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A disintegrin and metalloproteinase 17 mRNA and protein expression in esophageal squamous cell carcinoma, as well as its clinicopathological factors and prognosis.

Liu HB, Yang QC, Shen Y, Zhu Y, Zhang XJ, Chen H - Mol Med Rep (2014)

Bottom Line: A log rank test and the Cox proportional hazards model were used for the esophageal cancer survival analysis.ADAM17 mRNA expression in esophageal squamous cell carcinoma was correlated with lymph node metastasis (P<0.01) and tumor, node and metastasis (TNM) staging (P<0.05), however, it was not correlated with gender, age or histological grade (P>0.05).ADAM17 protein expression and epidermal growth factor receptor (EGFR) protein expression were positively correlated (P<0.01).

View Article: PubMed Central - PubMed

Affiliation: Department of Pathology, The Second Affiliated Hospital of Nantong University, Nantong, Jiangsu 226001, P.R. China.

ABSTRACT
The aim of the present study was to explore a disintegrin and metalloproteinase 17 (ADAM17) mRNA and protein expression in esophageal squamous cell carcinoma and its association with clinicopathological factors and prognosis. Through semi‑quantitative reverse transcription polymerase chain reaction, the ADAM17 mRNA expression in 50 cases of esophageal squamous cell carcinoma and corresponding normal esophageal mucosa were detected. Using streptavidin peroxidase conjugated immunohistochemistry, ADAM17 protein levels were detected in 80 cases of esophageal squamous cell carcinoma and corresponding normal esophageal mucosa. A log rank test and the Cox proportional hazards model were used for the esophageal cancer survival analysis. ADAM17 mRNA expression levels in esophageal squamous cell carcinoma and corresponding normal esophageal mucosa were 0.937±0.241 and 0.225±0.077, respectively (P<0.01). ADAM17 mRNA expression in esophageal squamous cell carcinoma was correlated with lymph node metastasis (P<0.01) and tumor, node and metastasis (TNM) staging (P<0.05), however, it was not correlated with gender, age or histological grade (P>0.05). ADAM17 protein expression rates in esophageal squamous cell carcinoma and corresponding normal esophageal mucosa were 66.25 and 6.25% respectively, a difference that was statistically significant (P<0.01). In addition, ADAM17 protein expression in esophageal squamous cells was correlated with lymph node metastasis and TNM stage (P<0.05), while it was not correlated with gender, age or histological grade (P>0.05). ADAM17 protein expression and epidermal growth factor receptor (EGFR) protein expression were positively correlated (P<0.01). Lymph node metastasis, TNM stage, ADAM17 and EGFR protein expression may be used as independent prognostic indicators of esophageal squamous cell carcinoma (all P<0.05). ADAM17 mRNA and protein were highly expressed in esophageal squamous cell carcinoma; they have important roles in invasion and metastasis and a certain value in judging the prognosis of patients with esophageal squamous cell carcinoma.

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ADAM17 the Kaplan-Meier survival curves. ADAM17, a disintegrin and metalloproteinase 17.
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f5-mmr-11-02-0961: ADAM17 the Kaplan-Meier survival curves. ADAM17, a disintegrin and metalloproteinase 17.

Mentions: The follow-up data showed that the 1, 3 and 5-year survival rates in esophageal squamous cell carcinoma were 85.94, 34.38 and 11%, respectively. Through the follow-up data of esophageal squamous cell carcinoma patients, a univariate logrank survey showed that ADAM17 and EGFR were prognostic factors affecting esophageal squamous cell carcinoma (All P<0.05, Table IV). Further ADAM17 Kaplan-Meier survival curves showed that the survival rate in the ADAM17 positive group was significantly lower than those in ADAM17 negative group (P<0.01) (Fig. 5). The multivariate Cox proportional hazards model analysis showed that ADAM17, EGFR, lymph node metastasis and TNM stage all had independent prognostic significance (all P<0.05, Table V). This indicates that ADAM17 and EGFR expression may increase the risk of esophageal cancer mortality.


