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Chemical Composition and Anti-Inflammatory, Cytotoxic and Antioxidant Activities of Essential Oil from Leaves of Mentha piperita Grown in China.

Sun Z, Wang H, Wang J, Zhou L, Yang P - PLoS ONE (2014)

Bottom Line: It could also effectively inhibit nitric oxide (NO) and prostaglandin E2 (PGE2) production in lipopolysaccharide (LPS)-activated RAW 264.7 macrophages.MEO was found to be significantly active against human lung carcinoma SPC-A1, human leukemia K562 and human gastric cancer SGC-7901 cells, with an IC50 value of 10.89, 16.16 and 38.76 µg/ml, respectively.The results of this study may provide an experimental basis for further systematic research, rational development and clinical utilization of peppermint resources.

View Article: PubMed Central - PubMed

Affiliation: State Key Laboratory of New Drug & Pharmaceutical Process, Shanghai Institute of Pharmaceutical Industry, China State Institute of Pharmaceutical Industry, Shanghai, 200040, China.

ABSTRACT
The chemical composition, anti-inflammatory, cytotoxic and antioxidant activities of essential oil from leaves of Mentha piperita (MEO) grown in China were investigated. Using GC-MS analysis, the chemical composition of MEO was characterized, showing that it was mainly composed of menthol, menthone and menthy acetate. MEO exhibited potent anti-inflammatory activities in a croton oil-induced mouse ear edema model. It could also effectively inhibit nitric oxide (NO) and prostaglandin E2 (PGE2) production in lipopolysaccharide (LPS)-activated RAW 264.7 macrophages. The cytotoxic effect was assessed against four human cancer cells. MEO was found to be significantly active against human lung carcinoma SPC-A1, human leukemia K562 and human gastric cancer SGC-7901 cells, with an IC50 value of 10.89, 16.16 and 38.76 µg/ml, respectively. In addition, MEO had moderate antioxidant activity. The results of this study may provide an experimental basis for further systematic research, rational development and clinical utilization of peppermint resources.

No MeSH data available.


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GC/MS of MEO.
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pone-0114767-g001: GC/MS of MEO.

Mentions: In MEO chromatographic analysis (Fig. 1), 51 compounds were identified based on comparison of the mass spectrum with the NIST08 database and the related literature [15], [16], [17], representing 96.53% of the total oil (Table 1). The MEO was characterized by the dominant presence of menthol (30.69%), menthone (14.51%) and menthy acetate (12.86%), in agreement with other authors, such as Lu et al, who identified menthol, menthone and menthy acetate occurring at 32.53%, 26.50% and 10.37% in samples collected in Jiangsu province, China, Xu et al, who found them at 45.52%, 17.21% and 4.41% in samples collected in Xinjiang Province in China [15], [16]. Other significant constituents in MEO included neomenthol (9.26%), pulegone (4.36%), cineol (2.91%) and caryophyllene (2.52%). Although the contents of these main constituents in essential oil derived from M. Piperta grown in China were similar, there is a difference in chemical composition of MEO compared with those grown in other regions of the world. In essential oil from M. Piperta collected in Iran, researchers reported α-teripene as the major constituent (19.7%), followed by pipertitinone oxide (19.7%) and isomenthone (10.3%) [9]. The main constituents in MEO derived from India were reported to be limonene (18.4%), α-pinene (17.3%) and β-pinene (13.9%) [17]. However, we did not find menthofuran in MEO in the present study. This variation in chemical composition may be attributed to various factors in growing conditions, such as temperature, humidity, radiation, climate and harvest seasons.


Chemical Composition and Anti-Inflammatory, Cytotoxic and Antioxidant Activities of Essential Oil from Leaves of Mentha piperita Grown in China.

Sun Z, Wang H, Wang J, Zhou L, Yang P - PLoS ONE (2014)

GC/MS of MEO.
© Copyright Policy
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC4262447&req=5

pone-0114767-g001: GC/MS of MEO.
Mentions: In MEO chromatographic analysis (Fig. 1), 51 compounds were identified based on comparison of the mass spectrum with the NIST08 database and the related literature [15], [16], [17], representing 96.53% of the total oil (Table 1). The MEO was characterized by the dominant presence of menthol (30.69%), menthone (14.51%) and menthy acetate (12.86%), in agreement with other authors, such as Lu et al, who identified menthol, menthone and menthy acetate occurring at 32.53%, 26.50% and 10.37% in samples collected in Jiangsu province, China, Xu et al, who found them at 45.52%, 17.21% and 4.41% in samples collected in Xinjiang Province in China [15], [16]. Other significant constituents in MEO included neomenthol (9.26%), pulegone (4.36%), cineol (2.91%) and caryophyllene (2.52%). Although the contents of these main constituents in essential oil derived from M. Piperta grown in China were similar, there is a difference in chemical composition of MEO compared with those grown in other regions of the world. In essential oil from M. Piperta collected in Iran, researchers reported α-teripene as the major constituent (19.7%), followed by pipertitinone oxide (19.7%) and isomenthone (10.3%) [9]. The main constituents in MEO derived from India were reported to be limonene (18.4%), α-pinene (17.3%) and β-pinene (13.9%) [17]. However, we did not find menthofuran in MEO in the present study. This variation in chemical composition may be attributed to various factors in growing conditions, such as temperature, humidity, radiation, climate and harvest seasons.

Bottom Line: It could also effectively inhibit nitric oxide (NO) and prostaglandin E2 (PGE2) production in lipopolysaccharide (LPS)-activated RAW 264.7 macrophages.MEO was found to be significantly active against human lung carcinoma SPC-A1, human leukemia K562 and human gastric cancer SGC-7901 cells, with an IC50 value of 10.89, 16.16 and 38.76 µg/ml, respectively.The results of this study may provide an experimental basis for further systematic research, rational development and clinical utilization of peppermint resources.

View Article: PubMed Central - PubMed

Affiliation: State Key Laboratory of New Drug & Pharmaceutical Process, Shanghai Institute of Pharmaceutical Industry, China State Institute of Pharmaceutical Industry, Shanghai, 200040, China.

ABSTRACT
The chemical composition, anti-inflammatory, cytotoxic and antioxidant activities of essential oil from leaves of Mentha piperita (MEO) grown in China were investigated. Using GC-MS analysis, the chemical composition of MEO was characterized, showing that it was mainly composed of menthol, menthone and menthy acetate. MEO exhibited potent anti-inflammatory activities in a croton oil-induced mouse ear edema model. It could also effectively inhibit nitric oxide (NO) and prostaglandin E2 (PGE2) production in lipopolysaccharide (LPS)-activated RAW 264.7 macrophages. The cytotoxic effect was assessed against four human cancer cells. MEO was found to be significantly active against human lung carcinoma SPC-A1, human leukemia K562 and human gastric cancer SGC-7901 cells, with an IC50 value of 10.89, 16.16 and 38.76 µg/ml, respectively. In addition, MEO had moderate antioxidant activity. The results of this study may provide an experimental basis for further systematic research, rational development and clinical utilization of peppermint resources.

No MeSH data available.


Related in: MedlinePlus