Limits...
Arsenic sulfide as a potential anti‑cancer drug.

Ding W, Zhang L, Kim S, Tian W, Tong Y, Liu J, Ma Y, Chen S - Mol Med Rep (2014)

Bottom Line: Arsenic sulfide (As4S4) is the main component of realgar, which is widely used in traditional Chinese medicine.Additionally, As4S4 was observed to induce apoptosis (including morphological changes and an enhanced sub‑G1 population), which was accompanied by the activation of caspase‑3 and ‑9.These results suggest that As4S4 possesses potent in vitro and in vivo antitumor activity via the induction of cell apoptosis.

View Article: PubMed Central - PubMed

Affiliation: Department of Oncology, Xinhua Hospital Affiliated to Shanghai Jiao Tong University School of Medicine, Shanghai 200092, P.R. China.

ABSTRACT
Arsenic sulfide (As4S4) is the main component of realgar, which is widely used in traditional Chinese medicine. Previous studies have shown the beneficial effects of As4S4 in the treatment of hematological malignant diseases, however, its effects on solid tumors have yet to be fully elucidated. The current study aimed to explore the anti‑cancer effect and the mechanism of As4S4 on solid tumors in vitro and in vivo. Cells from four human solid tumor cell lines, including the MKN45 gastric cancer cell line, the A375 malignant melanoma cell line, the 8898 pancreatic carcinoma cell line and the HepG2 hepatocellular carcinoma cell line, were treated with As4S4 in vitro, using the L02 embryonic liver cells as a control. The efficacy of As4S4 was assessed in vivo using mice implanted with Lewis lung carcinoma cells. The results of the current study demonstrated that As4S4 significantly inhibited the proliferation of solid tumor cells in a dose‑ and time‑dependent manner, but produced a less pronounced effect on L02 cells. Additionally, As4S4 was observed to induce apoptosis (including morphological changes and an enhanced sub‑G1 population), which was accompanied by the activation of caspase‑3 and ‑9. Furthermore, treatment with As4S4 significantly inhibited the growth of implanted tumors in mice. These results suggest that As4S4 possesses potent in vitro and in vivo antitumor activity via the induction of cell apoptosis.

Show MeSH

Related in: MedlinePlus

As4S4 inhibited tumor growth and elevated the levels of IL-2 in the tumor-bearing C57BL/6 mice. (A) Tumor weight reduced significantly following treatment with 60 mg/kg (H) of As4S4, but the reduction observed was not significant with 30 mg/kg (L). (B) Levels of IL-2 were significantly elevated in the treatment groups compared with the NC group. Data are expressed as the mean ± standard deviation; *P<0.05, **P<0.01 and ***P<0.001 vs. NC. As4S4, arsenic sulfide; IL-2, interleukin 2; L, low; H, high; NC, negative control; CTX, cyclophosphamide.
© Copyright Policy - open-access
Related In: Results  -  Collection

License
getmorefigures.php?uid=PMC4262477&req=5

f5-mmr-11-02-0968: As4S4 inhibited tumor growth and elevated the levels of IL-2 in the tumor-bearing C57BL/6 mice. (A) Tumor weight reduced significantly following treatment with 60 mg/kg (H) of As4S4, but the reduction observed was not significant with 30 mg/kg (L). (B) Levels of IL-2 were significantly elevated in the treatment groups compared with the NC group. Data are expressed as the mean ± standard deviation; *P<0.05, **P<0.01 and ***P<0.001 vs. NC. As4S4, arsenic sulfide; IL-2, interleukin 2; L, low; H, high; NC, negative control; CTX, cyclophosphamide.

Mentions: Mouse lung cancer LLC cells were implanted in C57BL/6 mice. Following treatment with As4S4 for eight days, the suppression of tumor growth was observed (Fig. 5A). The inhibition ratios of the low-dose group (As4S4, 30 mg/kg) and high-dose group (As4S4, 60 mg/kg) were 26.45 and 47.93%, respectively (Table II). The high dosage exhibited a greater anticancer effect than the low dosage, and produced a significantly reduced tumor weight compared with that of the NC group. In addition, As4S4 treatment did not significantly alter the body weight of the mice (data not shown). Subsequent to treatment with As4S4, the concentrations of IL-2 in the treatment groups were higher compared with the NC group (Fig. 5B). These results demonstrate that As4S4 is able to suppress tumor growth.


