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Chemical Composition and Anti-Inflammatory, Cytotoxic and Antioxidant Activities of Essential Oil from Leaves of Mentha piperita Grown in China.

Sun Z, Wang H, Wang J, Zhou L, Yang P - PLoS ONE (2014)

Bottom Line: It could also effectively inhibit nitric oxide (NO) and prostaglandin E2 (PGE2) production in lipopolysaccharide (LPS)-activated RAW 264.7 macrophages.MEO was found to be significantly active against human lung carcinoma SPC-A1, human leukemia K562 and human gastric cancer SGC-7901 cells, with an IC50 value of 10.89, 16.16 and 38.76 µg/ml, respectively.The results of this study may provide an experimental basis for further systematic research, rational development and clinical utilization of peppermint resources.

View Article: PubMed Central - PubMed

Affiliation: State Key Laboratory of New Drug & Pharmaceutical Process, Shanghai Institute of Pharmaceutical Industry, China State Institute of Pharmaceutical Industry, Shanghai, 200040, China.

ABSTRACT
The chemical composition, anti-inflammatory, cytotoxic and antioxidant activities of essential oil from leaves of Mentha piperita (MEO) grown in China were investigated. Using GC-MS analysis, the chemical composition of MEO was characterized, showing that it was mainly composed of menthol, menthone and menthy acetate. MEO exhibited potent anti-inflammatory activities in a croton oil-induced mouse ear edema model. It could also effectively inhibit nitric oxide (NO) and prostaglandin E2 (PGE2) production in lipopolysaccharide (LPS)-activated RAW 264.7 macrophages. The cytotoxic effect was assessed against four human cancer cells. MEO was found to be significantly active against human lung carcinoma SPC-A1, human leukemia K562 and human gastric cancer SGC-7901 cells, with an IC50 value of 10.89, 16.16 and 38.76 µg/ml, respectively. In addition, MEO had moderate antioxidant activity. The results of this study may provide an experimental basis for further systematic research, rational development and clinical utilization of peppermint resources.

No MeSH data available.


Related in: MedlinePlus

Antioxidant activities of MEO, (A) scavenging of DPPH radical, (B) scavenging of hydroxyl radical, (C) reducing power and (D) total antioxidant activity.Values were representative of three separated experiments.
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pone-0114767-g004: Antioxidant activities of MEO, (A) scavenging of DPPH radical, (B) scavenging of hydroxyl radical, (C) reducing power and (D) total antioxidant activity.Values were representative of three separated experiments.

Mentions: DPPH is a stable free radical and has been widely accepted as a tool for estimating free radical scavenging activities of antioxidants. As shown in Fig. 4A, solutions with MEO concentrations of 200, 400, 600, 800 and 1000 µg/ml were prepared to evaluate the DPPH radical scavenging capacity, and BHT was used as positive control. The respective scavenging capacities ranged from 36.81% to 79.85% for MEO, vs. 82.36% to 93.85% for BHT. The scavenging ability of MEO was observed in a dose-dependent manner. Although the scavenging capacity of MEO was lower than that of BHT at all tested concentrations, the scavenging rate reached almost 80% at 1000 µg/ml, indicating that MEO had an appreciable scavenging ability.


Chemical Composition and Anti-Inflammatory, Cytotoxic and Antioxidant Activities of Essential Oil from Leaves of Mentha piperita Grown in China.

Sun Z, Wang H, Wang J, Zhou L, Yang P - PLoS ONE (2014)

Antioxidant activities of MEO, (A) scavenging of DPPH radical, (B) scavenging of hydroxyl radical, (C) reducing power and (D) total antioxidant activity.Values were representative of three separated experiments.
© Copyright Policy
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC4262447&req=5

pone-0114767-g004: Antioxidant activities of MEO, (A) scavenging of DPPH radical, (B) scavenging of hydroxyl radical, (C) reducing power and (D) total antioxidant activity.Values were representative of three separated experiments.
Mentions: DPPH is a stable free radical and has been widely accepted as a tool for estimating free radical scavenging activities of antioxidants. As shown in Fig. 4A, solutions with MEO concentrations of 200, 400, 600, 800 and 1000 µg/ml were prepared to evaluate the DPPH radical scavenging capacity, and BHT was used as positive control. The respective scavenging capacities ranged from 36.81% to 79.85% for MEO, vs. 82.36% to 93.85% for BHT. The scavenging ability of MEO was observed in a dose-dependent manner. Although the scavenging capacity of MEO was lower than that of BHT at all tested concentrations, the scavenging rate reached almost 80% at 1000 µg/ml, indicating that MEO had an appreciable scavenging ability.

Bottom Line: It could also effectively inhibit nitric oxide (NO) and prostaglandin E2 (PGE2) production in lipopolysaccharide (LPS)-activated RAW 264.7 macrophages.MEO was found to be significantly active against human lung carcinoma SPC-A1, human leukemia K562 and human gastric cancer SGC-7901 cells, with an IC50 value of 10.89, 16.16 and 38.76 µg/ml, respectively.The results of this study may provide an experimental basis for further systematic research, rational development and clinical utilization of peppermint resources.

View Article: PubMed Central - PubMed

Affiliation: State Key Laboratory of New Drug & Pharmaceutical Process, Shanghai Institute of Pharmaceutical Industry, China State Institute of Pharmaceutical Industry, Shanghai, 200040, China.

ABSTRACT
The chemical composition, anti-inflammatory, cytotoxic and antioxidant activities of essential oil from leaves of Mentha piperita (MEO) grown in China were investigated. Using GC-MS analysis, the chemical composition of MEO was characterized, showing that it was mainly composed of menthol, menthone and menthy acetate. MEO exhibited potent anti-inflammatory activities in a croton oil-induced mouse ear edema model. It could also effectively inhibit nitric oxide (NO) and prostaglandin E2 (PGE2) production in lipopolysaccharide (LPS)-activated RAW 264.7 macrophages. The cytotoxic effect was assessed against four human cancer cells. MEO was found to be significantly active against human lung carcinoma SPC-A1, human leukemia K562 and human gastric cancer SGC-7901 cells, with an IC50 value of 10.89, 16.16 and 38.76 µg/ml, respectively. In addition, MEO had moderate antioxidant activity. The results of this study may provide an experimental basis for further systematic research, rational development and clinical utilization of peppermint resources.

No MeSH data available.


Related in: MedlinePlus