of RA and OA synovial tissues. Differential expression of at least two fold (P<0.05) between RA and OA samples was found in 338 genes, one of which was Cyr61. **P<0.01, compared with OA synovial tissue. RA, rheumatoid arthritis; OA, osteoarthritis.
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f1-mmr-11-02-0917: Human genome-wide analysis of RA and OA synovial tissues. Differential expression of at least two fold (P<0.05) between RA and OA samples was found in 338 genes, one of which was Cyr61. **P<0.01, compared with OA synovial tissue. RA, rheumatoid arthritis; OA, osteoarthritis.

Mentions: A previous study (12) using immunohistochemistry, RT-qPCR and western blot analysis found that Cyr61 is overexpressed in synovial tissue and FLS cells from RA patients compared with samples from disease-free control subjects. To confirm this result, four synovial specimens from patients with RA and three samples from patients with OA were collected. Using a human genome-wide analysis, the present study found that the gene expression of Cyr61 in samples from patients with RA was 6.28-fold that of samples from patients with OA (P<0.01; Fig. 1). In accordance with this result, Zhang et al (12) found that the level of Cyr61 was higher in synovial fluid samples from RA patients than those from normal controls. These findings suggest that Cyr61 may be involved in the pathogenesis of RA.


Role of cysteine‑rich angiogenic inducer 61 in fibroblast‑like synovial cell proliferation and invasion in rheumatoid arthritis.

Jie LG, Huang RY, Sun WF, Wei S, Chu YL, Huang QC, Du HY - Mol Med Rep (2014)

Human genome-wide analysis of RA and OA synovial tissues. Differential expression of at least two fold (P<0.05) between RA and OA samples was found in 338 genes, one of which was Cyr61. **P<0.01, compared with OA synovial tissue. RA, rheumatoid arthritis; OA, osteoarthritis.
© Copyright Policy - open-access
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC4262486&req=5

f1-mmr-11-02-0917: Human genome-wide analysis of RA and OA synovial tissues. Differential expression of at least two fold (P<0.05) between RA and OA samples was found in 338 genes, one of which was Cyr61. **P<0.01, compared with OA synovial tissue. RA, rheumatoid arthritis; OA, osteoarthritis.
Mentions: A previous study (12) using immunohistochemistry, RT-qPCR and western blot analysis found that Cyr61 is overexpressed in synovial tissue and FLS cells from RA patients compared with samples from disease-free control subjects. To confirm this result, four synovial specimens from patients with RA and three samples from patients with OA were collected. Using a human genome-wide analysis, the present study found that the gene expression of Cyr61 in samples from patients with RA was 6.28-fold that of samples from patients with OA (P<0.01; Fig. 1). In accordance with this result, Zhang et al (12) found that the level of Cyr61 was higher in synovial fluid samples from RA patients than those from normal controls. These findings suggest that Cyr61 may be involved in the pathogenesis of RA.

Bottom Line: The study found that the Cyr61 gene was highly expressed in the synovial cells from patients with RA compared with those from patients with OA.Conversely, overexpression of Cyr61 in normal FLS cells led to opposite effects.In conclusion, these results indicate that Cyr61 is capable of promoting RA‑FLS cell proliferation and invasion via the suppression of apoptosis and the regulation of MMP expression.

View Article: PubMed Central - PubMed

Affiliation: Department of Chinese Medicine, Guangzhou General Hospital of Guangzhou Command of PLA, Guangzhou, Guangdong 510010, P.R. China.

ABSTRACT
Cysteine‑rich angiogenic inducer 61 (Cyr61) is a novel molecule that has been shown to be increased in the synovial tissues of patients with rheumatoid arthritis (RA). The present study was conducted in order to investigate the role of Cyr61 in the pathogenesis of RA. A human genome‑wide gene assay was used to screen gene expression in synovial tissues obtained from four patients with RA and three patients with osteoarthritis (OA). To examine the role of Cyr61 in the phenotype of RA‑fibroblast‑like synovial (FLS) cells, Cyr61 expression in RA‑FLS cells was knocked down using small interfering RNA (siRNA). Normal FLS cells transduced with lentiviral vectors encoding Cyr61 cDNA were used to further explore the effects of this molecule on FLS cell apoptosis, proliferation and invasion. The study found that the Cyr61 gene was highly expressed in the synovial cells from patients with RA compared with those from patients with OA. Downregulation of Cyr61 by siRNA led to impaired cell proliferation and invasion. Furthermore, it decreased the levels of matrix metalloproteinase (MMP)‑3 and MMP‑13, and induced apoptosis in RA‑FLS cells. Conversely, overexpression of Cyr61 in normal FLS cells led to opposite effects. In conclusion, these results indicate that Cyr61 is capable of promoting RA‑FLS cell proliferation and invasion via the suppression of apoptosis and the regulation of MMP expression. Therefore, Cyr61 may be a good target molecule for the treatment and prevention of RA.

Show MeSH
Related in: MedlinePlus