Arsenic sulfide as a potential anti‑cancer drug.

Ding W, Zhang L, Kim S, Tian W, Tong Y, Liu J, Ma Y, Chen S - Mol Med Rep (2014)

As4S4 inhibited tumor growth and elevated the levels of IL-2 in the tumor-bearing C57BL/6 mice. (A) Tumor weight reduced significantly following treatment with 60 mg/kg (H) of As4S4, but the reduction observed was not significant with 30 mg/kg (L). (B) Levels of IL-2 were significantly elevated in the treatment groups compared with the NC group. Data are expressed as the mean ± standard deviation; *P<0.05, **P<0.01 and ***P<0.001 vs. NC. As4S4, arsenic sulfide; IL-2, interleukin 2; L, low; H, high; NC, negative control; CTX, cyclophosphamide.
© Copyright Policy - open-access
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC4262477&req=5

f5-mmr-11-02-0968: As4S4 inhibited tumor growth and elevated the levels of IL-2 in the tumor-bearing C57BL/6 mice. (A) Tumor weight reduced significantly following treatment with 60 mg/kg (H) of As4S4, but the reduction observed was not significant with 30 mg/kg (L). (B) Levels of IL-2 were significantly elevated in the treatment groups compared with the NC group. Data are expressed as the mean ± standard deviation; *P<0.05, **P<0.01 and ***P<0.001 vs. NC. As4S4, arsenic sulfide; IL-2, interleukin 2; L, low; H, high; NC, negative control; CTX, cyclophosphamide.
Mentions: Mouse lung cancer LLC cells were implanted in C57BL/6 mice. Following treatment with As4S4 for eight days, the suppression of tumor growth was observed (Fig. 5A). The inhibition ratios of the low-dose group (As4S4, 30 mg/kg) and high-dose group (As4S4, 60 mg/kg) were 26.45 and 47.93%, respectively (Table II). The high dosage exhibited a greater anticancer effect than the low dosage, and produced a significantly reduced tumor weight compared with that of the NC group. In addition, As4S4 treatment did not significantly alter the body weight of the mice (data not shown). Subsequent to treatment with As4S4, the concentrations of IL-2 in the treatment groups were higher compared with the NC group (Fig. 5B). These results demonstrate that As4S4 is able to suppress tumor growth.

Bottom Line: Arsenic sulfide (As4S4) is the main component of realgar, which is widely used in traditional Chinese medicine.Additionally, As4S4 was observed to induce apoptosis (including morphological changes and an enhanced sub‑G1 population), which was accompanied by the activation of caspase‑3 and ‑9.These results suggest that As4S4 possesses potent in vitro and in vivo antitumor activity via the induction of cell apoptosis.

View Article: PubMed Central - PubMed

Affiliation: Department of Oncology, Xinhua Hospital Affiliated to Shanghai Jiao Tong University School of Medicine, Shanghai 200092, P.R. China.

ABSTRACT
Arsenic sulfide (As4S4) is the main component of realgar, which is widely used in traditional Chinese medicine. Previous studies have shown the beneficial effects of As4S4 in the treatment of hematological malignant diseases, however, its effects on solid tumors have yet to be fully elucidated. The current study aimed to explore the anti‑cancer effect and the mechanism of As4S4 on solid tumors in vitro and in vivo. Cells from four human solid tumor cell lines, including the MKN45 gastric cancer cell line, the A375 malignant melanoma cell line, the 8898 pancreatic carcinoma cell line and the HepG2 hepatocellular carcinoma cell line, were treated with As4S4 in vitro, using the L02 embryonic liver cells as a control. The efficacy of As4S4 was assessed in vivo using mice implanted with Lewis lung carcinoma cells. The results of the current study demonstrated that As4S4 significantly inhibited the proliferation of solid tumor cells in a dose‑ and time‑dependent manner, but produced a less pronounced effect on L02 cells. Additionally, As4S4 was observed to induce apoptosis (including morphological changes and an enhanced sub‑G1 population), which was accompanied by the activation of caspase‑3 and ‑9. Furthermore, treatment with As4S4 significantly inhibited the growth of implanted tumors in mice. These results suggest that As4S4 possesses potent in vitro and in vivo antitumor activity via the induction of cell apoptosis.

Show MeSH
Related in: MedlinePlus