A disintegrin and metalloproteinase 17 mRNA and protein expression in esophageal squamous cell carcinoma, as well as its clinicopathological factors and prognosis.

Liu HB, Yang QC, Shen Y, Zhu Y, Zhang XJ, Chen H - Mol Med Rep (2014)

ADAM17 the Kaplan-Meier survival curves. ADAM17, a disintegrin and metalloproteinase 17.
© Copyright Policy - open-access
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC4262481&req=5

f5-mmr-11-02-0961: ADAM17 the Kaplan-Meier survival curves. ADAM17, a disintegrin and metalloproteinase 17.
Mentions: The follow-up data showed that the 1, 3 and 5-year survival rates in esophageal squamous cell carcinoma were 85.94, 34.38 and 11%, respectively. Through the follow-up data of esophageal squamous cell carcinoma patients, a univariate logrank survey showed that ADAM17 and EGFR were prognostic factors affecting esophageal squamous cell carcinoma (All P<0.05, Table IV). Further ADAM17 Kaplan-Meier survival curves showed that the survival rate in the ADAM17 positive group was significantly lower than those in ADAM17 negative group (P<0.01) (Fig. 5). The multivariate Cox proportional hazards model analysis showed that ADAM17, EGFR, lymph node metastasis and TNM stage all had independent prognostic significance (all P<0.05, Table V). This indicates that ADAM17 and EGFR expression may increase the risk of esophageal cancer mortality.

Bottom Line: A log rank test and the Cox proportional hazards model were used for the esophageal cancer survival analysis.ADAM17 mRNA expression in esophageal squamous cell carcinoma was correlated with lymph node metastasis (P<0.01) and tumor, node and metastasis (TNM) staging (P<0.05), however, it was not correlated with gender, age or histological grade (P>0.05).ADAM17 protein expression and epidermal growth factor receptor (EGFR) protein expression were positively correlated (P<0.01).

View Article: PubMed Central - PubMed

Affiliation: Department of Pathology, The Second Affiliated Hospital of Nantong University, Nantong, Jiangsu 226001, P.R. China.

ABSTRACT
The aim of the present study was to explore a disintegrin and metalloproteinase 17 (ADAM17) mRNA and protein expression in esophageal squamous cell carcinoma and its association with clinicopathological factors and prognosis. Through semi‑quantitative reverse transcription polymerase chain reaction, the ADAM17 mRNA expression in 50 cases of esophageal squamous cell carcinoma and corresponding normal esophageal mucosa were detected. Using streptavidin peroxidase conjugated immunohistochemistry, ADAM17 protein levels were detected in 80 cases of esophageal squamous cell carcinoma and corresponding normal esophageal mucosa. A log rank test and the Cox proportional hazards model were used for the esophageal cancer survival analysis. ADAM17 mRNA expression levels in esophageal squamous cell carcinoma and corresponding normal esophageal mucosa were 0.937±0.241 and 0.225±0.077, respectively (P<0.01). ADAM17 mRNA expression in esophageal squamous cell carcinoma was correlated with lymph node metastasis (P<0.01) and tumor, node and metastasis (TNM) staging (P<0.05), however, it was not correlated with gender, age or histological grade (P>0.05). ADAM17 protein expression rates in esophageal squamous cell carcinoma and corresponding normal esophageal mucosa were 66.25 and 6.25% respectively, a difference that was statistically significant (P<0.01). In addition, ADAM17 protein expression in esophageal squamous cells was correlated with lymph node metastasis and TNM stage (P<0.05), while it was not correlated with gender, age or histological grade (P>0.05). ADAM17 protein expression and epidermal growth factor receptor (EGFR) protein expression were positively correlated (P<0.01). Lymph node metastasis, TNM stage, ADAM17 and EGFR protein expression may be used as independent prognostic indicators of esophageal squamous cell carcinoma (all P<0.05). ADAM17 mRNA and protein were highly expressed in esophageal squamous cell carcinoma; they have important roles in invasion and metastasis and a certain value in judging the prognosis of patients with esophageal squamous cell carcinoma.

Show MeSH
Related in: MedlinePlus