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A disintegrin and metalloproteinase 17 mRNA and protein expression in esophageal squamous cell carcinoma, as well as its clinicopathological factors and prognosis.

Liu HB, Yang QC, Shen Y, Zhu Y, Zhang XJ, Chen H - Mol Med Rep (2014)

Bottom Line: A log rank test and the Cox proportional hazards model were used for the esophageal cancer survival analysis.ADAM17 mRNA expression in esophageal squamous cell carcinoma was correlated with lymph node metastasis (P<0.01) and tumor, node and metastasis (TNM) staging (P<0.05), however, it was not correlated with gender, age or histological grade (P>0.05).ADAM17 protein expression and epidermal growth factor receptor (EGFR) protein expression were positively correlated (P<0.01).

View Article: PubMed Central - PubMed

Affiliation: Department of Pathology, The Second Affiliated Hospital of Nantong University, Nantong, Jiangsu 226001, P.R. China.

ABSTRACT
The aim of the present study was to explore a disintegrin and metalloproteinase 17 (ADAM17) mRNA and protein expression in esophageal squamous cell carcinoma and its association with clinicopathological factors and prognosis. Through semi‑quantitative reverse transcription polymerase chain reaction, the ADAM17 mRNA expression in 50 cases of esophageal squamous cell carcinoma and corresponding normal esophageal mucosa were detected. Using streptavidin peroxidase conjugated immunohistochemistry, ADAM17 protein levels were detected in 80 cases of esophageal squamous cell carcinoma and corresponding normal esophageal mucosa. A log rank test and the Cox proportional hazards model were used for the esophageal cancer survival analysis. ADAM17 mRNA expression levels in esophageal squamous cell carcinoma and corresponding normal esophageal mucosa were 0.937±0.241 and 0.225±0.077, respectively (P<0.01). ADAM17 mRNA expression in esophageal squamous cell carcinoma was correlated with lymph node metastasis (P<0.01) and tumor, node and metastasis (TNM) staging (P<0.05), however, it was not correlated with gender, age or histological grade (P>0.05). ADAM17 protein expression rates in esophageal squamous cell carcinoma and corresponding normal esophageal mucosa were 66.25 and 6.25% respectively, a difference that was statistically significant (P<0.01). In addition, ADAM17 protein expression in esophageal squamous cells was correlated with lymph node metastasis and TNM stage (P<0.05), while it was not correlated with gender, age or histological grade (P>0.05). ADAM17 protein expression and epidermal growth factor receptor (EGFR) protein expression were positively correlated (P<0.01). Lymph node metastasis, TNM stage, ADAM17 and EGFR protein expression may be used as independent prognostic indicators of esophageal squamous cell carcinoma (all P<0.05). ADAM17 mRNA and protein were highly expressed in esophageal squamous cell carcinoma; they have important roles in invasion and metastasis and a certain value in judging the prognosis of patients with esophageal squamous cell carcinoma.

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ADAM17 mRNA reverse transcription polymerase chain reaction amplification results electrophoresis. Lanes: M, DNA Marker; 1 and 2, histological grade I; 3 and 4, histological grade II; 5 and 6, histological grade III.
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f3-mmr-11-02-0961: ADAM17 mRNA reverse transcription polymerase chain reaction amplification results electrophoresis. Lanes: M, DNA Marker; 1 and 2, histological grade I; 3 and 4, histological grade II; 5 and 6, histological grade III.

Mentions: The length of the reference β-actin gene fragment was 218 bp. The length of ADAM17 gene fragment was 440 bp. The normal esophageal mucosa and esophageal squamous cell carcinoma had varying intensities of expression (Fig. 1). In the 50 cases of esophageal squamous cell carcinoma, the ratio of ADAM17 mRNA expression to that of β-actin was increased to 0.937±0.241 (Table I), compared with the ratio of expressions in normal esophageal mucosa, which was 0.225 ± 0.077 (P<0.01). As shown in Table II, in the 50 cases of esophageal squamous cell carcinoma, ADAM17 mRNA expression was significantly higher in the lymph node metastasis group than that of the lymph node-negative group (P<0.01) (Fig. 2). In the TNM stages, the ADAM17 mRNA expression of stage I + II patients was significantly different to that of stage III + IV patients (P<0.05). Esophageal squamous cell histology revealed that the levels of ADAM17 mRNA expression in stage I, II and III was increased, however, no significant difference was determined (P>0.05) (Fig. 3). In addition, ADAM17 mRNA expression had no correlation with other clinicopathological factors, including gender and age (P>0.05).


A disintegrin and metalloproteinase 17 mRNA and protein expression in esophageal squamous cell carcinoma, as well as its clinicopathological factors and prognosis.

Liu HB, Yang QC, Shen Y, Zhu Y, Zhang XJ, Chen H - Mol Med Rep (2014)

ADAM17 mRNA reverse transcription polymerase chain reaction amplification results electrophoresis. Lanes: M, DNA Marker; 1 and 2, histological grade I; 3 and 4, histological grade II; 5 and 6, histological grade III.
© Copyright Policy - open-access
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC4262481&req=5

f3-mmr-11-02-0961: ADAM17 mRNA reverse transcription polymerase chain reaction amplification results electrophoresis. Lanes: M, DNA Marker; 1 and 2, histological grade I; 3 and 4, histological grade II; 5 and 6, histological grade III.
Mentions: The length of the reference β-actin gene fragment was 218 bp. The length of ADAM17 gene fragment was 440 bp. The normal esophageal mucosa and esophageal squamous cell carcinoma had varying intensities of expression (Fig. 1). In the 50 cases of esophageal squamous cell carcinoma, the ratio of ADAM17 mRNA expression to that of β-actin was increased to 0.937±0.241 (Table I), compared with the ratio of expressions in normal esophageal mucosa, which was 0.225 ± 0.077 (P<0.01). As shown in Table II, in the 50 cases of esophageal squamous cell carcinoma, ADAM17 mRNA expression was significantly higher in the lymph node metastasis group than that of the lymph node-negative group (P<0.01) (Fig. 2). In the TNM stages, the ADAM17 mRNA expression of stage I + II patients was significantly different to that of stage III + IV patients (P<0.05). Esophageal squamous cell histology revealed that the levels of ADAM17 mRNA expression in stage I, II and III was increased, however, no significant difference was determined (P>0.05) (Fig. 3). In addition, ADAM17 mRNA expression had no correlation with other clinicopathological factors, including gender and age (P>0.05).

Bottom Line: A log rank test and the Cox proportional hazards model were used for the esophageal cancer survival analysis.ADAM17 mRNA expression in esophageal squamous cell carcinoma was correlated with lymph node metastasis (P<0.01) and tumor, node and metastasis (TNM) staging (P<0.05), however, it was not correlated with gender, age or histological grade (P>0.05).ADAM17 protein expression and epidermal growth factor receptor (EGFR) protein expression were positively correlated (P<0.01).

View Article: PubMed Central - PubMed

Affiliation: Department of Pathology, The Second Affiliated Hospital of Nantong University, Nantong, Jiangsu 226001, P.R. China.

ABSTRACT
The aim of the present study was to explore a disintegrin and metalloproteinase 17 (ADAM17) mRNA and protein expression in esophageal squamous cell carcinoma and its association with clinicopathological factors and prognosis. Through semi‑quantitative reverse transcription polymerase chain reaction, the ADAM17 mRNA expression in 50 cases of esophageal squamous cell carcinoma and corresponding normal esophageal mucosa were detected. Using streptavidin peroxidase conjugated immunohistochemistry, ADAM17 protein levels were detected in 80 cases of esophageal squamous cell carcinoma and corresponding normal esophageal mucosa. A log rank test and the Cox proportional hazards model were used for the esophageal cancer survival analysis. ADAM17 mRNA expression levels in esophageal squamous cell carcinoma and corresponding normal esophageal mucosa were 0.937±0.241 and 0.225±0.077, respectively (P<0.01). ADAM17 mRNA expression in esophageal squamous cell carcinoma was correlated with lymph node metastasis (P<0.01) and tumor, node and metastasis (TNM) staging (P<0.05), however, it was not correlated with gender, age or histological grade (P>0.05). ADAM17 protein expression rates in esophageal squamous cell carcinoma and corresponding normal esophageal mucosa were 66.25 and 6.25% respectively, a difference that was statistically significant (P<0.01). In addition, ADAM17 protein expression in esophageal squamous cells was correlated with lymph node metastasis and TNM stage (P<0.05), while it was not correlated with gender, age or histological grade (P>0.05). ADAM17 protein expression and epidermal growth factor receptor (EGFR) protein expression were positively correlated (P<0.01). Lymph node metastasis, TNM stage, ADAM17 and EGFR protein expression may be used as independent prognostic indicators of esophageal squamous cell carcinoma (all P<0.05). ADAM17 mRNA and protein were highly expressed in esophageal squamous cell carcinoma; they have important roles in invasion and metastasis and a certain value in judging the prognosis of patients with esophageal squamous cell carcinoma.

Show MeSH
Related in: MedlinePlus